Current Human Islet Isolation Protocl
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Current Human Islet Isolation Protocl
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Author : Jonathan R. T. Lakey
language : en
Publisher:
Release Date : 2004-04
Current Human Islet Isolation Protocl written by Jonathan R. T. Lakey and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-04 with Medical categories.
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Author :
language : en
Publisher:
Release Date : 1681
written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1681 with categories.
Islets Of Langerhans 2 Ed
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Author : Shahidul Islam
language : en
Publisher:
Release Date :
Islets Of Langerhans 2 Ed written by Shahidul Islam and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with Bioorganic chemistry categories.
Cryopreservation
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Author : Marian Quain
language : en
Publisher: BoD – Books on Demand
Release Date : 2020-06-10
Cryopreservation written by Marian Quain and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-10 with Medical categories.
Cryopreservation - Current Advances and Evaluations sheds light on storage of cells at subzero temperatures while ensuring that biological functionality is not compromised. Cryopreservation presents a perfect technique by which life can be preserved for posterity. However, there are many challenges to overcome and questions to answer, such as: Are organisms and metabolic systems functioning normally after cooling and thawing? This book provides comprehensive information on cryopreservation with a particular focus on cryoprotectant agents (CPAs). CPAs prevent ice from forming on cryogenically preserved cells, tissues, and organs, but can become toxic at high concentrations. As such, more research is needed to determine their precise mechanisms of action and to develop potential new CPAs that will not compromise the biology of cells. This book is an attempt in this direction.
Human Cell Culture Protocols
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Author : Ragai R. Mitry
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Human Cell Culture Protocols written by Ragai R. Mitry and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.
Pancreas And Islet Transplantation
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Author : Nadey S. Hakim
language : en
Publisher:
Release Date : 2002
Pancreas And Islet Transplantation written by Nadey S. Hakim and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.
Diabetes Mellitus is the principle cause of kidney failure and blindness in adults and leads to more cases of amputation and impotence than any other disease. It is one of the most common chronic diseases in childhood. The aims of pancreas or islet transplantation are to improve the quality of life of patients with insulin dependent diabetes mellitus and to ameliorate secondary complications. This book provides a comprehensive and international review of the recent advances in pancreas and islet transplantation. It covers surgical techniques, issues surrounding organ preservation, immunosuppression and the control of other complications, all of which contribute to the potential for such transplantations to evolve as the treatment of choice for insulin dependent diabetes. The editors have compiled a strong and international team of contribution authors. This book is essential reading for transplant surgeons and all those involved in researching or treating diabetes mellitus.
Pancreatic Islet Cell Transplantation
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Author : Camillo Ricordi
language : en
Publisher: Landes Bioscience
Release Date : 1992
Pancreatic Islet Cell Transplantation written by Camillo Ricordi and has been published by Landes Bioscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.
Intended for researchers and clinicians who work with cell transplantation, this text discusses the latest methods and gives an overall update of the major fields of islet transplantation. Included are reports of recent clinical trials on successful islet transplantation in humans.
Islet Transplantation And Beta Cell Replacement Therapy
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Author : A. M. James Shapiro
language : en
Publisher: CRC Press
Release Date : 2007-07-25
Islet Transplantation And Beta Cell Replacement Therapy written by A. M. James Shapiro and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-25 with Medical categories.
Beta cell replacement through transplantation remains the only treatment option for Type 1 diabetes enabling restoration of near-physiological glucose levels without significant hypoglycemia. Outlining the most recent advances and research breakthroughs, this practical guide and reference work explores the impact of islet cell transplantation and b
Pancreatic Islet Isolation
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Author : Miriam Ramírez-Domínguez
language : en
Publisher: Springer
Release Date : 2016-09-01
Pancreatic Islet Isolation written by Miriam Ramírez-Domínguez and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-01 with Science categories.
Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, access to high quality preparations of pancreatic islets is fundamental for in vitro studies and to test pre-clinical applications in animal models in vivo. Access to healthy human islets is also crucial to improve transplantation procedures for diabetes. Given the susceptibility of pancreatic islets to the enzymatic digestion and mechanical stress required to obtain them, the isolation of islets is often considered as the delicate “work of a craftsman”. This book, which is aimed at beginners and experts alike, is a survey of the current state-of–the-art in this field and it centres on the challenges, pitfalls and peculiarities of pancreatic islet isolation in the different species used in pre-clinical and clinical applications. It explores the similarities and differences between human islets and those from other relevant species (rodents, pigs and non-human primates), and how these influence islet isolation. The ultimate goal of this book is to improve the outcome of islet isolation and transplantation in pre-clinical and clinical applications.
Cell Microencapsulation
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Author : Emmanuel C. Opara
language : en
Publisher: Humana
Release Date : 2016-10-14
Cell Microencapsulation written by Emmanuel C. Opara and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-14 with Science categories.
This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in cell microencapsulation, the challenges to the technology, and the current status of its application in different clinical situations. This book is divided in five sections: Section I is an introductory part that discusses historical developments of the technology and its current challenges, as well as the various applications of cell microencapsulation; Section II discusses the main approaches and devices currently used in cell microencapsulation; Section III presents an overview of the various polymeric materials currently in use for cell microencapsulation and the enabling technologies to either monitor or enhance encapsulated cell function; Section IV gives specific examples of the methods used to encapsulate various cell types; and Section V provides an overview of the different clinical situations in which cell microencapsulation has been applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Cell Microencapsulation: Methods and Protocols is a valuable reference for researchers, engineers, clinicians, and other healthcare professionals, as well as food technologists who will find detailed descriptions of methods for the microencapsulation of specific cell types and their current of potential clinical and industrial applications. This volume also includes detailed information about the design and manufacture of different devices including large-scale production devices for use in cell microencapsulation.