Single Molecule Techniques
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Single Molecule Techniques
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Author : Paul R. Selvin
language : en
Publisher: CSHL Press
Release Date : 2008
Single Molecule Techniques written by Paul R. Selvin and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.
Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.
Handbook Of Single Molecule Biophysics
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Author : Peter Hinterdorfer
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-12-24
Handbook Of Single Molecule Biophysics written by Peter Hinterdorfer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-12-24 with Science categories.
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Single Molecule Optical Detection Imaging And Spectroscopy
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Author : T. Basché
language : en
Publisher: Wiley-VCH
Release Date : 1997
Single Molecule Optical Detection Imaging And Spectroscopy written by T. Basché and has been published by Wiley-VCH this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Science categories.
Single Molecule Spectroscopy is one of the hottest topics in today's chemistry. It brings us close to the the most exciting vision generations of chemists have been dreaming of: To observe and examine single molecules! While most of chemistry deals with myriads of molecules, this books presents the latest developments for the detection and investigation of single entities. Written by internationally renowned authors, it is a thorough and comprehensive survey of current methods and their applications.
Single Molecule Spectroscopy
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Author : R. Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Single Molecule Spectroscopy written by R. Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
One often hears that nanoscience or, in other words, the knowledge and control of matter at length scales of a few nanometers, will be the scientific frontier of the 21st century. Although it has become almost commonplace, this prediction deserves some justification. The technological and scientific stakes of nanoscience indeed encompass many fields of science: they include the ultimate miniaturization of electronic devices to acquire, store, and process information, and also such basic endeavors as understanding the microscopic processes and patterns responsible for the physical properties of materials, or the many unsolved questions raised by the astoundingly intricate workings of living matter. Although the dream of observing and controlling matter at molecular scales is nearly as old as the very concept of molecules, earlier attempts at practical realizations were hampered by a scarcity of suitable access to the nanoworld. During the last two decades of the 20th century, owing to the several new tools which have been developed to address objects at nanometer scales, the nanoworld appears closer than ever, within our reach! A major class of methods in nanoscience are local probe microscopies such as scanning tunnelling or atomic force microscopies. They require scanning a sharp tip with molecular dimensions across the surface of the sample under study and, by direct action of the tip on the sample, they make nano-manipulations possible. The present book is devoted to another class of methods, the selection and study of single, optically active nano-objects by purely optical means.
Single Molecule Dynamics In Life Science
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Author : Toshio Yanagida
language : en
Publisher: John Wiley & Sons
Release Date : 2008-11-24
Single Molecule Dynamics In Life Science written by Toshio Yanagida and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-24 with Science categories.
In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.
Handbook Of Fluorescence Spectroscopy And Imaging
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Author : Markus Sauer
language : en
Publisher: John Wiley & Sons
Release Date : 2010-12-23
Handbook Of Fluorescence Spectroscopy And Imaging written by Markus Sauer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-23 with Science categories.
Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Methods In Molecular Biophysics
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Author : Igor N. Serdyuk
language : en
Publisher: Cambridge University Press
Release Date : 2017-05-18
Methods In Molecular Biophysics written by Igor N. Serdyuk and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-18 with Medical categories.
A comprehensive graduate textbook explaining key physical methods in biology, reflecting the very latest research in this fast-moving field.
Single Molecule Analysis
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Author : Erwin J. G. Peterman
language : en
Publisher: Humana Press
Release Date : 2011-09-15
Single Molecule Analysis written by Erwin J. G. Peterman and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-15 with Science categories.
Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.
Single Molecule Enzymology Nanomechanical Manipulation And Hybrid Methods
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Author :
language : en
Publisher: Academic Press
Release Date : 2017-01-03
Single Molecule Enzymology Nanomechanical Manipulation And Hybrid Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-03 with Science categories.
Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods in single-molecule enzymology - Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion
Single Molecule Biology
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Author : Alexander E. Knight
language : en
Publisher: Academic Press
Release Date : 2009-02-26
Single Molecule Biology written by Alexander E. Knight and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-02-26 with Science categories.
Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. - Organized by biological system or molecule - Each chapter discusses insights into mechanism of action, structure, and function - Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc - Includes an introduction to key principles and an historical overview - Discusses applications in molecular diagnostics and drug discovery - Provides an expert's perspective on future development