Hybridization Techniques For Electron Microscopy

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Hybridization Techniques For Electron Microscopy

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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 1993-06-09

Hybridization Techniques For Electron Microscopy written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-06-09 with Science categories.

Hybridization Techniques for Electron Microscopy examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. The book features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissue method using ultrathin frozen sections, pre-embedded method, and post-embedded method using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided. Particular aspects of the techniques are examined in detail, such as preparation of tissue, pretreatment, hybridization procedures, revelation (autoradiography and immunocytology) and checking procedures, in addition to the illustration, interpretation, and discussion of methods and results. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid, and detection of messenger RNA in animals and plants. Hybridization Techniques for Electron Microscopy is an excellent reference for cytologists, cell biologists, histochemists, cytochemists, molecular endocrinologists, and neuroendocrinologists.

Electron Microscopy Methods And Protocols

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Author : M. A. Nasser Hajibagheri
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Electron Microscopy Methods And Protocols written by M. A. Nasser Hajibagheri and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

In Situ Hybridization In Electron Microscopy

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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 2001-01-30

In Situ Hybridization In Electron Microscopy written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-01-30 with Science categories.

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization

Electron Microscopy Of Plant Cells

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Author : C Hawes
language : en
Publisher: Academic Press
Release Date : 2012-12-02

Electron Microscopy Of Plant Cells written by C Hawes and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.

Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

Making And Using Antibodies

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Author : Gary C. Howard
language : en
Publisher: CRC Press
Release Date : 2013-07-29

Making And Using Antibodies written by Gary C. Howard and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-07-29 with Medical categories.

Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science-both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects

Principles And Techniques Of Electron Microscopy

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Author : M. A. Hayat
language : en
Publisher: John Wiley & Sons
Release Date : 1977

Principles And Techniques Of Electron Microscopy written by M. A. Hayat and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977 with Science categories.

Specimen damage in the electron microscopy; Freeze-drying for electron microscopy; Image reconstruction of electron micrographs by using equidensite integration analysis; G-banding of chromosomes; Autoradiographic localization of DNA in nonmetabolic conditions; Optical analysis and reconstruction of images; Mirror electron microscopy; Electron microscopy of banded mammalian chromosomes; Equidensitometry: some neurobiological applications.

Visualization Of Nucleic Acids

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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 1995-03-30

Visualization Of Nucleic Acids written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-03-30 with Science categories.

This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.

Morphology Methods

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Author : Ricardo V. Lloyd
language : en
Publisher: Springer Science & Business Media
Release Date : 2001-06-15

Morphology Methods written by Ricardo V. Lloyd and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-15 with Medical categories.

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Methods In Plant Cell Biology Part A

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Author :
language : en
Publisher: Elsevier
Release Date : 1995-10-10

Methods In Plant Cell Biology Part A written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-10-10 with Science categories.

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants

Handbook Of Molecular And Cellular Methods In Biology And Medicine

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Author : Leland J. Cseke
language : en
Publisher: CRC Press
Release Date : 2016-04-19

Handbook Of Molecular And Cellular Methods In Biology And Medicine written by Leland J. Cseke and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Medical categories.

Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology