Electron Microscopy Of Plant Pathogens
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Electron Microscopy Of Plant Pathogens
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Author : Kurt Mendgen
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Electron Microscopy Of Plant Pathogens written by Kurt Mendgen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Electron Microscopy Of Plant Pathogens
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Author : Kurt Mendgen
language : en
Publisher: Springer
Release Date : 1990-12-10
Electron Microscopy Of Plant Pathogens written by Kurt Mendgen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-12-10 with Science categories.
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Plant Pathogen Detection And Disease Diagnosis Second Edition
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Author : P. Narayanasamy
language : en
Publisher: CRC Press
Release Date : 2001-08-28
Plant Pathogen Detection And Disease Diagnosis Second Edition written by P. Narayanasamy and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-08-28 with Science categories.
This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.
Molecular Methods In Plant Pathology
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Author : U. S. Singh
language : en
Publisher: CRC Press
Release Date : 2017-12-14
Molecular Methods In Plant Pathology written by U. S. Singh and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-14 with Science categories.
Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
Laboratory Exercises In Plant Pathology
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Author : A. B. A. M. Baudoin
language : en
Publisher: Amer Phytopathological Society
Release Date : 1988
Laboratory Exercises In Plant Pathology written by A. B. A. M. Baudoin and has been published by Amer Phytopathological Society this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Science categories.
How to use this kit; Use and care of the light microscope; Recognition and terminology of disease symptoms and signs; Isolation of fungi and bacteria; Diagnosis of disease and proof of pathogenicity; Plant disease diagnosis in practice; Characteristics and structures of fungi; Root infection by phytophtora; Damping-off of seeds and seedlings; Soils fungistasis; Pathogen survival in soil; Biological control of soilborne plant pathogens by antagonistic fungi; Soilborne fungi; Population assessment of fusarium spp; Foliar infection by fungi; Dissemination of fungus spores and bacterial cells by wind and water; Fungicides for the control of foliar infection; Race identification of wheat leaf rust; Bacterial pathogens; Crown gall; Frost injury and ice nucleating bacteria; Viruses: mechanical transmission and host range; Insect transmission of plant viruses; Electron microscopy; Virus identification by serology; Study of plant-parasitic nematodes; Root-Knot nematodes: Symptoms, life stages, and control; Cyst nematodes; Foliar nematodes on begonia: Physiological spects of plant disease; Abiotic diseases; Disorders caused by higher plant.
Methods In Plant Electron Microscopy And Cytochemistry
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Author : William V. Dashek
language : en
Publisher: Springer Science & Business Media
Release Date : 2000-06-29
Methods In Plant Electron Microscopy And Cytochemistry written by William V. Dashek and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-06-29 with Science categories.
Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Electron Microscopy And Plant Ultrastructure
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Author : Anthony William Robards
language : en
Publisher:
Release Date : 1970
Electron Microscopy And Plant Ultrastructure written by Anthony William Robards and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1970 with Science categories.
Diagnosis Of Plant Virus Diseases
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Author : R. E. F. Matthews
language : en
Publisher: CRC Press
Release Date : 2019-07-23
Diagnosis Of Plant Virus Diseases written by R. E. F. Matthews and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-23 with Science categories.
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
Phytopathological Diagnostic Techniques
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Author : Iftikhar Ahmad
language : en
Publisher:
Release Date : 1992
Phytopathological Diagnostic Techniques written by Iftikhar Ahmad and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Plant diseases categories.
Electron Microscopy Of Plant Cells
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Author : C Hawes
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Electron Microscopy Of Plant Cells written by C Hawes and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.