Dna Manipulation And Analysis

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Dna Manipulation And Analysis

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Author : Garry Scarlett
language : en
Publisher: Springer Nature
Release Date : 2023-02-28

Dna Manipulation And Analysis written by Garry Scarlett and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-28 with Science categories.

This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.

Micro And Nanomanipulation Tools

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Author : Yu Sun
language : en
Publisher: John Wiley & Sons
Release Date : 2015-08-24

Micro And Nanomanipulation Tools written by Yu Sun and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-08-24 with Technology & Engineering categories.

Combining robotics with nanotechnology, this ready reference summarizes the fundamentals and emerging applications in this fascinating research field. This is the first book to introduce tools specifically designed and made for manipulating micro- and nanometer-sized objects, and presents such examples as semiconductor packaging and clinical diagnostics as well as surgery. The first part discusses various topics of on-chip and device-based micro- and nanomanipulation, including the use of acoustic, magnetic, optical or dielectrophoretic fields, while surface-driven and high-speed microfluidic manipulation for biophysical applications are also covered. In the second part of the book, the main focus is on microrobotic tools. Alongside magnetic micromanipulators, bacteria and untethered, chapters also discuss silicon nano- and integrated optical tweezers. The book closes with a number of chapters on nanomanipulation using AFM and nanocoils under optical and electron microscopes. Exciting images from the tiniest robotic systems at the nano-level are used to illustrate the examples throughout the work. A must-have book for readers with a background ranging from engineering to nanotechnology.

Recombinant Dna Principles And Methodologies

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Author : James Greene
language : en
Publisher: CRC Press
Release Date : 2021-12-16

Recombinant Dna Principles And Methodologies written by James Greene and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-16 with Science categories.

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Unraveling Genetic Mysteries Longevity Genetic Engineering And Philosophy Of Mind

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Author : Edenilson Brandl
language : en
Publisher: Edenilson Brandl
Release Date :

Unraveling Genetic Mysteries Longevity Genetic Engineering And Philosophy Of Mind written by Edenilson Brandl and has been published by Edenilson Brandl this book supported file pdf, txt, epub, kindle and other format this book has been release on with Health & Fitness categories.

Genetics is a fascinating and complex field, which reveals the secrets of the genetic code present in every living being. Through the investigation of genes and their interaction with the environment, we are able to better understand the biological processes that shape our existence. In this book, we'll explore the wonders and challenges of genetics, as well as its applications in areas such as medicine, criminal investigation, and genetic engineering. The first part of this book will take us through the intricate world of the genetics of aging and longevity. We will investigate the genetic mechanisms that determine the rate of aging of organisms and explore the possibilities of prolonging a healthy life. We'll discover how the latest research is unlocking the mysteries of aging and offering new insights into human longevity. Then we will enter the field of genetic engineering and genetically modified organisms (GMOs). We will explore the innovative techniques that allow the manipulation of DNA and the creation of organisms with specific characteristics. We'll see how this technology is revolutionizing agriculture, medicine, and industry, while raising important ethical and environmental questions. Forensic genetics will also gain prominence on our journey. We will investigate how DNA analysis has become a powerful tool in solving crimes, unraveling mysteries and assisting in the pursuit of justice. We'll discover how technological advances are revolutionizing criminal investigation and providing a deeper understanding of the genetic bonds between individuals. Entering the field of health, we will explore the genetics of neurodegenerative, cardiovascular, autoimmune and psychiatric disorders. We will understand how genes play a key role in the development of these diseases and how this understanding can open the way to more effective treatments and prevention strategies. In addition, we will investigate pharmacogenomics and precision medicine, examining how genetic individuality influences individuals' response to medications, enabling personalized and more effective treatments. We'll also cover the genetics of metabolism and obesity, sleep disorders, and the genetic implications for these conditions. The second part of this book will take us on a philosophical journey into the world of the mind. We'll explore debates about the nature of consciousness, the mind-body problem, reductionism, and dualism. We will investigate the relationship between the mind and the brain, the question of personal identity over time, and the philosophical implications of the mind in relation to artificial intelligence, free will, neuroscience, qualia, and even animal consciousness.

Synthetic Biology Genetics And Advanced Topics

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Author : Edenilson Brandl
language : en
Publisher: Edenilson Brandl
Release Date :

Synthetic Biology Genetics And Advanced Topics written by Edenilson Brandl and has been published by Edenilson Brandl this book supported file pdf, txt, epub, kindle and other format this book has been release on with Health & Fitness categories.

