Recent Advances In Fluorescent Probes For Super Resolution Microscopy
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Recent Advances In Fluorescent Probes For Super Resolution Microscopy
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Author : Chayan Kanti Nandi
language : en
Publisher: Frontiers Media SA
Release Date : 2021-07-21
Recent Advances In Fluorescent Probes For Super Resolution Microscopy written by Chayan Kanti Nandi and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-21 with Science categories.
Super Resolution Optical Imaging And Microscopy
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Author : Junle Qu
language : en
Publisher: John Wiley & Sons
Release Date : 2023-12-11
Super Resolution Optical Imaging And Microscopy written by Junle Qu and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-12-11 with Science categories.
Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.
Super Resolution Microscopy
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Author : Udo J. Birk
language : en
Publisher: John Wiley & Sons
Release Date : 2017-09-12
Super Resolution Microscopy written by Udo J. Birk and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-09-12 with Science categories.
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Far Field Optical Nanoscopy
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Author : Philip Tinnefeld
language : en
Publisher: Springer
Release Date : 2015-02-07
Far Field Optical Nanoscopy written by Philip Tinnefeld and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-02-07 with Science categories.
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Advances In Molecular Biophotonics
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Author : Yong Deng
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2017-04-24
Advances In Molecular Biophotonics written by Yong Deng and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-24 with Science categories.
Being the most active field in modern physics, Optical Physics has developed many new branches and interdisciplinary fields overlapping with various classical disciplines. This series summarizes the advancements of optical physics in the past twenty years in the following fields: High Field Laser Physics, Precision Laser Spectroscopy, Nonlinear Optics, Nanophotonics, Quantum Optics, Ultrafast Optics, Condensed Matter Optics, and Molecular Biophotonics.
Fluorescence Microscopy And Fluorescent Probes
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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Fluorescence Microscopy
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Author : Ulrich Kubitscheck
language : en
Publisher: John Wiley & Sons
Release Date : 2017-06-19
Fluorescence Microscopy written by Ulrich Kubitscheck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-19 with Medical categories.
While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.
Fluorescence Microscopy And Fluorescent Probes
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Author : Jan Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-08-31
Fluorescence Microscopy And Fluorescent Probes written by Jan Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-08-31 with Science categories.
The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser confocal fluorescence microscopy. Six invited review lectures illuminate these recent developments and their role in cell biology, including computer simulations of molecular behavior. The 34 other papers focus on the major themes of: new developments in fluorescence instrumentation; fluorescent probes; nucleic acid labels; and other fluorescent labels, markers, and fluorogenic substrates. Annotation copyrighted by Book News, Inc., Portland, OR
Recent Advances In Super Resolution Microscopy Imaging
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Author : Aleksei Anatoliyovych Stepanenko
language : en
Publisher: Delve Publishing
Release Date : 2017-11
Recent Advances In Super Resolution Microscopy Imaging written by Aleksei Anatoliyovych Stepanenko and has been published by Delve Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11 with categories.
The fluorescence microscope is the principal tool for investigating the spatial organization of bio-molecular processes. Instrumentation and techniques have evolved considerably since the first realizations of fluorescence microscopy. The technology is constantly being advanced to image faster, deeper, less invasively, and more quantitatively, driving discovery of both biological and clinical mechanisms. In this book, we discuss the various approaches, which can be successfully used in live cells, the tradeoffs in resolution, speed, and ease of implementation, which one must make for each approach, and the quality of results that one might expect from each technique. We introduce the recent development of optical microscopy that has achieved spatial resolution beyond the diffraction limit. Super-resolution imaging techniques outperforming the classical diffraction limits are photoactivated localization microscopy (PALM), stochastic optical resolution microscopy (STORM), stimulated-emission depletion microscopy (STED), three-dimensional structured illumation microscopy (3D-SIM) and total internal reflection fluorescence microscopy (TIRF). These super-resolution microscopy techniques have permitted detailed studies of organelles, vesicles, microtubules, DNAs, proteins, and other biochemical particles on the nanometer scale. Super-resolution microscopy has opened the door to true nanoscale mapping of biological components.
Superresolution Optical Microscopy
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Author : Barry R. Masters
language : en
Publisher: Springer Nature
Release Date : 2020-03-21
Superresolution Optical Microscopy written by Barry R. Masters and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-21 with Science categories.
This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.