Designing Antibodies
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Designing Antibodies
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Author : Ruth Mayforth
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Designing Antibodies written by Ruth Mayforth and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Medical categories.
Designing Antibodies provides a compilation of research in the design of antibodies. It describes the techniques used in antibody design, the kinds of antibodies generated through modern techniques, and their applications in medicine and science. The book begins with an overview of the humoral immune system and of antibody structure, function, and biosynthesis, which sets the stage for the subsequent discussions of developments in antibody technology. The remaining chapters discuss the making of monoclonal antibodies; the design of antibodies for human therapy; the connection of antibodies (either chemically or genetically) to other potentially therapeutic effector molecules such as toxins, enzymes, or even an antibody of another specificity; idiotypes and anti-idiotypic antibodies; and the ability of antibodies to functionally mimic enzymes and mediate catalysis. It is hoped that, in addition to illustrating the progress of research in antibody design, the various creative and innovative approaches reviewed in this book will be modified or will stimulate new ideas that will spur the research and application of designer antibodies.
Designing Antibodies
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Author : Ruth D. Mayforth
language : en
Publisher: Academic Press
Release Date : 1993-01-01
Designing Antibodies written by Ruth D. Mayforth and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-01-01 with Anti-idiotypic antibodies. categories.
Brings together the latest research of antibody design. Complete with illustrations, the text describes techniques, examines the types of new antibodies, and studies their applications in medicine and science. It is developed around strategies employed to design antibodies.
Computer Aided Antibody Design
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Author : Kouhei Tsumoto
language : en
Publisher: Springer Nature
Release Date : 2022-11-08
Computer Aided Antibody Design written by Kouhei Tsumoto and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-08 with Medical categories.
This volume details state-of-the- art methods on computer-aided antibody design. Chapters guide readers through information on antibody sequences and structures, modeling antibody structures and dynamics, prediction and optimization of biological and biophysical properties of antibodies, prediction of antibody-antigen interactions, and computer-aided antibody affinity maturation and beyond. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Computer-Aided Antibody Design aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. Chapter 2 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Development Of Antibody Based Therapeutics
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Author : Mohammad A. Tabrizi
language : en
Publisher: Springer
Release Date : 2014-05-08
Development Of Antibody Based Therapeutics written by Mohammad A. Tabrizi and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-08 with Medical categories.
Translational strategies for development of antibody-based therapeutics should allow understanding of the relationship between the ‘unit dose’ and ‘unit effect’ with respect to both beneficial and deleterious effects from early stages of development. The flow of information from later to earlier stages of development should provide opportunities to facilitate selection of more effective novel and next-generation drug candidates. Selection and evaluation of relevant biomarkers in early preclinical development in "relevant" animal models should allow for identifying potential risks to humans and establishing safe First-In-Human (FIH) dosing strategies. Hence, integration of knowledge with respect to target antigen properties such as antigen distribution, expression profile, kinetic properties, target pharmacology, antigen isoforms and pharmacological redundancy in health and disease, as well as antibody design criteria, such as antibody isotype, affinity, PK/PD and safety is a critical necessity for the design of effective translational strategies. Additionally, these factors will further offer critical differentiating characteristics for next-generation antibodies, and novel technologies prove instrumental in generation of biosuperior antibody candidates for market entry. This book will examine many important considerations necessary for the design of effective translational strategies during the development of antibody-based therapeutics.
Innovations For Next Generation Antibody Drug Conjugates
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Author : Marc Damelin
language : en
Publisher: Springer
Release Date : 2018-05-29
Innovations For Next Generation Antibody Drug Conjugates written by Marc Damelin and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-29 with Medical categories.
