Cryogenic Super Resolved Fluorescence Microscopy
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Cryogenic Super Resolved Fluorescence Microscopy
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Author : Siegfried Weisenburger
language : en
Publisher: Logos Verlag Berlin GmbH
Release Date : 2016-11-16
Cryogenic Super Resolved Fluorescence Microscopy written by Siegfried Weisenburger and has been published by Logos Verlag Berlin GmbH this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-16 with Science categories.
The significance of super-resolved fluorescence microscopy beyond the diffraction barrier was recognized by the Nobel Prize in Chemistry in 2014. At room temperature, these techniques typically achieve a resolution on the order of twenty nanometers. They already allowed for resolving subcellular structures and organelles, and are starting to enable discoveries in neuroscience, molecular biology and other life sciences. One can dream about increasing the optical resolution by another two orders of magnitude in order to directly resolve sub-molecular structures such as constituents of molecular complexes or even protein structure itself. The aim of the present work is to accomplish exactly that. In this PhD thesis, a novel microscopy technique is presented that exploits cryogenic measurements to push optical resolution to the Angstrom level. The near atomic resolution is made possible by the substantial improvement of the molecular photostability at liquid helium temperature. This method allows one to gain structural information of proteins or other molecular complexes that might not be accessible by existing analytical methods such as X-ray crystallography, cryogenic electron microscopy or magnetic resonance spectroscopy. These results mark record optical resolution and demonstrate that optical resolution can be pushed beyond the diffraction limit by nearly one thousand times.
Correlative Light And Electron Microscopy Iv
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Author :
language : en
Publisher: Academic Press
Release Date : 2021-03-09
Correlative Light And Electron Microscopy Iv written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-03-09 with Science categories.
Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. Contains contributions from experts in the field Covers topics using nano-SIMS and EDX for CLEM Presents recent advances and currently applied correlative approaches Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting Covers CLEM approaches in the context of specific applications Aims to stimulate the use of new combinations of imaging modalities
Imaging In Virus Research
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Author :
language : en
Publisher: Elsevier
Release Date : 2023-07-29
Imaging In Virus Research written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-07-29 with Science categories.
Approx.250 pages Approx.250 pages
Correlative Light And Electron Microscopy V
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Author : Thomas Müller-Reichert
language : en
Publisher: Elsevier
Release Date : 2024-05-04
Correlative Light And Electron Microscopy V written by Thomas Müller-Reichert and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-05-04 with Science categories.
Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Cellular Imaging
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Author : Eric Hanssen
language : en
Publisher: Springer
Release Date : 2017-12-30
Cellular Imaging written by Eric Hanssen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-30 with Science categories.
This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Dna Nanotechnology For Cell Research
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Author : Zhou Nie
language : en
Publisher: John Wiley & Sons
Release Date : 2024-02-13
Dna Nanotechnology For Cell Research written by Zhou Nie and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-02-13 with Science categories.
DNA Nanotechnology for Cell Research Comprehensive coverage of DNA nanotechnology with a focus on its biomedical applications in disease diagnosis, gene therapy, and drug delivery Bringing together multidisciplinary aspects of chemical, material, and biological engineering, DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine presents an overview of DNA nanotechnology with emphasis on a variety of different applications in cell research and engineering, covering a unique collection of DNA nanotechnology for fundamental research and engineering of living cells, mostly in cellulo and in vivo, for the first time. Broad coverage of this book ranges from pioneering concepts of DNA nanotechnology to cutting-edge reports regarding the use of DNA nanotechnology for fundamental cell science and related biomedical engineering applications in sensing, bioimaging, cell manipulation, gene therapy, and drug delivery. The text is divided into four parts. Part I surveys the progress of functional DNA nanotechnology tools for cellular recognition. Part II illustrates the use of DNA-based biochemical sensors to monitor and image intracellular molecules and processes. Part III examines the use of DNA to regulate biological functions of individual cells. Part IV elucidates the use of DNA nanotechnology for cell-targeted medical applications. Sample topics covered in DNA Nanotechnology for Cell Research include: Selections and applications of functional nucleic acid toolkits, including DNA/RNA aptamers, DNAzymes, and riboswitches, for cellular recognition, metabolite detection, and liquid biopsy. Developing intelligent DNA nanodevices implemented in living cells for amplified cell imaging, smart intracellular sensing, and in cellulo programmable biocomputing. Harnessing dynamic DNA nanotechnology for non-genetic cell membrane engineering, receptor signaling reprogramming, and cellular behavior regulation. Construction of biocompatible nucleic acid nanostructures as precisely controlled vehicles for drug delivery, immunotherapy, and tissue engineering. Providing an up-to-date tutorial style overview along with a highly valuable in-depth perspective, DNA Nanotechnology for Cell Research is an essential resource for the entire DNA-based nanotechnology community, including analytical chemists, biochemists, materials scientists, and bioengineers.
Cryo Electron Tomography
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Author : Friedrich Förster
language : en
Publisher: Springer Nature
Release Date :
Cryo Electron Tomography written by Friedrich Förster and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Fluorescence Microscopy
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Author : Anda Cornea
language : en
Publisher: Elsevier
Release Date : 2014-02-24
Fluorescence Microscopy written by Anda Cornea and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-24 with Science categories.
Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions
Recent Advances In Fluorescent Probes For Super Resolution Microscopy
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Author : Chayan Kanti Nandi
language : en
Publisher: Frontiers Media SA
Release Date : 2021-07-21
Recent Advances In Fluorescent Probes For Super Resolution Microscopy written by Chayan Kanti Nandi and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-21 with Science categories.
Biological Field Emission Scanning Electron Microscopy
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Author : Roland A. Fleck
language : en
Publisher: John Wiley & Sons
Release Date : 2019-02-06
Biological Field Emission Scanning Electron Microscopy written by Roland A. Fleck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-06 with Science categories.
The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.