Synthetic Biology is a scientific discipline that has the potential to revolutionize the way we understand and manipulate life. Combining knowledge from biology, genetics, engineering and computing, Synthetic Biology opens up new possibilities for creating personalized biological systems, with applications ranging from regenerative medicine to the production of biofuels. In this book, we will explore the principles, techniques, and applications of synthetic biology, with the aim of providing a comprehensive and up-to-date overview of this ever-evolving field. Throughout the chapters, we will delve into the molecular foundations of Synthetic Biology, exploring the biological components and processes involved in building synthetic biological systems. We will present several applications of Synthetic Biology in different sectors, highlighting its role in the pharmaceutical industry, in the production of biofuels, in the development of personalized vaccines and in regenerative medicine. We will see how the manipulation of synthetic stem cells can revolutionize the area of tissue regeneration and how metabolic engineering allows us to design microorganisms capable of producing valuable compounds. Bioinformatics plays a crucial role in Synthetic Biology, and we will dedicate a chapter to exploring how the analysis of genomic data and the use of bioinformatics algorithms contribute to the advancement of this discipline. In addition, we will cover topics such as genome editing using CRISPR technology, synthetic protein engineering, and directed evolution in the laboratory. With the advancement of technology, it has become possible to manufacture living tissue in the laboratory, and we will discuss biofabrication and 3D printing of living tissue, as well as the use of microfluidics in the manipulation and analysis of synthetic biological systems on a microscopic scale. We will also address ethical and biosafety issues related to Synthetic Biology, recognizing the importance of a responsible approach in all its applications. This book is intended for students, researchers, and practitioners interested in exploring the frontiers of synthetic biology. We hope it will be an inspiring source of knowledge, awakening the curiosity and innovative spirit of all readers. We invite you to embark on this exciting journey through Synthetic Biology, in search of a deeper understanding of life and its transformative possibilities.

Student Solutions Manual And Supplemental Problems To Accompany Genetics Analysis Of Genes And Genomes

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Author : Daniel Hartl
language : en
Publisher: Jones & Bartlett Publishers
Release Date : 2011-10-13

Student Solutions Manual And Supplemental Problems To Accompany Genetics Analysis Of Genes And Genomes written by Daniel Hartl and has been published by Jones & Bartlett Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-13 with Science categories.

This must-have student resource contains complete solutions to all end-of-chapter problems in Genetics: Analysis of Genes and Genomes, Eighth Edition, by Daniel L. Hartl and Maryellen Ruvolo, as well as a wealth of supplemental problems and exercises with full solutions, a complete chapter summary, and keyword section. The supplemental problems provided in this manual are designed as learning opportunities rather than exercises to be completed by rote. They are organized into chapters that parallel those of the main text, and all problems can be solved through application of the concepts and principles explained in Genetics, Eighth Edition.

Cloning

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Author : Stephen D. Fairbanks
language : en
Publisher: Nova Publishers
Release Date : 2004

Cloning written by Stephen D. Fairbanks and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.

The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.

Medical Biomethods Handbook

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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-09

Medical Biomethods Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-09 with Science categories.

John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.

Mathematical Support For Molecular Biology

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Author : Martin Farach-Colton
language : en
Publisher: American Mathematical Soc.
Release Date : 1999

Mathematical Support For Molecular Biology written by Martin Farach-Colton and has been published by American Mathematical Soc. this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Mathematics categories.

Contains papers related to the 1994-98 DIMACS "Special Year" in Mathematical Support for Biology, which involved a series of tutorials, workshops, and lectures. Papers reflect diverse work at the interface between the mathematical sciences and molecular biology. Topics include new uses for uniform lifted alignments, sequence alignment and phylogeny construction, formal language theory and biological macromolecules, parallel strategies for DNA manipulation analysis, and visualizing measures of genetic distance. No index. Annotation copyrighted by Book News, Inc., Portland, OR

Thrive In Biochemistry And Molecular Biology

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Author : Lynne Cox
language : en
Publisher: Oxford University Press
Release Date : 2012-05-31

Thrive In Biochemistry And Molecular Biology written by Lynne Cox and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-31 with Education categories.

The Thrive in Bioscience revision guides are written to help undergraduate students achieve exam success in all core areas of bioscience. They communicate all the key concepts in a succinct, easy-to-digest way, using features and tools - both in the book and in digital form - to make learning even more effective.