Antibody-drug conjugates (ADCs) stand at the verge of a transformation. Scores of clinical programs have yielded only a few regulatory approvals, but a wave of technological innovation now empowers us to overcome past technical challenges. This volume focuses on the next generation of ADCs and the innovations that will enable them. The book inspires the future by integrating the field’s history with novel strategies and cutting-edge technologies. While the book primarily addresses ADCs for solid tumors, the last chapter explores the emerging interest in using ADCs to treat other diseases. The therapeutic rationale of ADCs is strong: to direct small molecules to the desired site of action (and away from normal tissues) by conjugation to antibodies or other targeting moieties. However, the combination of small and large molecules imposes deep complexity to lead optimization, pharmacokinetics, toxicology, analytics and manufacturing. The field has made significant advances in all of these areas by improving target selection, ADC design, manufacturing methods and clinical strategies. These innovations will inspire and educate scientists who are designing next-generation ADCs with the potential to transform the lives of patients.
A Practical Guide To Monoclonal Antibodies
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Author : J. Eryl Liddell
language : en
Publisher: John Wiley & Sons
Release Date : 1991-08-26
A Practical Guide To Monoclonal Antibodies written by J. Eryl Liddell and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-08-26 with Medical categories.
Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.
Antibody Engineering
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Author : Benny K. C. Lo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Antibody Engineering written by Benny K. C. Lo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The exquisite binding specificity of antibodies has made them valuable tools from the laboratory to the clinic. Since the description of the murine hybridoma technology by Köhler and Milstein in 1975, a phenomenal number of mo- clonal antibodies have been generated against a diverse array of targets. Some of these have become indispensable reagents in biomedical research, while others were developed for novel therapeutic applications. The attractiveness of an- bodies in this regard is obvious—high target specificity, adaptability to a wide range of disease states, and the potential ability to direct the host’s immune s- tem for a therapeutic response. The initial excitement in finding Paul Ehrlich’s “magic bullet,” however, was met with widespread disappointment when it was demonstrated that murine antibodies frequently elicit the human anti-murine an- body (HAMA) response, thus rendering them ineffective and potentially unsafe in humans. Despite this setback, advances in recombinant DNA techniques over the last 15–20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to avoid HAMA. The ability to p- duce bulk quantities of recombinant proteins from bacterial fermentation also fueled the design of numerous creative antibody constructs. To date, the United States Food and Drug Administration has approved more than 10 recombinant antibodies for human use, and hundreds more are in the development pipeline. The recent explosion in genomic and proteomic information appears ready to deliver many more disease targets amenable to antibody-based therapy.
Decoding The Antibody Repertoire
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Author : Brandon DeKosky
language : en
Publisher: Springer
Release Date : 2017-05-29
Decoding The Antibody Repertoire written by Brandon DeKosky and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-29 with Medical categories.
This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases. Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells. The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
Neutralizing Antibodies And Their Role In Health And Disease
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Author : CHENGUANG. SHEN
language : en
Publisher:
Release Date : 2024
Neutralizing Antibodies And Their Role In Health And Disease written by CHENGUANG. SHEN and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024 with categories.
Design Production And Characterization Of Peptide Antibodies
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Author : Nicole Hartwig Trier
language : en
Publisher: Mdpi AG
Release Date : 2023-07-05
Design Production And Characterization Of Peptide Antibodies written by Nicole Hartwig Trier and has been published by Mdpi AG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-07-05 with Science categories.
Antibodies are key reagents in diagnostics and experimental biology, capable of detecting numerous antigenic targets. Proteins are often in focus and are usually effective targets for antibody production. Occasionally, however, the native protein is known but not available, or a very specific target is required. In these cases, synthetic peptides, copying essential sequences from the target, are good alternatives for antibody production. Peptide antibodies have been used in diagnostics and experimental biology with great success, especially because they can be produced to multiple targets, for example, native and denatured targets. Traditional peptide antibody proteins encompass animal-based immunization with a synthetic peptide, usually conjugated to a carrier protein to enhance immune presentation, as small peptides tend not to be immunogenic by themselves. There are several strategies for the conjugation of peptides to carriers applied for immunization. This is the most used approach for peptide antibody production. In addition to traditional peptide antibody production, peptide antibodies can be produced using libraries or sequencing. This Special Issue, "Design, Production and Characterization of Peptide Antibodies", aims to describe the current state-of-the-art techniques and characterization/applications within the field as well as new and emerging uses of peptide antibodies.