Coronavirus Replication And Reverse Genetics
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Coronavirus Replication And Reverse Genetics
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Author : Luis Enjuanes
language : en
Publisher: Springer Science & Business Media
Release Date : 2005-10-25
Coronavirus Replication And Reverse Genetics written by Luis Enjuanes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-10-25 with Medical categories.
Human coronaviruses caused the SARS epidemic that infected more than 8000 people, killing about ten percent of them in 32 countries. This book provides essential information on these viruses and the development of vaccines to control coronavirus infections.
Coronavirus Replication And Reverse Genetics
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Author : Luis Enjuanes
language : en
Publisher:
Release Date : 2005
Coronavirus Replication And Reverse Genetics written by Luis Enjuanes and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Coronaviruses categories.
Coronaviruses are the RNA viruses with the largest genome known to date (27 to 32 kb). Members of this virus family affect most domestic animal species, causing important socio-economical losses, and also infect humans. Human coronaviruses were known to cause the winter common cold, a mild infection without important pathological consequences except in immuno-compromised patients. Recently, two new human coronaviruses have emerged, one causing the Severe and Acute Respiratory Syndrome (SARS) that infected more than 8000 individuals, leading to more than 800 deaths in 32 countries. This epidemic mobilized the World Health Organization, which launched travel restrictions to certain parts of the world for the first time in the last 50 years. The fact that coronaviruses, as many other viruses, crossed the species barrier to infect humans has posed a serious challenge to scientists involved in animal and human health. Control of coronavirus-induced diseases can only be the consequence of research on virus molecular biology and pathogenesis. This book contains information on virus genome structure, mechanism of replication and transcription, and the development of tools that make possible reverse genetic studies to understand virus-host interactions and the molecular basis of virus pathogenesis. The book also provides essential information for the development of classical and recombinant vaccines to control coronavirus infections.
Reverse Genetics Of Rna Viruses
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Author : Daniel R. Pérez
language : en
Publisher: Springer Nature
Release Date : 2023-12-08
Reverse Genetics Of Rna Viruses written by Daniel R. Pérez and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-12-08 with Medical categories.
This second edition volume expands on the previous edition with new and updated chapters that highlight the latest methods and approaches for the manipulation of RNA viruses. The chapters in this book explore the fundamental role in studying RNA viruses; identifying markers of host range, disease, and transmission; and aid readers with the further development of in silico computational biology tools and artificial intelligence algorithms that can help predict the emergence of certain pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Reverse Genetics of RNA Viruses: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this developing field.
Coronaviruses
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Author : Helena Jane Maier
language : en
Publisher: Humana
Release Date : 2016-10-05
Coronaviruses written by Helena Jane Maier and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-05 with Science categories.
This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.
Reverse Genetics Of Rna Viruses
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Author : Anne Bridgen
language : en
Publisher: John Wiley & Sons
Release Date : 2012-10-07
Reverse Genetics Of Rna Viruses written by Anne Bridgen and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-10-07 with Science categories.
Reverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. Since many severe viral human and animal pathogens are RNA viruses, including those responsible for polio, measles, rotaviral diarrhoea and influenza infections, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases. Reverse Genetics of RNA Viruses provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. Written by a team of international experts in the field, it provides a unique insight into how the field has developed, what problems are being addressed now and where applications may lead in the future. It will prove invaluable to bioscience, medical and veterinary students, those starting research in this area as well as other researchers and teachers needing to update their knowledge of this fast-moving field. An authoritative, comprehensive overview of reverse genetics in RNA Viruses. Includes numerous examples of cutting- edge applications of reverse genetics within each of the RNA viral groups. Written by a team of international experts, including some of the leading researchers in the field.
Biology Of Negative Strand Rna Viruses The Power Of Reverse Genetics
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Author : Yoshihiro Kawaoka
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Biology Of Negative Strand Rna Viruses The Power Of Reverse Genetics written by Yoshihiro Kawaoka and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Medical categories.
