Crispr Cas Methods
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Crispr Cas Methods
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Author : M. Tofazzal Islam
language : en
Publisher: Humana
Release Date : 2021-07-31
Crispr Cas Methods written by M. Tofazzal Islam and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-31 with Science categories.
This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Crispr Cas Methods
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Author : M. Tofazzal Islam
language : en
Publisher: Springer Nature
Release Date : 2025-03-19
Crispr Cas Methods written by M. Tofazzal Islam and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-19 with Science categories.
This third edition expands upon the previous two editions with new and updated methods presenting comprehensive, step-by-step protocols for recently developed techniques that were not included in the earlier volumes. Chapters feature contributions from the globally leading experts in the field, aiming to equip researchers with the practical tools necessary for successful CRISPR experimentation. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 3 aims to be a useful and practical guide for researches to help further their study in this field.
Crispr
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Author : Magnus Lundgren
language : en
Publisher: Humana
Release Date : 2015-05-20
Crispr written by Magnus Lundgren and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-20 with Medical categories.
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
Genome Engineering Via Crispr Cas9 System
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Author : Vijai Singh
language : en
Publisher: Academic Press
Release Date : 2020-02-21
Genome Engineering Via Crispr Cas9 System written by Vijai Singh and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-02-21 with Science categories.
Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.
Crispr Cas Systems
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Author : Rodolphe Barrangou
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-13
Crispr Cas Systems written by Rodolphe Barrangou and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-13 with Science categories.
CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.
Crispr Cas Enzymes
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Author :
language : en
Publisher: Academic Press
Release Date : 2019-01-25
Crispr Cas Enzymes written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-25 with Science categories.
CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Updated release includes the latest information on the CRISPR-Cas Enzymes
Introduction To Crispr Cas9 Techniques
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Author : Michael J. Wolyniak
language : en
Publisher: Springer Nature
Release Date : 2025-02-17
Introduction To Crispr Cas9 Techniques written by Michael J. Wolyniak and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-02-17 with Science categories.
This open-access textbook provides an in-depth introduction into the CRISPR-cas9 technology and explores its use across the gamut of biological model systems. As the subject has risen from a significant new discovery to a mainstream molecular biology practice, it is essential that students of molecular biology understand the fundamentals behind CRISPR-Cas9 technology and how it may be employed efficiently and ethically in research. This volume, edited by experts in both, molecular biology and undergraduate education, will teach not only the fundamentals of using CRISPR-Cas9, but also how to successfully employ this technology in classroom settings. The book is written for undergraduates and advanced high school classes in the area of molecular biology, genetics, genomics and biological engineering and will provide a perfect tool for undergraduate lecturers to prepare their classes.
Crispr Cas Methods
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Author : M. Tofazzal Islam
language : en
Publisher: Humana
Release Date : 2021-07-14
Crispr Cas Methods written by M. Tofazzal Islam and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-14 with Science categories.
This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
The Crispr Cas Tool Kit For Genome Editing
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Author : Aftab Ahmad
language : en
Publisher: Springer Nature
Release Date : 2022-01-01
The Crispr Cas Tool Kit For Genome Editing written by Aftab Ahmad and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-01 with Medical categories.
This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool. The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.
Crispr Cas Systems For Rna And Genome Editing
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Author :
language : en
Publisher: Elsevier
Release Date : 2025-03-25
Crispr Cas Systems For Rna And Genome Editing written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-25 with Science categories.
CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more.Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more. - Provides the latest information on DNA/RNA research - Offers outstanding and original reviews on a range of DNA/RNA research topics - Serves as an indispensable reference for researchers and students alike