Digital Microfluidic Sample Preparation For Biological Mass Spectrometry
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Digital Microfluidic Sample Preparation For Biological Mass Spectrometry
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Author : Adam A. Stokes
language : en
Publisher:
Release Date : 2011
Digital Microfluidic Sample Preparation For Biological Mass Spectrometry written by Adam A. Stokes and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
The use of mass spectrometry in the biosciences has undergone huge growth in re- cent years due to sustained effort in the development of new ionisation techniques, more powerful mass analysers and better bioinformatic tools. These developments mean that it is now possible to introduce complex crude biological-mixtures into a mass spectrometric platform and to obtain detailed information about the sample. The front-end sample handling techniques used for sample preparation have, for the most part, not changed despite the recent advances in hyphenation of liquid- chromatography and mass spectrometry required to tackle the issue of increased sample complexity. In this thesis the possibility of using Digital Microfluidics (DMF) for front-end sample preparation prior to mass-spectrometric analysis of protein samples has been investigated. DMF is a micro-electromechanical system (MEMS) technology used for manipulation of sub-microlitre droplets. The movement of discrete droplets of liquid is exploited using the Coulombic forces arising due to free charge polarisation. Droplets can be split, joined, dispensed and moved over a sub-surface electrode array. In this thesis a range of DMF devices have been designed, manufactured and coupled with mass spectrometric platforms for protein analysis. A variety of techniques for mass spectrometry- based analysis of biological samples from the fluidic chips have been investigated. A robotic system has been developed to automate sample introduction, manipulation and removal. Finally the application of on-chip sample purification and enzymatic digestion have been demonstrated, providing proof of concept for digital microfluidic sample preparation in mass spectrometry-based proteomics.
Sample Preparation In Biological Mass Spectrometry
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Author : Alexander R. Ivanov
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-06-15
Sample Preparation In Biological Mass Spectrometry written by Alexander R. Ivanov and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-15 with Science categories.
The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.
Encyclopedia Of Microfluidics And Nanofluidics
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Author : Dongqing Li
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-08-06
Encyclopedia Of Microfluidics And Nanofluidics written by Dongqing Li and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-08-06 with Technology & Engineering categories.
Covering all aspects of transport phenomena on the nano- and micro-scale, this encyclopedia features over 750 entries in three alphabetically-arranged volumes including the most up-to-date research, insights, and applied techniques across all areas. Coverage includes electrical double-layers, optofluidics, DNC lab-on-a-chip, nanosensors, and more.
Molecular Diagnostics
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Author : George P. Patrinos
language : en
Publisher: Academic Press
Release Date : 2016-10-27
Molecular Diagnostics written by George P. Patrinos and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-27 with Medical categories.
Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. - Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories - Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression - Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field
Droplet And Digital Microfluidics
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Author : Sanket Goel
language : en
Publisher: Elsevier
Release Date : 2024-03-11
Droplet And Digital Microfluidics written by Sanket Goel and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-03-11 with Technology & Engineering categories.
Droplet and Digital Microfluidics: Ideation to Implementation is a detailed introduction to the dynamics of droplet and digital microfluidics, also featuring coverage of new methods and applications. The explosion of applications of microelectromechanical systems (MEMS) in recent years has driven demand for expertise and innovation in fluid flow in the microchannels they contain. In this book, detailed descriptions of methods for biological and chemical applications of microfluidics are provided, along with supporting foundational knowledge. In addition, the principles of droplet and digital microfluidics are explained, along with their different applications and governing physics.New additions to the technological knowledgebase that enable advances in droplet and digital microfluidics include machine learning and exciting future avenues for research. - Provides step-by-step fabrication, testing, and characterization instructions in each chapter to support implementation - Includes explanations of applications and methods in biological and chemical settings - Describes the path to automation of digital and droplet microfluidic platforms
Handbook Of Capillary And Microchip Electrophoresis And Associated Microtechniques
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Author : James P. Landers
language : en
Publisher: CRC Press
Release Date : 2007-12-18
Handbook Of Capillary And Microchip Electrophoresis And Associated Microtechniques written by James P. Landers and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-18 with Medical categories.
Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.
Design And Testing Of Digital Microfluidic Biochips
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Author : Yang Zhao
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-07-25
Design And Testing Of Digital Microfluidic Biochips written by Yang Zhao and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-25 with Technology & Engineering categories.
This book provides a comprehensive methodology for automated design, test and diagnosis, and use of robust, low-cost, and manufacturable digital microfluidic systems. It focuses on the development of a comprehensive CAD optimization framework for digital microfluidic biochips that unifies different design problems. With the increase in system complexity and integration levels, biochip designers can utilize the design methods described in this book to evaluate different design alternatives, and carry out design-space exploration to obtain the best design point.
Nanotechnology For Microfluidics
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Author : Xingyu Jiang
language : en
Publisher: John Wiley & Sons
Release Date : 2020-09-08
Nanotechnology For Microfluidics written by Xingyu Jiang and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-08 with Technology & Engineering categories.
The book focuses on microfluidics with applications in nanotechnology. The first part summarizes the recent advances and achievements in the field of microfluidic technology, with emphasize on the the influence of nanotechnology. The second part introduces various applications of microfluidics in nanotechnology, such as drug delivery, tissue engineering and biomedical diagnosis.
Microfluidics And Lab On A Chip
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Author : Andreas Manz
language : en
Publisher: Royal Society of Chemistry
Release Date : 2020-09-24
Microfluidics And Lab On A Chip written by Andreas Manz and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-24 with Science categories.
Responding to the need for an affordable, easy-to-read textbook that introduces microfluidics to undergraduate and postgraduate students, this concise book will provide a broad overview of the important theoretical and practical aspects of microfluidics and lab-on-a-chip, as well as its applications.
Mass Spectrometry Based Single Cell Proteomics
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Author : Akos Vegvari
language : en
Publisher: Springer Nature
Release Date : 2024-06-21
Mass Spectrometry Based Single Cell Proteomics written by Akos Vegvari and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-21 with Science categories.
This volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book focus on technologies, methods, and biological applications and together cover the entire single cell proteomics analysis workflow, from sample collection and preparation through liquid chromatographic separation and mass spectrometric data acquisition to data analysis and interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Mass Spectrometry Based Single Cell Proteomics: Methods and Protocols is a valuable resource for all researchers interested in using these technologies and methods in their laboratories to continue making biological discoveries in this important field.