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Patch Clamp Analysis


Patch Clamp Analysis
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Patch Clamp Analysis


Patch Clamp Analysis
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Author : Wolfgang Walz
language : en
Publisher: Humana
Release Date : 2007-08-02

Patch Clamp Analysis written by Wolfgang Walz and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-02 with Medical categories.


Continuing the research of the best-selling first edition, this second edition collects three more years of research in the ever-expanding study of the cell membrane. It covers the latest developments in the "traditional" patch techniques. This authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field. Thorough and timely, this edition is an invaluable resource.



Patch Clamp Analysis


Patch Clamp Analysis
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Author : Wolfgang Walz
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-06

Patch Clamp Analysis written by Wolfgang Walz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-06 with Medical categories.


Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.



Patch Clamp Techniques


Patch Clamp Techniques
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Author : Yasunobu Okada
language : en
Publisher: Springer
Release Date : 2012-04-27

Patch Clamp Techniques written by Yasunobu Okada and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-27 with Medical categories.


Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.



Patch Clamp Electrophysiology


Patch Clamp Electrophysiology
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Author : Mark Dallas
language : en
Publisher: Humana
Release Date : 2020-10-30

Patch Clamp Electrophysiology written by Mark Dallas and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-30 with Medical categories.


This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.



Dynamic Clamp


Dynamic Clamp
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Author : Alain Destexhe
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-03-11

Dynamic Clamp written by Alain Destexhe and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-11 with Medical categories.


Dynamic-clamp is a fascinating electrophysiology technique that consists of merging living neurons with computational models. The dynamic-clamp (also called “conductance injection”) allows experimentalists and theoreticians to challenge neurons (or any other type of cell) with complex conductance stimuli generated by a computer. The technique can be implemented from neural simulation environments and a variety of custom-made or commercial systems. The real-time interaction between the computer and cell also enables the design of recording paradigms with unprecedented accuracy via a computational model of the electrode. Dynamic-Clamp: From Principles to Applications contains contributions from leading researchers in the field, who investigate these paradigms at the cellular or network level, in vivo and in vitro, and in different brain regions and cardiac cells. Topics discussed include the addition of artificially-generated synaptic activity to neurons; adding, amplifying or neutralizing voltage-dependent conductances; creating hybrid networks with real and artificial cells; attaching simulated dendritic tree structures to the living cell; and connecting different neurons. This book will be of interest to experimental biophysicists, neurophysiologists, and cardiac physiologists, as well as theoreticians, engineers, and computational neuroscientists. Graduate and undergraduate students will also find up-to-date coverage of physiological problems and how they are investigated.



Practical Methods In Cardiovascular Research


Practical Methods In Cardiovascular Research
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Author : Stefan Dhein
language : en
Publisher: Springer
Release Date : 2006-02-28

Practical Methods In Cardiovascular Research written by Stefan Dhein and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-02-28 with Medical categories.


Scientists working or planning to work in the field of cardiovascular research will welcome Methods in Cardiovascular Research as the reference book they have been waiting for. Not only general aspects of cardiovascular research are well presented but also detailed descriptions of methods, protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Methods in Cardiovascular Research is a "must have" for anybody with an interest in cardiovascular research.



Patch Clamp Technique


Patch Clamp Technique
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Author : Fatima Shad Kaneez
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-03-23

Patch Clamp Technique written by Fatima Shad Kaneez and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-23 with Science categories.


This book is a stimulating and interesting addition to the collected works on Patch clamp technique. Patch Clamping is an electrophysiological technique, which measures the electric current generated by a living cell, due to the movement of ions through the protein channels present in the cell membrane. The technique was developed by two German scientists, Erwin Neher and Bert Sakmann, who received the Nobel Prize in 1991 in Physiology for this innovative work. Patch clamp technique is used for measuring drug effect against a series of diseases and to find out the mechanism of diseases in animals and plants. It is also most useful in finding out the structure function activities of compounds and drugs, and most leading pharmaceutical companies used this technique for their drugs before bringing them for clinical trial. This book deals with the understanding of endogenous mechanisms of cells and their receptors as well as advantages of using this technique. It covers the basic principles and preparation types and also deals with the latest developments in the traditional patch clamp technique. Some chapters in this book take the technique to a next level of modulation and novel approach. This book will be of good value for students of physiology, neuroscience, cell biology and biophysics.



Electrophysiological Analysis Of Synaptic Transmission


Electrophysiological Analysis Of Synaptic Transmission
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Author : Nicholas Graziane
language : en
Publisher: Springer Nature
Release Date : 2022-08-03

Electrophysiological Analysis Of Synaptic Transmission written by Nicholas Graziane and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-08-03 with Medical categories.


This second edition volume expands on the previous edition with discussions on the latest techniques used to study synaptic transmissions. The chapters in this book are organized into six parts. Part One looks at the basic concepts, such as extracellular and intracellular recordings, and spatiotemporal effects of synaptic currents. Part Two describes the recording of synaptic currents, such as measuring kinetics of synaptic current and measuring reversal potentials. Part Three discusses basic experimentations of synaptic transmission and covers run-up and run-down, and amplitude. Parts Four and Five cover experimentations with computational components and molecular and visual components, such as measurement of a single synapse and electrophysiological and visual tags. Part Six explores in vivo recordings and talks about general considerations for in vivo exploration of synaptic plasticity. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and thorough, Electrophysiological Analysis of Synaptic Transmission, Second Edition is a valuable resource that introduces graduate students and postdoctoral fellows to important topics in this field and also expands these topics to practical electrophysiological approaches.



Encyclopedia Of Computational Neuroscience


Encyclopedia Of Computational Neuroscience
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Author : Dieter Jaeger
language : en
Publisher:
Release Date :

Encyclopedia Of Computational Neuroscience written by Dieter Jaeger and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with Computational neuroscience categories.




Patch Clamping


Patch Clamping
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Author : Areles Molleman
language : en
Publisher: John Wiley & Sons
Release Date : 2003-06-13

Patch Clamping written by Areles Molleman and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-06-13 with Science categories.


Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns