Counting Molecules Within Cells
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Counting Molecules Within Cells
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Author : Valerie Coffman
language : en
Publisher: Biota Publishing
Release Date : 2014-10-01
Counting Molecules Within Cells written by Valerie Coffman and has been published by Biota Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-01 with Science categories.
A fundamental component of quantitative cell biology is the ability to count molecules within cells. The numbers of molecules and stoichiometries are the basis for structural models of protein complexes and simulations of biological processes. A variety of methods exist for in vivo quantifications, but the focus of this volume is mainly on fluorescence methods. The two most popular methods are stepwise photobleaching and ratio comparison using a standard curve. With recent advances in genome editing techniques, most model organisms are amenable to inserting coding sequences for fluorescent proteins into native genetic loci, making quantification of proteins by fluorescence microscopy one of the most ubiquitous tools available to cell biologists. The acquisition and analysis methods range from simple to complex, and most have been validated by counting with multiple methods and other types of data. Researchers should be aware of sources of error in the acquisition and analysis, but the accuracy of these methods is high. Quantification by fluorescence microscopy has yielded valuable new insights into many aspects of cell biology, highlighting its place among the standard tools for molecular and cell biologists.
Minorities And Small Numbers From Molecules To Organisms In Biology
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Author : Takeharu Nagai
language : en
Publisher: Springer
Release Date : 2018-11-03
Minorities And Small Numbers From Molecules To Organisms In Biology written by Takeharu Nagai and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-03 with Science categories.
This book provides an accessible introduction to an exciting new field of life science in which the focus is on small numbers of molecules and minorities within cell populations and their significance for the understanding of biological phenomena. Numbers, or quantitative data, are attracting more attention in cell biology following, for example, determination of the absolute copy number of each protein species in each bacterial cell and the recognition of leader cells that drive collective cell migration. Within this context, the authors present recent advances in experimental techniques, biological findings, and theories. A variety of cutting-edge topics and issues are addressed, with explanation of the ways in which recent developments in the field cast light on seemingly straightforward but difficult-to-answer questions. Readers will learn that we are on the verge of a paradigm shift as the importance of cooperation among groups of molecules in live cells is acknowledged. The book is designed to be enjoyable to read and easy to understand. It will be of interest for a wide range of readers, including young researchers and undergraduate/high school students.
Cell Biology By The Numbers
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Author : Ron Milo
language : en
Publisher: Garland Science
Release Date : 2015-12-07
Cell Biology By The Numbers written by Ron Milo and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-07 with Science categories.
A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Introduction To Quantitative Cell Biology
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Author : Wallace F. Marshall
language : en
Publisher: Biota Publishing
Release Date : 2017-07-20
Introduction To Quantitative Cell Biology written by Wallace F. Marshall and has been published by Biota Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-20 with Science categories.
For the past decade or more, much of cell biology research has been focused on determining the key molecules involved in different cellular processes, an analytical problem that has been amenable to biochemical and genetic approaches. Now, we face an integrative problem of understanding how all of these molecules work together to produce living cells, a challenge that requires using quantitative approaches to model the complex interactions within a cell, and testing those models with careful quantitative measurements. This book is an introductory overview of the various approaches, methods, techniques, and models employed in quantitative cell biology, which are reviewed in greater detail in the other volumes in this e-book series. Particular emphasis is placed on the goals and purpose of quantitative analysis and modeling, and the special challenges that cell biology holds for understanding life at the physical level.
Nanoscale Photonic Imaging
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Author : Tim Salditt
language : en
Publisher: Springer Nature
Release Date : 2020-06-09
Nanoscale Photonic Imaging written by Tim Salditt and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-09 with Science categories.
This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.
Single Molecule Cellular Biophysics
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Author : Mark C. Leake
language : en
Publisher: Cambridge University Press
Release Date : 2013-01-31
Single Molecule Cellular Biophysics written by Mark C. Leake and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-31 with Science categories.
Indispensable textbook for undergraduate students in the physical and life sciences, unravelling the inner workings of the cell.
Cellular And Molecular Renewal In The Mammalian Body
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Author : Ivan L. Cameron
language : en
Publisher: Academic Press
Release Date : 2013-10-22
Cellular And Molecular Renewal In The Mammalian Body written by Ivan L. Cameron and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-22 with Nature categories.
Cellular and Molecular Renewal in the Mammalian Body concerns the dynamic nature of body constituents at the molecular, organelle, and cellular level of structural organization. Each chapter of this book deals with cellular and molecular renewal, proliferation, and loss, focusing on the body's major macromolecular classes, such as DNA, RNA, proteins, lipids, and carbohydrates. Other topics discussed include the subcellular renewal, DNA stability, regulation of cell proliferation, and changes in mitotic rates. The intracellular sites of RNA synthesis, proteins of the nucleus, and epithelial-mesenchymal interactions are also elaborated. This text likewise covers the brown adipose tissue, histochemistry of polysaccharides, and calcification. This publication is beneficial to students and investigators intending to acquire knowledge of the dynamic nature of body constituents and its influence to the analysis and experimentation of normal, disease, and repair processes within the body.
Molecular Biology In Cellular Pathology
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Author : John Crocker
language : en
Publisher: John Wiley & Sons
Release Date : 2003-06-02
Molecular Biology In Cellular Pathology written by John Crocker and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-06-02 with Science categories.
The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.
Physical Principles In Sensing And Signaling
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Author : Robert G. Endres
language : en
Publisher: Oxford University Press
Release Date : 2013-01-10
Physical Principles In Sensing And Signaling written by Robert G. Endres and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-10 with Science categories.
"This textbook is aimed at newcomers in the field of biological physics, especially motivated undergraduate and beginning graduate students with a mathematical and physical background, taking interest in the area of quantitative biology"--