Single Molecule Optical Detection Imaging And Spectroscopy
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Single Molecule Optical Detection Imaging And Spectroscopy
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Author : W. E. Moerner
language : en
Publisher: John Wiley & Sons
Release Date : 2008-09-26
Single Molecule Optical Detection Imaging And Spectroscopy written by W. E. Moerner and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-26 with Science categories.
Single Molecule Spectroscopy is one of the hottest topics in today's chemistry. It brings us close to the the most exciting vision generations of chemists have been dreaming of: To observe and examine single molecules! While most of chemistry deals with myriads of molecules, this books presents the latest developments for the detection and investigation of single entities. Written by internationally renowned authors, it is a thorough and comprehensive survey of current methods and their applications.
Single Molecule Spectroscopy
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Author : R. Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Single Molecule Spectroscopy written by R. Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
One often hears that nanoscience or, in other words, the knowledge and control of matter at length scales of a few nanometers, will be the scientific frontier of the 21st century. Although it has become almost commonplace, this prediction deserves some justification. The technological and scientific stakes of nanoscience indeed encompass many fields of science: they include the ultimate miniaturization of electronic devices to acquire, store, and process information, and also such basic endeavors as understanding the microscopic processes and patterns responsible for the physical properties of materials, or the many unsolved questions raised by the astoundingly intricate workings of living matter. Although the dream of observing and controlling matter at molecular scales is nearly as old as the very concept of molecules, earlier attempts at practical realizations were hampered by a scarcity of suitable access to the nanoworld. During the last two decades of the 20th century, owing to the several new tools which have been developed to address objects at nanometer scales, the nanoworld appears closer than ever, within our reach! A major class of methods in nanoscience are local probe microscopies such as scanning tunnelling or atomic force microscopies. They require scanning a sharp tip with molecular dimensions across the surface of the sample under study and, by direct action of the tip on the sample, they make nano-manipulations possible. The present book is devoted to another class of methods, the selection and study of single, optically active nano-objects by purely optical means.
Single Molecule Optical Detection Imaging And Spectroscopy
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Author : T. Basché
language : en
Publisher: Wiley-VCH
Release Date : 1997
Single Molecule Optical Detection Imaging And Spectroscopy written by T. Basché and has been published by Wiley-VCH this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Science categories.
Single Molecule Spectroscopy is one of the hottest topics in today's chemistry. It brings us close to the the most exciting vision generations of chemists have been dreaming of: To observe and examine single molecules! While most of chemistry deals with myriads of molecules, this books presents the latest developments for the detection and investigation of single entities. Written by internationally renowned authors, it is a thorough and comprehensive survey of current methods and their applications.
Handbook Of Fluorescence Spectroscopy And Imaging
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Author : Markus Sauer
language : en
Publisher: John Wiley & Sons
Release Date : 2010-12-23
Handbook Of Fluorescence Spectroscopy And Imaging written by Markus Sauer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-23 with Science categories.
Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Fluorescence Correlation Spectroscopy
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Author : R. Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Fluorescence Correlation Spectroscopy written by R. Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Fluorescence correlation spectroscopy (FCS) was developed in order to char acterize the dynamics of molecular processes in systems in thermodynamic equilibrium. FCS determines transport and chemical reaction rates from mea surements of spontaneous microscopic thermally driven molecular concentra tion fluctuations. Since its inception, and particularly in recent years, techni cal and conceptual advances have extended the range of practical applicability and the information obtainable from FCS measurements. Improvements in microscopy, data acquisition, and data processing have greatly shortened the time required for FCS measurements. FCS can now be routinely applied to labile systems such as cells, and for the acquisition of large volumes of data as required for high-throughput screening. Cross correlation methods pro vide a powerful tool for characterizing interactions among different molecular species. Analysis of the amplitude of concentration fluctuations can provide a wealth of information about aggregation/polymerization process and the compositions of mixtures. Furthermore, FCS provides a bridge between conventional measurements of dynamic processes on a macroscopic concentration scale and the currently developing field of single molecule measurements. Both FCS and single mole cule approaches measure directly stochastic fluctuations in molecular pro perties, and so must be analyzed by statistical methods to yield conventional phenomenological parameters. As commonly practiced, FCS yields these phe nomenological parameters, e. g. , diffusion coefficients and chemical rate con stants, directly in terms of a fluorescence fluctuation autocorrelation func tion.
