Culture Of Human Tumor Cells
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Culture Of Human Tumor Cells
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Author : Roswitha Pfragner
language : en
Publisher: John Wiley & Sons
Release Date : 2005-03-11
Culture Of Human Tumor Cells written by Roswitha Pfragner and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-03-11 with Science categories.
Ein neuer Band aus der 'Culture of Specialized Cells'-Reihe. Leserfreundlich aufgemacht. Er vermittelt spezifische praktische Details, wie man Medien und Reagenzien sowie Protokolle für Zellisolierung und Zellkultur präpariert. Logisch aufgebaut und nach spezifischen Tumoren gegliedert. Farbtafeln demonstrieren anschaulich Immunozytochemie und Fluoreszenz in situ Hybridisierung (FISH). Darüber hinaus beschreibt das Buch auch umfangreiche Sicherheitsvorkehrungen. Mit einer Vielzahl nützlicher Tipps. Mit einem Glossar zu ausgewählten Fachtermini. Enthält eine umfangreiche Liste mit Bezugsadressen von Ausrüstung und Zellkulturprodukten. Erläutert medikamentöse Behandlung, Auswahl, Differenzierung, Assays für die Untersuchung maligner Zellen sowie Risiken und Anwendungsmöglichkeiten.
Human Tumor Cells In Vitro
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Author : Jorgen Fogh
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Human Tumor Cells In Vitro written by Jorgen Fogh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Medical categories.
The study of cultured human tumor cells is a most obvious approach in experimental human cancer research. For many techniques in virology, immunology, biochemistry, and biophysics, for example, large amounts of cells may be required and such quantities are usually provided only when the cultures develop into established cell lines; when this happens, thorough characterization also becomes possible. The development of cell lines, therefore, is of prime importance. Recent major advances in research with animal cell systems see m to be a prologue for present and future efforts directed toward work with human tumor cells in culture. Conceivably, the most significant results in cancer research may develop from work with such cells, and so the time seemed right to define the present state of our knowledge. This is the first book dedicated exclusively to the subject: human tumor cells in vitro. Although so me of the fundamental aspects in the cultivation of human tumor ceIls, and the extent to which they represent human cancer in vivo are still unclear, I asked a number of the leading investigators in this area of research to collect and evaluate previous and present contributions, and to offer their thoughts on the questions to which answers are not yet available. Many of the chapters are concerned with techniques of cultivation. Cultures from some types of tumors have grown weIl; in many cases they have given rise to established cell lines.
Cancer Cell Lines Part 1
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Author : John Masters
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-04-11
Cancer Cell Lines Part 1 written by John Masters and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-04-11 with Medical categories.
Continuous cell lines derived from human cancers are the most widely used resource in laboratory-based cancer research. The first 3 volumes of this series on Human Cell Culture are devoted to these cancer cell lines. The chapters in these first 3 volumes have a common aim. Their purpose is to address 3 questions of fundamental importance to the relevance of human cancer cell lines as model systems of each type of cancer: 1. Do the cell lines available accurately represent the clinical presentation? 2. Do the cell lines accurately represent the histopathology of the original tumors? 3. Do the cell lines accurately represent the molecular genetics of this type of cancer? The cancer cell lines available are derived, in most cases, from the more aggressive and advanced cancers. There are few cell lines derived from low grade organ-confined cancers. This gap can be filled with conditionally immortalized human cancer cell lines. We do not know why the success rate for establishing cell lines is so low for some types of cancer and so high for others. The histopathology of the tumor of origin and the extent to which the derived cell line retains the differentiated features of that tumor are critical. The concept that a single cell line derived from a tumor at a particular site is representative of tumors at that site is naïve and misleading.
Atlas Of Human Tumor Cell Lines
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Author : Robert K.M. Hay
language : en
Publisher: Academic Press
Release Date : 2013-10-22
Atlas Of Human Tumor Cell Lines written by Robert K.M. Hay and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-22 with Medical categories.
