Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells
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Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells
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Author : Michael Edward Urbanowski
language : en
Publisher:
Release Date : 2012
Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells written by Michael Edward Urbanowski and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Hydrogen-ion concentration categories.
The regulation of pH is a critical homeostatic function of plant cells. In addition to acting as the primary cationic species responsible for energizing the plasma membrane, protons likely act as an important regulator and messenger. Despite this importance, few studies have thoroughly described cytosolic pH patterns as the plant cell progresses through the cell cycle. To investigate pH in plant cells, I chose Nicotiana tabacum (tobacco) Bright Yellow-2 (BY-2) cells as a model system. My research has two aims. First, I will measure and report the interphase cytosolic pH of BY-2 cells. Next, I will assay the cytosolic pH as BY-2 cells progress through mitosis and cytokinesis. I hypothesize that pH patterns are be temporally or spatially associated with structures such as the mitotic spindle or the phragmoplast. To investigate cytosolic pH in BY-2 cells, I will develop a cytosolic pH reporter based on a pH sensitive ratiometric fluorescent dye. This dye will be able to resolve both temporal and spatial changes in pH throughout the cytosol while imposing a minimal amount of stress on BY-2 cells. I found that pH-GFP, a variant of eGFP, had qualities of a robust pH reporter. To introduce the dye, explored biolistic bombardment, Agrobacterium mediated transient transformation, and polyethylene glycol mediated transformation as methods for introducing the pH-GFP gene into BY-2 cells. I observed very few transformation events using these methods and my observations did not support these approaches as suitable for introducing pH-GFP into BY-2 cells.
Tobacco By 2 Cells From Cellular Dynamics To Omics
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Author : Toshiyuki Nagata
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-09-22
Tobacco By 2 Cells From Cellular Dynamics To Omics written by Toshiyuki Nagata and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-09-22 with Technology & Engineering categories.
The tobacco BY-2 cell system is a unique model cell line for the study of dynamic features of plant cells. As extension of Volume 53, Tobacco BY-2 Cells, which presented basic aspects of the cell system, this present volume provides a wealth of new approaches. This latest volume in the series is an invaluable source of information for scientists in basic and applied plant biology.
Tobacco By 2 Cells
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Author : Toshiyuki Nagata
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Tobacco By 2 Cells written by Toshiyuki Nagata and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Technology & Engineering categories.
The first compilation of a wealth of knowledge on tobacco BY-2 cells, often cited as the HeLa cell line of higher plants. Basic issues of cell cycle progression, cytokinesis, cell organization and factors that are involved in these processes are covered in detail. Since the tobacco cell line is used as a tool for research in molecular and cellular biology, several chapters on such studies are also included. Further, changes of primary and secondary metabolites during culture and factors that affect these processes are treated. Last but not least, the so far unpublished historical background of the BY-2 cell line is described. This volume is a must for any scientist working in the field of plant biology.
The Plant Cell Cycle
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Author : Dirk Inzé
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-06-27
The Plant Cell Cycle written by Dirk Inzé and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-27 with Science categories.
In recent years, the study of the plant cell cycle has become of major interest, not only to scientists working on cell division sensu strictu , but also to scientists dealing with plant hormones, development and environmental effects on growth. The book The Plant Cell Cycle is a very timely contribution to this exploding field. Outstanding contributors reviewed, not only knowledge on the most important classes of cell cycle regulators, but also summarized the various processes in which cell cycle control plays a pivotal role. The central role of the cell cycle makes this book an absolute must for plant molecular biologists.
Cell Penetrating Peptides
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Author : Makoto Oba
language : en
Publisher: John Wiley & Sons
Release Date : 2023-02-13
Cell Penetrating Peptides written by Makoto Oba and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-13 with Science categories.
The definitive reference on the rational design of cell-penetrating peptides enables readers to develop tailor-made peptides for their specific needs. In recent years, cell-penetrating peptides (CPPs) have become valuable tools for the cellular delivery of proteins, nucleic acids, and drugs. These small peptide sequences can be artificially designed and synthesized with custom-made characteristics to mediate the efficient and non-toxic transport of biomolecules, drugs, or nanoparticles into the cell. Cell-Penetrating Peptides: Design, Development, and Applications provides an up-to-date account of the development and use of CPPs for delivering membrane-impermeable bioactive molecules into cells. Bringing together contributions from leading researchers from around the world, this comprehensive volume describes the characteristics and mechanisms of CPPs as well as their application in both medicine, biotechnology and agriculture. Covers rational design and development of cell-penetrating peptides for use in cellular delivery of small molecule drugs, proteins, nucleic acids, and nanoparticles Presents the chemical and biological characteristics of CPP action in vitro and in vivo Describes the structure and design principles of both synthetic and naturally occurring CPPs Discusses key medical applications of CPPs such as oral delivery, intranasal delivery, and clinical trials Cell-Penetrating Peptides: Design, Development, and Applications is an essential resource for biochemists, medicinal chemists, molecular biologists, biotechnologists, and researchers studying CPPs in both academia and industry.