Negative-strand RNA viruses, so named because of the polarity of their genomic RNA to mRNA, include important human and non-human pathogens. This volume covers major advances in reverse genetics techniques over the past decade, state-of-the-art basic science and the clinical implications of experimental findings. This should rekindle interest in negative-strand RNA viruses among readers, including those in other disciplines, leading to further progress in understanding these important viruses and in developing effective measures of control.
Coronaviruses
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Author : Volker Thiel
language : en
Publisher:
Release Date : 2020-01-28
Coronaviruses written by Volker Thiel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-28 with Science categories.
Paperback. ISBN 978-1-912530-35-9. In this timely book, internationally renowned experts review literally every aspect of cutting edge coronavirus research providing the first coherent picture of the molecular and cellular biology since the outbreak of SARS in 2003. Essential reading for all coronavirologists as well as scientists working on other viruses of the respiratory and/or gastrointestinal tract.
Learning From Sars
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Author : Institute of Medicine
language : en
Publisher: National Academies Press
Release Date : 2004-04-26
Learning From Sars written by Institute of Medicine and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-04-26 with Medical categories.
The emergence of severe acute respiratory syndrome (SARS) in late 2002 and 2003 challenged the global public health community to confront a novel epidemic that spread rapidly from its origins in southern China until it had reached more than 25 other countries within a matter of months. In addition to the number of patients infected with the SARS virus, the disease had profound economic and political repercussions in many of the affected regions. Recent reports of isolated new SARS cases and a fear that the disease could reemerge and spread have put public health officials on high alert for any indications of possible new outbreaks. This report examines the response to SARS by public health systems in individual countries, the biology of the SARS coronavirus and related coronaviruses in animals, the economic and political fallout of the SARS epidemic, quarantine law and other public health measures that apply to combating infectious diseases, and the role of international organizations and scientific cooperation in halting the spread of SARS. The report provides an illuminating survey of findings from the epidemic, along with an assessment of what might be needed in order to contain any future outbreaks of SARS or other emerging infections.
Coronaviruses
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Author : Volker Thiel
language : en
Publisher:
Release Date : 2007
Coronaviruses written by Volker Thiel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Coronavirus infections categories.
In this timely book, internationally renowned experts review literally every aspect of cutting edge coronavirus research providing the first coherent picture of the molecular and cellular biology since the outbreak of SARS in 2003. The book is divided into two sections: Part I focuses on the molecular biology of the virus itself and includes topics such as coronavirus binding and entry, replicase gene function, cis-acting RNA elements, coronavirus discontinuous transcription, reverse genetics, genome packaging and molecular evolution. In Part II of the book, the focus is on molecular and cellu.
Molecular Biology Of The Sars Coronavirus
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Author : Sunil K. Lal
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-03-10
Molecular Biology Of The Sars Coronavirus written by Sunil K. Lal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-10 with Science categories.
SARS was the ?rst new plague of the twenty-?rst century. Within months, it spread worldwide from its “birthplace” in Guangdong Province, China, affecting over 8,000 people in 25 countries and territories across ?ve continents. SARS exposed the vulnerability of our modern globalised world to the spread of a new emerging infection. SARS (or a similar new emerging disease) could neither have spread so rapidly nor had such a great global impact even 50 years ago, and arguably, it was itself a product of our global inter-connectedness. Increasing af?uence and a demand for wild-game as exotic food led to the development of large trade of live animal and game animal markets where many species of wild and domestic animals were co-housed, providing the ideal opportunities for inter-species tra- mission of viruses and other microbes. Once such a virus jumped species and attacked humans, the increased human mobility allowed the virus the opportunity for rapid spread. An infected patient from Guangdong who stayed for one day at a hotel in Hong Kong led to the transmission of the disease to 16 other guests who travelled on to seed outbreaks of the disease in Toronto, Singapore, and Vietnam, as well as within Hong Kong itself. The virus exploited the practices used in modern intensive care of patients with severe respiratory disease and the weakness in infection control practices within our health care systems to cause outbreaks within hospitals, further amplifying the spread of the disease. Health-care itself has become a two-edged sword.