Human tumor cells in culture are valuable for studying cancer causes and properties. This convenient reference provides useful information for cancer researchers on commonly used, established tumor cell lines of the major human organ systems. Atlas of Human Tumor Cell Lines includes data about morphological, metabolic, genetic, and growth characteristics of human tumor cells, with morphological characteristics presented in more than 250 photomicrographs. It also contains information for establishing and maintaining human tumor cell lines in culture, and each chapter covers future perspectives. Covers well-characterized tumor cell lines from the major human organ systems Presents over 250 photomicrographs, both phase-contrast and electron micrographs Includes a list of key references for each chapter Written by world-renowned experts
Cultural Of Human Tumor Cells Cultural Of Epithelial Cells 2e Set
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Author : Roswitha Pfragner
language : en
Publisher: Wiley-Liss
Release Date : 2003-11-20
Cultural Of Human Tumor Cells Cultural Of Epithelial Cells 2e Set written by Roswitha Pfragner and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-20 with Medical categories.
2 Volumne set includes ISBN: 9780471401216 Culture of Epithelial Cells, 2nd Edition and ISBN: 9780471438533 Culture of Human Tumor Cells.
Human Cancer In Primary Culture A Handbook
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Author : John Masters
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Human Cancer In Primary Culture A Handbook written by John Masters and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Growing human cancer cells in primary culture requires patience, intuition, care and experience. This is one of the few areas where the wrinkled senior scientist can be more productive than the bright young post-doc. There are few mechanical aids, no automated procedures, and kits are unheard-of. There is no right way to do it and every tumour is different. But this book will make it easier! Chapter 1 on characterization is essential reading. Much published work is useless because of the failure to take two simple steps to characterise the cells. The first step is to fully record all the clinical data - the absence of this information can render the work valueless. The second step is to confirm the origin of the tissue to exclude cross contamination. The wastage of years of work can be avoided with the use of a simple DNA preparation with a couple of commercially-available probes. Chapter 2 describes the development of serum-free media. This is a goal many would like to achieve, particularly if someone else does it, as it is laborious and empirical. Defined serum-free medium is essential for studies of growth factors and has major advantages in the commercial preparation of cell products and other applications.
Cancer Cell Lines
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Author : Bernhard Ø Palsson
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-04-11
Cancer Cell Lines written by Bernhard Ø Palsson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-04-11 with Medical categories.
This book describes all human leukemia-lymphoma cell lines that have been established and that grow continuously under standardised in vitro conditions. These lines are derived from cells belonging to all the major hematopoietic cell lineages, i.e. B- and T-lymphocytes, natural killer cells, granulocytic cells and megakaryocytic cells. The clinical data, the culture conditions and the major phenotypic features of the cell lines are described with citations. This book is the first book describing human leukemia-lymphoma cell lines and will be of interest to scientists involved in the areas of hematology, oncology, immunology, molecular biology and cytogenetics. Cancer Cell Lines, Volumes 1-3: These 3 volumes provide a comprehensive text on the culture of established cell lines from every type of human cancer. The volumes provide a basic manual and reference resource for every cancer research scientist using human cancer cells.
Cancer Cell Lines Part 2
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Author : John Masters
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-04-11
Cancer Cell Lines Part 2 written by John Masters and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-04-11 with Medical categories.
Continuous cell lines derived from human cancers are the mostwidely used resource in laboratory-based cancer research. The first 3 volumes of this series on Human Cell Culture are devoted to these cancer cell lines. The chapters in these first 3 volumes have a common aim. Their purpose is to address 3 questions offundamental importance to the relevanceof human cancer cell lines as model systems of each type of cancer: 1. Do the cell lines available accurately represent the clinical presentation? 2. Do the cell lines accurately represent the histopathology of the original tumors? 3. Do the cell lines accurately represent the molecular genetics of this type of cancer? The cancer cell lines available are derived, in most cases, from the more aggressive and advanced cancers. There are few cell lines derived from low grade organ-confined cancers. This gap can be filled with conditionally immortalized human cancer cell lines. We do not know why the success rate for establishing cell lines is so low for some types of cancer and so high for others. The histopathology of the tumor of origin and the extent to which the derived cell line retains the differentiated features of that tumor are critical. The concept that a single cell line derived from a tumor at a particular site is representative oftumors at that site is naïve and misleading.
Culture Of Human Tumor Cells
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Author : Roswitha Pfragner
language : en
Publisher: Wiley-Liss
Release Date : 2003-11-21
Culture Of Human Tumor Cells written by Roswitha Pfragner and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-21 with Science categories.
This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.
Cancer Cell Culture
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Author : Simon P. Langdon
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Cancer Cell Culture written by Simon P. Langdon and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Medical categories.
The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.