Diagnosis Of Human Viruses By Polymerase Chain Reaction Technology
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Diagnosis Of Human Viruses By Polymerase Chain Reaction Technology
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Author : Yechiel Becker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Diagnosis Of Human Viruses By Polymerase Chain Reaction Technology written by Yechiel Becker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
The basis for the effective treatment and cure of a patient is the rapid diagnosis of the disease and its causative agent, which is based on the analysis of the clinical symptoms coupled with laboratory tests. Although rapid advance ments have been made in the laboratory diagnosis of virus diseases, the neces sary isolation of the causative virus from the clinical specimens is a relatively long procedure. Viruses which integrate into the cellular DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are difficult to identify by molecular techniques, while viruses which exist in the clinical material in low concentrations are even more formidable to identify. Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (1985) led to a revolution in virus diagnosis. The peR technique was rapidly applied to the diagnosis of viruses in clinical material. Volume 1 of Frontiers of Virology provides new information on the advan tages of the use of the peR for the diagnosis of many human disease-causing viruses, as well as on some problems with its use.
The Polymerase Chain Reaction Pcr For Human Viral Diagnosis
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Author : Jonathan P. Clewley
language : en
Publisher: CRC Press
Release Date : 1994-10-27
The Polymerase Chain Reaction Pcr For Human Viral Diagnosis written by Jonathan P. Clewley and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-10-27 with Science categories.
The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.
Molecular Methods For Virus Detection
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Author : Danny L. Wiedbrauk
language : en
Publisher: Elsevier
Release Date : 1995-02-08
Molecular Methods For Virus Detection written by Danny L. Wiedbrauk and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-02-08 with Science categories.
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables
Pcr Protocols For Diagnosis Of Human And Animal Virus Diseases
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Author : Yechiel Becker
language : en
Publisher: Springer
Release Date : 1995-09-26
Pcr Protocols For Diagnosis Of Human And Animal Virus Diseases written by Yechiel Becker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-09-26 with Medical categories.
Today the diagnosis of human and animal pathogenic viruses would be unthinkable without the polymerase chain reaction (PCR). The short-styled protocols in this volume elucidate this important technique and its application in the laboratory, including a discussion of recent advances in PCR technology. The enclosed diskette enables the user to work with interesting sequences on his own computer.
Lennette S Laboratory Diagnosis Of Viral Infections
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Author : Keith R Jerome
language : en
Publisher: CRC Press
Release Date : 2016-04-19
Lennette S Laboratory Diagnosis Of Viral Infections written by Keith R Jerome and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Medical categories.
Written from the perspective of the diagnostician, this bestselling book is the definitive text on the laboratory diagnosis of human viral diseases. It contains a wealth of illustrations, tables, and algorithms to enhance your understanding of this ever-evolving field. The book is a ready reference for virologists, microbiologists, epidemiologists, laboratorians, and infections disease specialists, and students.
Diagnostic Virology Protocols
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Author : John R. Stephenson
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-08-28
Diagnostic Virology Protocols written by John R. Stephenson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-08-28 with Medical categories.
A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.
Sustainable Disease Management In A European Context
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Author : David B. Collinge
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-01-23
Sustainable Disease Management In A European Context written by David B. Collinge and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-23 with Science categories.
The main theme of the book is sustainable disease management in a European context. Some of the questions addressed are: How does society benefit from plant pathology research? How can new molecular approaches solve relevant problems in disease management? What other fields can we exploit in plant pathology research? What challenges are associated with free trade across the new borders? How can we contribute to solving problems of developing countries? How does plant pathology contribute to food quality and safety? How does globalization/internationalization affect teaching and extension in plant pathology?
Nucleic Acid Amplification Technologies
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Author : Helen H. Lee
language : en
Publisher: Springer Science & Business Media
Release Date : 1997-07-15
Nucleic Acid Amplification Technologies written by Helen H. Lee and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-07-15 with Medical categories.
Providing current information and guidance on the uses of various nucleic acid amplification technologies for clinical laboratory diagnosis, this book goes beyond the Polymerase Chain Reaction to explore a broader range of important alternative DNA/RNA amplification methods including the Ligase Chain Reaction, Q[beta] Replicase Assays and TMA. There are many examples of specific applications of these technologies, discussions of yet unresolved issues and demonstrations of the relevance of these technologies to medical research and disease diagnostics. Individual chapters cover uses of these methods in clinical situations such as detection of food pathogens, viral infections, STDs, Mycobacteria drug resistance mutations, and heritable diseases. Automation, diagnostic test evaluation, and the synthesis of artificial DNA are also discussed. This book is designed for all biomedical scientists interested in the application of molecular biology to clinical diagnosis.
Viral Diagnostics
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Author : Robert S. Marks
language : en
Publisher: CRC Press
Release Date : 2014-12-15
Viral Diagnostics written by Robert S. Marks and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-12-15 with Medical categories.
This book illustrates a variety of challenges that bug hunters try to solve. It is an outstanding collection of the insights and expertise of an interdisciplinary group of researchers from all walks of life: virologists, physicians, immunologists, electrochemists, physicists, computer scientists, biotechnologists, epidemiologists, and molecular bio
Methods In Dna Amplification
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Author : Ulrich Finckh
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods In Dna Amplification written by Ulrich Finckh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rapidly evolving field. We demonstrate, for example how to use fixed tissue materials and how to quantify PCR products as well as how to prepare nucleic acids in a safe, convenient and proper way, or even how to sequence directly PCR products for the analysis of the DNA structure.