Single Molecule Analysis
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Single Molecule Analysis
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Author : Iddo Heller
language : en
Publisher: Humana
Release Date : 2023-11-09
Single Molecule Analysis written by Iddo Heller and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-11-09 with Science categories.
This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Single Molecule Image Analysis
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Author : Christian Franke
language : en
Publisher: Frontiers Media SA
Release Date : 2022-12-15
Single Molecule Image Analysis written by Christian Franke and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-15 with Science categories.
Single Molecule Studies Of Proteins
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Author : Andres F. Oberhauser
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-11-09
Single Molecule Studies Of Proteins written by Andres F. Oberhauser and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-09 with Science categories.
In Single Molecule Studies of Proteins, expert researchers discuss the successful application of single-molecule techniques to a wide range of biological events, such as the imaging and mapping of cell surface receptors, the analysis of the unfolding and folding pathways of single proteins, the analysis interaction forces between biomolecules, the study of enzyme catalysis or the visualization of molecular motors in action. The chapters are aimed at established investigators and post-doctoral researchers in the life sciences wanting to pursue research in the various areas in which single-molecule approaches are important; this volume also remains accessible to advanced graduate students seeking similar research goals.
Cell Signaling Reactions
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Author : Yasushi Sako
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-11-04
Cell Signaling Reactions written by Yasushi Sako and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-04 with Medical categories.
This book encompasses the exciting developments and challenges in the fast-moving and rapidly expanding research field of single-molecule kinetic analysis of cell signaling that promises to be one of the most significant and exciting areas of biological research for the foreseeable future. Cell signaling is carried out by complicated reaction networks of macromolecules, and single-molecule analyses has already demonstrated its power to unravel complex reaction dynamics in purified systems. To date, most of the published research in the field of single-molecule processes in cells, focus on the dynamic properties (translational movements of the centre of mass) of biological molecules. However, we hope that this book presents as many kinetic analyses of cell signaling as possible. Although single-molecule kinetic analysis of cellular systems is a relatively young field when compared with the analysis of single-molecule movements in cells, this type of analysis is highly important because it directly relates to the molecular functions that control cellular behavior and in the future, single-molecule kinetic analysis will be largely directed towards cellular systems. Thus, we hope that this book will be of interest to all those working in the fields of molecular and cell biology, as well as biophysics and biochemistry.
Single Molecule Enzymology
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Author : Gregory I. Mashanov
language : en
Publisher: Humana Press
Release Date : 2011-08-04
Single Molecule Enzymology written by Gregory I. Mashanov and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-04 with Science categories.
The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.
Handbook Of Single Molecule Biophysics
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Author : Peter Hinterdorfer
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-12-24
Handbook Of Single Molecule Biophysics written by Peter Hinterdorfer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-12-24 with Science categories.
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Theory And Evaluation Of Single Molecule Signals
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Author : Eli Barkai
language : en
Publisher: World Scientific
Release Date : 2008
Theory And Evaluation Of Single Molecule Signals written by Eli Barkai and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.
This book reviews recently developed theoretical and numerical approaches to deal with optical and mechanical signals from individual molecules. The character of data generated by single molecules, and more generally by single nano-objects, qualitatively differs from those obtained in conventional experiments on large ensembles of molecules. Fluctuations, randomness and irreproducibility are central to single-molecule measurements, and the specific methods required to extract reliable and statistically relevant information from them are presented here. With contributions mainly from participants of the ?Theory, Modeling and Evaluation of Single-Molecule Measurements? workshop held in Leiden, the Netherlands, on April 16-20, 2007, this book is an authoritative compendium on the subject.
Single Molecule Dynamics In Life Science
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Author : Toshio Yanagida
language : en
Publisher: John Wiley & Sons
Release Date : 2008-12-04
Single Molecule Dynamics In Life Science written by Toshio Yanagida and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-04 with Science categories.
In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.
Single Molecule Analysis Of Cancer Genomes
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Author :
language : en
Publisher:
Release Date : 2012
Single Molecule Analysis Of Cancer Genomes written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Single Molecule Techniques
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Author : Paul R. Selvin
language : en
Publisher: CSHL Press
Release Date : 2008
Single Molecule Techniques written by Paul R. Selvin and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Atomic force microscopy categories.
Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.