Cryopreservation And Freeze Drying Protocols Methods In Molecular Biology
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Cryopreservation And Freeze Drying Protocols
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Author : John G. Day
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-06-05
Cryopreservation And Freeze Drying Protocols written by John G. Day and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-06-05 with Science categories.
In addition to outlining the fundamental principles associated with the conservation of biological resources, freeze-drying and cryopreservation, this text is a compilation of cryptopreservation and freeze-drying methodologies applicable to different biological materiels, developed by expert laboratories.
Cryopreservation And Freeze Drying Protocols
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Author : Willem F. Wolkers
language : en
Publisher: Springer
Release Date : 2014-11-27
Cryopreservation And Freeze Drying Protocols written by Willem F. Wolkers and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-27 with Science categories.
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
Cryopreservation And Freeze Drying Protocols Methods In Molecular Biology
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Author : John G. Day
language : en
Publisher:
Release Date : 2007
Cryopreservation And Freeze Drying Protocols Methods In Molecular Biology written by John G. Day and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
This book, in addition to outlining the fundamental principles associated with the conservation of Biological Resources, freeze-drying and cryopreservation, is a compilation of cryopreservation and freeze-drying methodologies applicable to different biological materials, which have been developed by expert laboratories. The protocols are reproducible, robust, and most have been transferred successfully to other laboratories. "Cryopreservation and Freeze-Drying Protocols, Second Edition" expands on the range of materials previously covered and includes many novel approaches and protocols for biological materials that were not preservable at the time.
Cryopreservation And Freeze Drying Protocols Methods In Molecular Biology
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Author : John G. Day
language : en
Publisher:
Release Date : 1995
Cryopreservation And Freeze Drying Protocols Methods In Molecular Biology written by John G. Day and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials. Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections. Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.
Pollen Cryopreservation Protocols
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Author : P.E. Rajasekharan
language : en
Publisher: Springer Nature
Release Date : 2023-02-20
Pollen Cryopreservation Protocols written by P.E. Rajasekharan and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-20 with Science categories.
This volume focuses upon pollen cryopreservation in various crops from the most widely cultivated tomato to the lesser known medicinal species like Decalepis arayalpatra. Chapters detail standard techniques such as pollen collection, cryopreservation, pollen , germination tests for viability, and specific crops. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs. Authoritative and cutting-edge, Pollen Cryopreservation Protocols aims to serve as reference for researchers studying plant breeding and plant conservation biology.
Practical Fermentation Technology
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Author : Brian McNeil
language : en
Publisher: John Wiley & Sons
Release Date : 2008-04-15
Practical Fermentation Technology written by Brian McNeil and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-15 with Science categories.
A hands-on book which begins by setting the context;- defining 'fermentation' and the possible uses of fermenters, and setting the scope for the book. It then proceeds in a methodical manner to cover the equipment for research scale fermentation labs, the different types of fermenters available, their uses and modes of operation. Once the lab is equipped, the issues of fermentation media, preservation strains and strain improvement strategies are documented, along with the use of mathematical modelling as a method for prediction and control. Broader questions such as scale-up and scale down, process monitoring and data logging and acquisition are discussed before separate chapters on animal cell culture systems and plant cell culture systems. The final chapter documents the way forward for fermenters and how they can be used for non-manufacturing purposes. A glossary of terms at the back of the book (along with a subject index) will prove invaluable for quick reference.Edited by academic consultants who have years of experience in fermentation technology, each chapter is authored by experts from both industry and academia. Industry authors come from GSK (UK), DSM (Netherlands), Eli Lilly (USA) and Broadley James (UK-USA).
Plant Cryopreservation A Practical Guide
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Author : Barbara B.M. Reed
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-31
Plant Cryopreservation A Practical Guide written by Barbara B.M. Reed and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-31 with Technology & Engineering categories.
Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Crop Improvement
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Author : Pankaj Kumar
language : en
Publisher: CRC Press
Release Date : 2021-06-29
Crop Improvement written by Pankaj Kumar and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-06-29 with Science categories.
Crop Improvement: Biotechnological Advances – Biomedical Science The field of biotechnology is advancing at a fast pace. The availability of low-cost DNA/genome sequencing technologies has led to the discovery and functional characterization of myriad of genes imparting stress tolerance and quality traits. The ‘omics’ group of technologies including genomics, proteomics, transcriptomics and metabolomics has revolutionized the agricultural biotechnology sector. The Nobel Prize-winning technology, such as the genome editing technique, is being employed to edit various gene functions in plants aiding in crop improvement. This technology may be adopted very quickly by consumers compared with the transgenic technique because the genome-edited plants have no adverse effects on the genome of the plant itself and on the environment and related species/non-target organisms. In this book, authors have attempted to compile the latest techniques of agricultural biotechnology and their applications in crop improvement. Certain chapters have been dedicated to describe the use of nanotechnology, a fast emerging new technique in the agriculture sector. Features Development, potential and safety issues in biotechnology Advances in genomics, proteomics and transcriptomics in agriculture Protein bioinformatics and its applications Genetically modified (GM) technology and its implications Genome editing in crop improvement Marker-assisted selection (MAS) in crop improvement Mutation breeding Cryobiotechnology Nanotechnology and biosensors This book includes real-world examples and applications making it accessible to a broader interdisciplinary readership. We hope that it will serve as a reference book for researchers engaged in molecular biology and biotechnology and will act as a ready reckoner for postgraduate (PG) students in the biotechnology discipline.
Cryobanking The Genetic Resource
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Author : Paul Watson
language : en
Publisher: CRC Press
Release Date : 2001-02-16
Cryobanking The Genetic Resource written by Paul Watson and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-02-16 with Nature categories.
There is a growing awareness of a worldwide reduction in biodiversity and the urgent need to develop ways to redress the problem. This is the first major book devoted to the subject of genetic resource banking (GRB) and its role in preserving global animal biodiversity. In Cryobanking the Genetic Resource, expert contributors provide the non-specialist with an overview of the subject and the practical techniques associated with GRB. The book presents a basic introduction to the concepts, and then points the way to relevant literature for those who wish to develop practical applications. The first section deals with the potential contribution of GRB to biodiversity protection, while the second section offers an introduction to the basic cryobiology of gametes and embryos. In the first section, genetic considerations for planning interventions are discussed, together with disease control measures. This section explores related reproductive technologies necessary for the use of cryopreserved gametes and embryos, as well as a range of other issues such as ownership and security of stored material, and the size and location of cryopreserved material. The second section offers a thorough review of the literature on cryopreservation of spermatozoa, oocytes, and embryos. It gathers species into related groups for ease of reference, and does not assume extensive specialist knowledge so that newcomers to the field can make the best use of the available research. This book is an essential resource for zoologists and conservationists with an interest in genetic management and captive breeding, and also for students involved in biodiversity issues, conservation, and assisted reproduction.
Plant Conservation Biotechnology
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Author : Dr Erica Benson
language : en
Publisher: CRC Press
Release Date : 2002-04-12
Plant Conservation Biotechnology written by Dr Erica Benson and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-04-12 with Science categories.
Introduces biotechnological techniques which are currently used to conserve horticultural and crop plant germplasm, forest tree genetic resources, endangered plant species, and plant cell culture collections. Covers techniques and applications.