Methods In Plant Electron Microscopy And Cytochemistry
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Methods In Plant Electron Microscopy And Cytochemistry
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Author : William V. Dashek
language : en
Publisher: Springer Science & Business Media
Release Date : 2000-06-29
Methods In Plant Electron Microscopy And Cytochemistry written by William V. Dashek and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-06-29 with Science categories.
Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Electron Microscopy And Cytochemistry Of Plant Cells
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Author : J. L. Hall
language : en
Publisher:
Release Date : 1978
Electron Microscopy And Cytochemistry Of Plant Cells written by J. L. Hall and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978 with Botanical microscopy categories.
Electron Microscopy Of Plant Cells
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Author : C Hawes
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Electron Microscopy Of Plant Cells written by C Hawes and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Methods In Cell Wall Cytochemistry
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Author : K V Krishnamurthy
language : en
Publisher: CRC Press
Release Date : 2020-07-24
Methods In Cell Wall Cytochemistry written by K V Krishnamurthy and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-24 with Science categories.
Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.) Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.
The Study Of Plant Structure
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Author : Terence P. O'Brien
language : en
Publisher:
Release Date : 1981
The Study Of Plant Structure written by Terence P. O'Brien and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with Science categories.
Techniques For Plant Electron Microscopy
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Author : Barrie Edward Juniper
language : en
Publisher: Blackwell Scientific Publications Limited
Release Date : 1970
Techniques For Plant Electron Microscopy written by Barrie Edward Juniper and has been published by Blackwell Scientific Publications Limited this book supported file pdf, txt, epub, kindle and other format this book has been release on 1970 with Science categories.
Fixation. Embedding. Microtomy. General stains. Cytochemical stains. Negative staining. Specimen support films. Replicas. Preparation for scanning electron microscopy. Special techniques. Autoradiography. The instrument and photography.
Electron Microscopy Of Plant Pathogens
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Author : Kurt Mendgen
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Electron Microscopy Of Plant Pathogens written by Kurt Mendgen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Plant Cell Biology
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Author : Chris Hawes
language : en
Publisher: OUP Oxford
Release Date : 2001-05-31
Plant Cell Biology written by Chris Hawes and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-05-31 with Science categories.
With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
A Manual Of Applied Techniques For Biological Electron Microscopy
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Author : Michael J. Dykstra
language : en
Publisher: Springer Science & Business Media
Release Date : 1993-08-31
A Manual Of Applied Techniques For Biological Electron Microscopy written by Michael J. Dykstra and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-08-31 with Medical categories.
This easy-to-follow manual describes tested procedures used to prepare biological samples for scanning and transmission electron microscopy, as well as methods for cytochemistry, immunocytochemistry, and scientific photography. The work is structured to clearly define testing objectives, necessary materials, procedural steps, and expected results; a list of references and trouble shooting techniques round out the text.
Hybridization Techniques For Electron Microscopy
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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 1993-06-09
Hybridization Techniques For Electron Microscopy written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-06-09 with Science categories.
Hybridization Techniques for Electron Microscopy examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. The book features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissue method using ultrathin frozen sections, pre-embedded method, and post-embedded method using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided. Particular aspects of the techniques are examined in detail, such as preparation of tissue, pretreatment, hybridization procedures, revelation (autoradiography and immunocytology) and checking procedures, in addition to the illustration, interpretation, and discussion of methods and results. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid, and detection of messenger RNA in animals and plants. Hybridization Techniques for Electron Microscopy is an excellent reference for cytologists, cell biologists, histochemists, cytochemists, molecular endocrinologists, and neuroendocrinologists.