Handbook Of Single Molecule Biophysics
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Author : Peter Hinterdorfer
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-12-24
Handbook Of Single Molecule Biophysics written by Peter Hinterdorfer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-12-24 with Science categories.
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Handbook Of Spectroscopy
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Author : G¿nter Gauglitz
language : de
Publisher: John Wiley & Sons
Release Date : 2006-03-06
Handbook Of Spectroscopy written by G¿nter Gauglitz and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.
This handbook provides a straightforward introduction to spectroscopy, showing what it can do and how it does it, together with a clear, integrated and objective account of the wealth of information that can be derived from spectra. The sequence of chapters covers a wide range of the electromagnetic spectrum, and the physical processes involved, from nuclear phenomena to molecular rotation processes. - A day-by-day laboratory guide: its design based on practical knowledge of spectroscopists at universities, industries and research institutes - A well-structured information source containing methods and applications sections framed by sections on general topics - Guides users to a decision about which spectroscopic method and which instrumentation will be the most appropriate to solve their own practical problem - Rapid access to essential information - Correct analysis of a huge number of measured spectra data and smart use of such information sources as databases and spectra libraries
Advances In Biophotonics
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Author : Brian Wilson
language : en
Publisher: IOS Press
Release Date : 2005
Advances In Biophotonics written by Brian Wilson and has been published by IOS Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Electronic books categories.
Microsystem Technology
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Author : Hans-Peter Saluz
language : en
Publisher: Birkhäuser
Release Date : 2012-12-06
Microsystem Technology written by Hans-Peter Saluz and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Biomolecular studies are the trial of Man to understand how Nature manages information at the molecular level. The understanding of molecular informa tion handling in nature is essential for the molecular optimization in chem istry, molecular biology, molecular pharmacology and therefore - as an ex ample - for the development of specifically acting drugs. The famous recent method of technical information management is digital electronics. Over the past few years, evidence has arisen that computerized and molecular information managements have many similar and overlapping aspects. For example, both technology and nature use digitized information and both use small structures for the efficient handling of information. Furthermore, they optimize their processes in order to gain a maximum of information with a minimum of invested energy. During the last two decades, novel experimental techniques in biomolec ular sciences have paved the way for artificial biomolecular optimization. In the same time interval, the progress of micro system technology has been extended from the field of digital electronics and sensing to micro liquid hand ling, and the field of chip-supported substance handling began. It appears that the "marriage" of physical micro technology and molecular processing will be consummated soon. The contact of both fields has been realized in for ex ample DNA chips. Such connections will also become relevant in additional fields in the near future. Biomolecular investigations are the first to profit from these fast growing scientific and technical connections between micro systems and molecular sciences.
Theory And Evaluation Of Single Molecule Signals
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Author : Eli Barkai
language : en
Publisher: World Scientific
Release Date : 2008
Theory And Evaluation Of Single Molecule Signals written by Eli Barkai and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.
This book reviews recently developed theoretical and numerical approaches to deal with optical and mechanical signals from individual molecules. The character of data generated by single molecules, and more generally by single nano-objects, qualitatively differs from those obtained in conventional experiments on large ensembles of molecules. Fluctuations, randomness and irreproducibility are central to single-molecule measurements, and the specific methods required to extract reliable and statistically relevant information from them are presented here. With contributions mainly from participants of the OC Theory, Modeling and Evaluation of Single-Molecule MeasurementsOCO workshop held in Leiden, the Netherlands, on April 16-20, 2007, this book is an authoritative compendium on the subject.