Current Protocols In Nucleic Acid Chemistry

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Current Protocols In Nucleic Acid Chemistry

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Author : Serge L. Beaucage
language : en
Publisher: Current Protocols
Release Date : 2000

Current Protocols In Nucleic Acid Chemistry written by Serge L. Beaucage and has been published by Current Protocols this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Science categories.

Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of oligonucleotides, synthesis without base protection, and synthesis on microarrays. More specialized synthetic methods include synthesis of biologically active nucleosides and prodrugs. Purification and characterization methods are detailed. Advanced methods include biophysical analysis, combinatorial methods, and nanotechnology. Each protocol includes rationale for choosing appropriate methods, step-by-step procedures, complete recipes, anticipated results, characterization data, and troubleshooting, as well as background and recommended reading. The level of procedural detail is far beyond that found in the research literature, and tips and comments from authors are geared towards ensuring reliable duplication in the laboratory.

Nucleic Acids In Chemistry And Biology

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Author : G Michael Blackburn
language : en
Publisher: Royal Society of Chemistry
Release Date : 2015-11-09

Nucleic Acids In Chemistry And Biology written by G Michael Blackburn and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-11-09 with Science categories.

The structure, function and reactions of nucleic acids are central to molecular biology and are crucial for the understanding of complex biological processes involved. Revised and updated Nucleic Acids in Chemistry and Biology 3rd Edition discusses in detail, both the chemistry and biology of nucleic acids and brings RNA into parity with DNA. Written by leading experts, with extensive teaching experience, this new edition provides some updated and expanded coverage of nucleic acid chemistry, reactions and interactions with proteins and drugs. A brief history of the discovery of nucleic acids is followed by a molecularly based introduction to the structure and biological roles of DNA and RNA. Key chapters are devoted to the chemical synthesis of nucleosides and nucleotides, oligonucleotides and their analogues and to analytical techniques applied to nucleic acids. The text is supported by an extensive list of references, making it a definitive reference source. This authoritative book presents topics in an integrated manner and readable style. It is ideal for graduate and undergraduates students of chemistry and biochemistry, as well as new researchers to the field.

Current Protocols In Nucleic Acid Chemistry

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Author : Serge L. Beaucage
language : en
Publisher:
Release Date : 2001

Current Protocols In Nucleic Acid Chemistry written by Serge L. Beaucage and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.

Current Protocols In Nucleic Acid Chemistry

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Author : Serge L. Beaucage
language : en
Publisher:
Release Date : 199?

Current Protocols In Nucleic Acid Chemistry written by Serge L. Beaucage and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 199? with categories.

Current Protocols In Chemical Biology

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Author : Adam P. Arkin
language : en
Publisher: Wiley-Blackwell
Release Date : 2009-09-22

Current Protocols In Chemical Biology written by Adam P. Arkin and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-22 with categories.

Current Protocols In Nucleic Acid Chemistry Online

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Author : Bergstrom
language : en
Publisher: Current Protocols
Release Date : 2003-04-16

Current Protocols In Nucleic Acid Chemistry Online written by Bergstrom and has been published by Current Protocols this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-04-16 with categories.

Current Protocols Essential Laboratory Techniques

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Author : Sean R. Gallagher
language : en
Publisher: John Wiley & Sons
Release Date : 2012-03-19

Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-19 with Science categories.

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Current Protocols In Nucleic Acid Chemistry Supplement

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Author : Beaucage
language : en
Publisher:
Release Date : 2002-10-01

Current Protocols In Nucleic Acid Chemistry Supplement written by Beaucage and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-10-01 with categories.

Current Protocols In Nucleic Acid Chemistry Core Reprint

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Author : Beaucage
language : en
Publisher:
Release Date : 2002-10-01

Current Protocols In Nucleic Acid Chemistry Core Reprint written by Beaucage and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-10-01 with categories.

Current Protocols In Immunology

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Author : John E. Coligan
language : en
Publisher: Current Protocols
Release Date : 1991

Current Protocols In Immunology written by John E. Coligan and has been published by Current Protocols this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Medical categories.

Current Protocols in Immunology is a three-volume looseleaf manual that provides comprehensive coverage of immunological methods from classic to the most cutting edge, including antibody detection and preparation, assays for functional activities of mouse and human cells involved in immune responses, assays for cytokines and their receptors, isolation and analysis of proteins and peptides, biochemistry of cell activation, molecular immunology, and animal models of autoimmune and inflammatory diseases. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Bimonthly updates, which are filed into the looseleaf, keep the set current with the latest developments in immunology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.