Crystallization Of Nucleic Acids And Proteins
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Crystallization Of Nucleic Acids And Proteins
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Author : Arnaud Ducruix
language : en
Publisher: OUP Oxford
Release Date : 1999-10-21
Crystallization Of Nucleic Acids And Proteins written by Arnaud Ducruix and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-10-21 with Science categories.
Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucliec Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.
Crystallization Of Nucleic Acids And Proteins
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Author : Arnaud Ducruix
language : en
Publisher:
Release Date : 1999
Crystallization Of Nucleic Acids And Proteins written by Arnaud Ducruix and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Science categories.
Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucleic Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucleic Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.
Crystallization Of Nucleic Acids And Proteins
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Author : Arnaud Ducruix
language : en
Publisher: Oxford University Press, USA
Release Date : 1992
Crystallization Of Nucleic Acids And Proteins written by Arnaud Ducruix and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Language Arts & Disciplines categories.
Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the firstedition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology(maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films.As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminarycharacterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocolsto provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucliec Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology,crystallography, protein engineering, and biological chemistry.
Crystallization Of Nucleic Acids And Proteins
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Author : A. Ducruix
language : en
Publisher:
Release Date :
Crystallization Of Nucleic Acids And Proteins written by A. Ducruix and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with Crystallization categories.
This volume offers a laboratory guide to the "black out" of crystallization, providing a detailed guide to producing crystals of proteins and nucleic acids for diffraction studies, necessary for biomedical research.
Handbook Of Industrial Crystallization
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Author : Allan Myerson
language : en
Publisher: Butterworth-Heinemann
Release Date : 2002-01-08
Handbook Of Industrial Crystallization written by Allan Myerson and has been published by Butterworth-Heinemann this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-01-08 with Technology & Engineering categories.
Crystallization is an important separation and purification process used in industries ranging from bulk commodity chemicals to specialty chemicals and pharmaceuticals. In recent years, a number of environmental applications have also come to rely on crystallization in waste treatment and recycling processes.The authors provide an introduction to the field of newcomers and a reference to those involved in the various aspects of industrial crystallization. It is a complete volume covering all aspects of industrial crystallization, including material related to both fundamentals and applications. This new edition presents detailed material on crystallization of biomolecules, precipitation, impurity-crystal interactions, solubility, and design.Provides an ideal introduction for industrial crystallization newcomers Serves as a worthwhile reference to anyone involved in the fieldCovers all aspects of industrial crystallization in a single, complete volume
Protein Crystallization Strategies For Structural Genomics
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Author : Naomi E. Chayen
language : en
Publisher: Internat'l University Line
Release Date : 2007
Protein Crystallization Strategies For Structural Genomics written by Naomi E. Chayen and has been published by Internat'l University Line this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Science categories.
Macromolecular Crystallography
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Author : Mark R. Sanderson
language : en
Publisher: Oxford University Press
Release Date : 2007-08-23
Macromolecular Crystallography written by Mark R. Sanderson and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-23 with Language Arts & Disciplines categories.
Macromolecular crystallography is the study of macromolecules using X-ray crystallographic techniques to determine their molecular structure. This title synthesises contributions from a team of internationally recognized leaders, offering chapters on conventional and high-throughput methods.
Crystallization Of Membrane Proteins
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Author : Hartmut Michel
language : en
Publisher: CRC Press
Release Date : 2018-01-18
Crystallization Of Membrane Proteins written by Hartmut Michel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-18 with Medical categories.
The precise knowledge of the structure of biological macromolecules forms the basis of understanding their function and their mechanism of action. It also lays the foundation for rational protein and drug design. The only method to obtain this knowledge is still crystallography. At present, the structures of about 400 proteins are known at or nearly at atomic proteins. However, only two of them are membrane proteins or complexes of the membrane proteins. The reasons for the difference is not the crystals of membrane proteins resists forming special problems when being analysed. The reason is that the membrane proteins resist into forming into well-ordered crystals. The intention of this book is to help to produce well-ordered crystals proteins and to provide guidelines, it is aimed at both biochemists and protein crystallographer‘s.
Principles Of Nucleic Acid Structure
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Author : Stephen Neidle
language : en
Publisher: Elsevier
Release Date : 2010-07-26
Principles Of Nucleic Acid Structure written by Stephen Neidle and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-26 with Science categories.
This unique and practical resource provides the most complete and concise summary of underlying principles and approaches to studying nucleic acid structure, including discussion of x-ray crystallography, NMR, molecular modelling, and databases. Its focus is on a survey of structures especially important for biomedical research and pharmacological applications. To aid novices, Principles of Nucleic Acid Structure includes an introduction to technical lingo used to describe nucleic acid structure and conformations (roll, slide, twist, buckle, etc.). This completely updated edition features expanded coverage of the latest advances relevant to recognition of DNA and RNA by small molecules and proteins. In particular, the reader will find extensive new discussions on: RNA folding, ribosome structure and antibiotic interactions, DNA quadruplexes, DNA and RNA protein complexes, and short interfering RNA (siRNA). This handy guide ends with a complete list of resources, including relevant online databases and software. - Completely updated with expanded discussion of topics such as RNA folding, ribosome structure and antibiotic interactions, DNA quadruplexes, DNA and RNA protein complexes, and short interfering RNA (siRNA) - Includes a complete list of resources, including relevant online databases and software - Defines technical lingo for novices