Tobacco By 2 Cells
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Tobacco By 2 Cells
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Author : Toshiyuki Nagata
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Tobacco By 2 Cells written by Toshiyuki Nagata and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Technology & Engineering categories.
The first compilation of a wealth of knowledge on tobacco BY-2 cells, often cited as the HeLa cell line of higher plants. Basic issues of cell cycle progression, cytokinesis, cell organization and factors that are involved in these processes are covered in detail. Since the tobacco cell line is used as a tool for research in molecular and cellular biology, several chapters on such studies are also included. Further, changes of primary and secondary metabolites during culture and factors that affect these processes are treated. Last but not least, the so far unpublished historical background of the BY-2 cell line is described. This volume is a must for any scientist working in the field of plant biology.
Tobacco By 2 Cells From Cellular Dynamics To Omics
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Author : Toshiyuki Nagata
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-09-22
Tobacco By 2 Cells From Cellular Dynamics To Omics written by Toshiyuki Nagata and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-09-22 with Technology & Engineering categories.
The tobacco BY-2 cell system is a unique model cell line for the study of dynamic features of plant cells. As extension of Volume 53, Tobacco BY-2 Cells, which presented basic aspects of the cell system, this present volume provides a wealth of new approaches. This latest volume in the series is an invaluable source of information for scientists in basic and applied plant biology.
Long Term Maintenance Of Tobacco By 2 Cells In Culture
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Author : Mette-Margrethe Redman
language : en
Publisher:
Release Date : 1996
Long Term Maintenance Of Tobacco By 2 Cells In Culture written by Mette-Margrethe Redman and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Astronautics categories.
Study Of Prevacuolar Compartments In Tobacco By 2 Cells
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Author : Siu Chung Cheung
language : en
Publisher:
Release Date : 2006
Study Of Prevacuolar Compartments In Tobacco By 2 Cells written by Siu Chung Cheung and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Plant vacuoles categories.
Cell Cycle Modulation Of Ck2 Activity In Tobacco By 2 Cells
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Author :
language : en
Publisher:
Release Date : 1999
Cell Cycle Modulation Of Ck2 Activity In Tobacco By 2 Cells written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with categories.
Improving Tobacco Nicotiana Tabacum Suspension Cells As A Molecular Farming Platform
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Author : Manoj Kumar Mandal
language : en
Publisher: Fraunhofer Verlag
Release Date : 2011
Improving Tobacco Nicotiana Tabacum Suspension Cells As A Molecular Farming Platform written by Manoj Kumar Mandal and has been published by Fraunhofer Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
The first endogenous matrix-metalloproteinase NtMMP1 from tobacco BY-2 cells was characterized in detail. Transgenic BY-2 cell lines expressing antisense-RNA against different endogenous proteases (aspartic, cysteine, matrix-metallo and serine) separately (single-antisense) or simultaneously (multi-antisense) was generated. The inhibition of endogenous protease(s) in those lines was verified and the stability of recombinant protein(s) was tested.
Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells
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Author : Michael Edward Urbanowski
language : en
Publisher:
Release Date : 2012
Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells written by Michael Edward Urbanowski and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Hydrogen-ion concentration categories.
The regulation of pH is a critical homeostatic function of plant cells. In addition to acting as the primary cationic species responsible for energizing the plasma membrane, protons likely act as an important regulator and messenger. Despite this importance, few studies have thoroughly described cytosolic pH patterns as the plant cell progresses through the cell cycle. To investigate pH in plant cells, I chose Nicotiana tabacum (tobacco) Bright Yellow-2 (BY-2) cells as a model system. My research has two aims. First, I will measure and report the interphase cytosolic pH of BY-2 cells. Next, I will assay the cytosolic pH as BY-2 cells progress through mitosis and cytokinesis. I hypothesize that pH patterns are be temporally or spatially associated with structures such as the mitotic spindle or the phragmoplast. To investigate cytosolic pH in BY-2 cells, I will develop a cytosolic pH reporter based on a pH sensitive ratiometric fluorescent dye. This dye will be able to resolve both temporal and spatial changes in pH throughout the cytosol while imposing a minimal amount of stress on BY-2 cells. I found that pH-GFP, a variant of eGFP, had qualities of a robust pH reporter. To introduce the dye, explored biolistic bombardment, Agrobacterium mediated transient transformation, and polyethylene glycol mediated transformation as methods for introducing the pH-GFP gene into BY-2 cells. I observed very few transformation events using these methods and my observations did not support these approaches as suitable for introducing pH-GFP into BY-2 cells.
Sites Of Microtubule Reorganization In Tobacco By 2 Cells During Cell Cycle Progression
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Author :
language : en
Publisher:
Release Date : 1997
Sites Of Microtubule Reorganization In Tobacco By 2 Cells During Cell Cycle Progression written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Plant Cell Wall And The Cytoskeleton Involvement In The Elongation Of Tobacco By 2 Cells And Arabidopsis Roots
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Author :
language : en
Publisher:
Release Date : 2014
Plant Cell Wall And The Cytoskeleton Involvement In The Elongation Of Tobacco By 2 Cells And Arabidopsis Roots written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Genetic Engineering Of The Primary Secondary Metabolic Interface In Tobacco By 2 Cells
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Author : Andrew M. Hall-Ponselè
language : en
Publisher:
Release Date : 2014
Genetic Engineering Of The Primary Secondary Metabolic Interface In Tobacco By 2 Cells written by Andrew M. Hall-Ponselè and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.