Reactive Oxygen Species In Plant Signaling
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Author : Luis A. del Río
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-06-22
Reactive Oxygen Species In Plant Signaling written by Luis A. del Río and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-06-22 with Science categories.
Oxygen (O ) appeared in significant amounts in the Earth’s atmosphere over 2. 2 2 billion years ago, largely due to the evolution of photosynthesis by cyanobacteria (Halliwell 2006). The O molecule is a free radical, as it has two impaired electrons 2 that have the same spin quantum number. This spin restriction makes O prefer to 2 accept its electrons one at a time, leading to the generation of the so-called reactive oxygen species (ROS). The chemical nature of these species dictates that they can create damage in cells. This has contributed to the creation of the “oxidative stress” concept; in this view, ROS are unavoidable toxic products of O metabolism and 2 aerobic organisms have evolved antioxidant defences to protect against this tox- ity (Halliwell 1981; Fridovich 1998). Indeed, even in present-day plants, which are full of antioxidants, much of the protein synthetic activity of chloroplasts is used to replace oxidatively damaged D1 and other proteins (Halliwell 2006). Yet, the use of the “oxidative stress” term implies that ROS exert their effects through indiscriminate widespread inactivation of cellular functions. In this context, ROS must not be able to react with lipids, proteins or nucleic acids in order to avoid any damage to vital cellular components. However, genetic evidence has suggested that, in planta, purely physicoche- cal damage may be more limited than previously thought (Foyer and Noctor 2005).
Regulation Of Pollen Tube Growth Volume Ii
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Author : Stefano Del Duca
language : en
Publisher: Frontiers Media SA
Release Date : 2023-07-26
Regulation Of Pollen Tube Growth Volume Ii written by Stefano Del Duca and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-07-26 with Science categories.
Cumulated Index Medicus
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Author :
language : en
Publisher:
Release Date : 1999
Cumulated Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medicine categories.
Evolution Of Membrane Signaling And Trafficking In Plants
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Author : Markus Geisler
language : en
Publisher: Frontiers E-books
Release Date : 2013-06-12
Evolution Of Membrane Signaling And Trafficking In Plants written by Markus Geisler and has been published by Frontiers E-books this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-12 with categories.
Membrane proteins are essential determinants of many biological processes in plants. They function in metabolic processes, signal transduction, transport of small molecules and polymers across endo- and plasma membranes, and intercompartmental trafficking of proteins, lipids, and cell wall components. During these integrative processes, dynamic interactions of membrane proteins with other membrane or soluble components are thought to provide a high degree of flexibility that usually characterizes higher plants. This concept is supported by the recent release of a first, partial Arabidopsis interactome by the Arabidopsis Interactome Mapping Consortium (http://www.sciencemag.org/content/333/6042/601.full.htm). The Arabidopsis interactome reveals a strong enrichment of a few network communities, including those for transmembrane transport and vesicle trafficking. Strikingly, the large transmembrane transport community shares a high amount of proteins with the vesicle trafficking community suggesting a strong physical and functional overlap and interaction.
Root Biology
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Author : Bhoopander Giri
language : en
Publisher: Springer
Release Date : 2018-05-01
Root Biology written by Bhoopander Giri and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-01 with Technology & Engineering categories.
The book ‘Root Biology’ written by experts in the field, covers latest research on cellular, genetic, physiological and ecological developmental facets of root growth as well as the interaction of root with an array of microbes whether for the establishment of symbiosis, increasing plant growth or protecting plant from pathogens/attackers. Plant roots provide an excellent model to study physiological, developmental and metabolic processes at a system level. Root system architecture - an excellent creation of nature, is closely interconnected with the availability of soil nutrients. Several strategies including biotechnological interventions are gaining interest and importance for sustainable food production and enhanced resource acquisition. Such strategies have largely focused on root traits for efficient utilization of soil resources. The biotechnological application of root biology is expected to promote the production of food while maintaining ecologically and economically sustainable production systems. With a fortune of information on technical and experimental aspects useful in the laboratory, this extensive book is a valuable resource for researchers, academician and students in the broad field of microbiology, plant and fungal biology.