Dna Protein Interactions
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Dna Protein Interactions
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Author : Tom Moss
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Dna Protein Interactions written by Tom Moss and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
Dna Protein Interactions
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Author : A. Travers
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Dna Protein Interactions written by A. Travers and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Our understanding of the mechanisms regulating gene expression, which determine the patterns of growth and development in all living organisms, ultimately involves the elucidation of the detailed and dy namic interactions of proteins with nucleic acids -both DNA and RNA. Until recently the commonly presented view of the DNA double helix as visualized on the covers of many textbooks and journals - was as a monotonous static straight rod incapable in its own right of directing the processes necessary for the conservation and selective reading of genetic information. This view, although perhaps extreme, was reinforced by the necessary linearity of genetic maps. The reality is that the biological functions of both DNA and RNA are dependent on complex, and sometimes transient, three-dimensional nucleoprotein structures in which genetically distant elements are brought into close spatial proximity. It is in such structures that the enzymatic manipulation of DNA in the essential biological processes as DNA replication, transcription and recombination are effected - the complexes are the mediators of the 'DNA transactions' of Hatch Echols.
Dna Protein Interactions
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Author : A. Travers
language : en
Publisher: Springer Science & Business Media
Release Date : 1993-04-30
Dna Protein Interactions written by A. Travers and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-04-30 with Medical categories.
The binding of proteins to DNA and the manipulation of DNA by proteins are crucial aspects of the biological role of DNA in the living cell. This book provides a comprehensive and lucid discussion of the molecular interactions involved.
Biophysics Of Dna Protein Interactions
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Author : Mark C. Williams
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-10-05
Biophysics Of Dna Protein Interactions written by Mark C. Williams and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-05 with Science categories.
Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has developed very quickly, and a few books specifically treating single molecule methods are beginning to appear. However, the promise of single molecule biophysics is to contribute to the understanding of specific fields of biology using new methods. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods. This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than just single molecule biophysicists or even biophysicists. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA. For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on DNA. A few chapters will be devoted to experiments and theory that shed light on this important problem. We will also cover proteins that alter DNA properties to facilitate interactions important for transcription or replication. Another section of the book will cover the biophysical mechanism by which motor proteins interact with DNA. Finally, we will cover larger protein-DNA complexes, such as replication forks, recombination complexes, DNA repair interactions, and their chromatin context.
Dna Protein Interactions In Transcription
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Author : Jay D. Gralla
language : en
Publisher: Alan R. Liss
Release Date : 1989
Dna Protein Interactions In Transcription written by Jay D. Gralla and has been published by Alan R. Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Science categories.
Dna Protein Interactions
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Author : Tom Moss
language : en
Publisher: Humana
Release Date : 2010-11-19
Dna Protein Interactions written by Tom Moss and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-19 with Science categories.
Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. Addressing topics such as chromatin immunoprecipitation, topological studies, photocrosslinking, FRET and imaging techniques, the volume fully updates and expands upon the successful previous editions. Written in the convenient and informative Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, DNA-Protein Interactions: Principles and Protocols, Third Edition serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research.
Dna Protein Interactions
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Author : Andrew Arthur Travers
language : en
Publisher: Oxford University Press, USA
Release Date : 2000
Dna Protein Interactions written by Andrew Arthur Travers and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure. Knowledge of basic techniques is assumed, although advances in fundamental fields are covered.
Dna Protein Interactions
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Author : Marcos Simoes-Costa
language : en
Publisher: Springer Nature
Release Date : 2022-11-25
Dna Protein Interactions written by Marcos Simoes-Costa and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-25 with Science categories.
This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies.
The Biology Of Nonspecific Dna Protein Interactions
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Author : Arnold Revzin
language : en
Publisher: CRC Press
Release Date : 1990-08-27
The Biology Of Nonspecific Dna Protein Interactions written by Arnold Revzin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-08-27 with Medical categories.
This important publication addresses the interactions of proteins with nonspecific binding sites on DNA as they play critical roles in fundamental cellular processes such as transcription, DNA replication, and recombination. The book presents current reviews of the biochemistry of representative nonspecific DNA-protein systems, and of their physiological functions. It includes chapters on the techniques used to characterize the complexes, on their thermodynamic properties, and on the role of nonspecific binding as gene regulatory proteins search for specific target sites on the chromosome. Systems considered include the effects of nonspecific binding in regulation of the lactose operon of Escherichia coli, the T4 bacteriophage gene 32 protein, the E. coli single strand binding (SSB) protein and recA protein, eukaryotic SSB's and histone-DNA complexes. The book presents those proteins displaying multiple modes of DNA binding as participants in more than one cellular process. This monograph combines rigorous descriptions of new findings for these important systems with provocative interpretations of the biological significance of the results. It is of great value to researchers ranging from graduate students to senior scientists in the areas of biochemistry, microbiology and molecular/cell biology.
Analytics Of Protein Dna Interactions
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Author : Harald Seitz
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-01-16
Analytics Of Protein Dna Interactions written by Harald Seitz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-16 with Medical categories.
With contributions by numerous experts