Fluorescence Microscopy And Fluorescent Probes
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Fluorescence Microscopy And Fluorescent Probes
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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Fluorescence Microscopy And Fluorescent Probes
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Author : Jan Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-08-31
Fluorescence Microscopy And Fluorescent Probes written by Jan Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-08-31 with Science categories.
The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser confocal fluorescence microscopy. Six invited review lectures illuminate these recent developments and their role in cell biology, including computer simulations of molecular behavior. The 34 other papers focus on the major themes of: new developments in fluorescence instrumentation; fluorescent probes; nucleic acid labels; and other fluorescent labels, markers, and fluorogenic substrates. Annotation copyrighted by Book News, Inc., Portland, OR
Fluorescent And Luminescent Probes For Biological Activity
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Author : W. T. Mason
language : en
Publisher: Elsevier
Release Date : 1999-04-16
Fluorescent And Luminescent Probes For Biological Activity written by W. T. Mason and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-04-16 with Science categories.
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy
Fluorescence Microscopy And Fluorescent Probes
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Author : J. Slavik
language : en
Publisher:
Release Date : 2014-01-15
Fluorescence Microscopy And Fluorescent Probes written by J. Slavik and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
Fluorescence Microscopy
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Author : Ulrich Kubitscheck
language : en
Publisher: John Wiley & Sons
Release Date : 2017-06-19
Fluorescence Microscopy written by Ulrich Kubitscheck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-19 with Medical categories.
While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.
Recent Advances In Fluorescent Probes For Super Resolution Microscopy
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Author : Chayan Kanti Nandi
language : en
Publisher: Frontiers Media SA
Release Date : 2021-07-21
Recent Advances In Fluorescent Probes For Super Resolution Microscopy written by Chayan Kanti Nandi and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-21 with Science categories.
Fluorescent Probes
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Author :
language : en
Publisher: Academic Press
Release Date : 2021-05-13
Fluorescent Probes written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-05-13 with Science categories.
Fluorescent Probes, Volume 48 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on important topics, including Hydrogel microarray technology as a tool for clinical diagnostics, The use of probes and bacteriophages for bacteria detection, Probes used with point-of-care microfluidic devices for pathogen detection, Methods for combining FIB/SEM with three-dimensional fluorescence microscopy using CLEM approaches, Probes and Microbes, Microbial signatures associated with cancers, Fluorescent Aptamers for Detection and Treatment of Pathogenic Bacteria and Cancer, Labelled and Unlabeled Probes for Pathogen Detection with Molecular Biology Methods and Biosensors, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Microbiology series
Fluorescence Spectroscopy Imaging And Probes
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Author : Ruud Kraayenhof
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Fluorescence Spectroscopy Imaging And Probes written by Ruud Kraayenhof and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.
Fluorescence Microscopy
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Author : F. W. D. Rost
language : en
Publisher: Cambridge University Press
Release Date : 1992
Fluorescence Microscopy written by F. W. D. Rost and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Science categories.
Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.
Fluorescence Probes In Oncology
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Author : Elli Kohen
language : en
Publisher: World Scientific
Release Date : 2002-05-20
Fluorescence Probes In Oncology written by Elli Kohen and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-05-20 with Medical categories.
This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book. Contents:The Nature of LightIntroduction to PhotophysicsBasic Approaches to the Experimental Study of Chemical and Biological LuminescenceMethods and InstrumentationNew Methods. Theoretical Basis and Potential Applications to BiologyFluorescent ProbesApplications of Fluorescence Techniques to Study Biological Processes in Normal and Pathological CellsCell Physiopathology Readership: Students and researchers in oncology, the life sciences, biology and optics. Keywords:Chemiluminescence;Bioluminescence;Green Fluorescent Protein (GFP);Microspectrofluorimetry;Fluorescence Resonance Energy Transfer (FRET);Fluorescence Lifetime Imaging (FLIM);Fluorescence In Situ Hybridization (FISH);Multicolor Spectral Karyotyping (SKY);Fourier Interferometric Spectral Imaging;Fluorescence Imaging;Fluorescence Probe;Oncology;Cell Biology;Pathology;Diagnostics