Visualization Of Nucleic Acids
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Visualization Of Nucleic Acids
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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 1995-03-30
Visualization Of Nucleic Acids written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-03-30 with Science categories.
This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.
Genome Visualization By Classic Methods In Light Microscopy
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Author : Jean-Marie Exbrayat
language : en
Publisher: CRC Press
Release Date : 2000-11-27
Genome Visualization By Classic Methods In Light Microscopy written by Jean-Marie Exbrayat and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-11-27 with Science categories.
Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization. This book provides insight into several classic techniques, histologica
Histochemical And Cytochemical Methods Of Visualization
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Author : Jean-Marie Exbrayat
language : en
Publisher: CRC Press
Release Date : 2013-06-05
Histochemical And Cytochemical Methods Of Visualization written by Jean-Marie Exbrayat and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-05 with Science categories.
Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio
Visualization Of Receptors
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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 1997-02-26
Visualization Of Receptors written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-02-26 with Science categories.
A multiplicity of methods for visualization are described in Visualization of Receptors. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require and the means of implementing them. Topics discussed include localization of ligands (in vitro or in vivo), visualization of immunoreactivities of ligand and receptor proteins, detection and quantification of mRNA expression, amplification of signals, and determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.
Tietz Fundamentals Of Clinical Chemistry And Molecular Diagnostics E Book
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Author : Carl A. Burtis
language : en
Publisher: Elsevier Health Sciences
Release Date : 2014-02-26
Tietz Fundamentals Of Clinical Chemistry And Molecular Diagnostics E Book written by Carl A. Burtis and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-26 with Medical categories.
A condensed, easier-to-understand student version of the acclaimed Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 7th Edition uses a laboratory perspective in providing the clinical chemistry fundamentals you need to work in a real-world, clinical lab. Coverage ranges from laboratory principles to analytical techniques and instrumentation, analytes, pathophysiology, and more. New content keeps you current with the latest developments in molecular diagnostics. From highly respected clinical chemistry experts Carl Burtis and David Bruns, this textbook shows how to select and perform diagnostic lab tests, and accurately evaluate results. Authoritative, respected author team consists of two well-known experts in the clinical chemistry world. Coverage of analytical techniques and instrumentation includes optical techniques, electrochemistry, electrophoresis, chromatography, mass spectrometry, enzymology, immunochemical techniques, microchips, automation, and point of care testing. Learning objectives begin each chapter, providing measurable outcomes to achieve after completing the material. Key words are listed and defined at the beginning of each chapter, and bolded in the text. A glossary at the end of the book makes it quick and easy to look up definitions of key terms. More than 500 illustrations plus easy-to-read tables help you understand and remember key concepts. New chapters on molecular diagnostics include the principles of molecular biology, nucleic acid techniques and applications, and genomes and nucleic acid alterations, reflecting the changes in this rapidly evolving field. New content on clinical evaluation of methods, kidney function tests, and diabetes is added to this edition. NEW multiple-choice review questions at the end of each chapter allow you to measure your comprehension of the material. NEW case studies on the Evolve companion website use real-life scenarios to reinforce concepts.
Rna Methodologies
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Author : Robert E. Farrell Jr.
language : en
Publisher: Elsevier
Release Date : 2010-07-22
Rna Methodologies written by Robert E. Farrell Jr. and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-22 with Science categories.
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Genome Visualization By Classic Methods In Light Microscopy
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Author : Jean-Marie Exbrayat
language : en
Publisher: CRC Press
Release Date : 2000-11-27
Genome Visualization By Classic Methods In Light Microscopy written by Jean-Marie Exbrayat and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-11-27 with Science categories.
Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization. This book provides insight into several classic techniques, histological as well as histochemical, that can be used to appreciate the nucleic acid status of the cell as well as to provide an overview of RNA and DNA distribution in cells and tissues. Genome Visualization by Classic Methods in Light Microscopy begins with an introduction to DNA and RNA, followed by general visualization principles. The subsequent chapters describe: how to prepare tissues for staining; the principles, chemical formulas, and procedures for nuclear dye, fluorescent dye, and histochemical methods; directions to observe the products of the stained reactions; and more. Each protocol is presented as easy-to-follow directions and the author includes cautionary notes and points to consider. The final section provides color photographs of various tissues in which the staining method, fixative, and observations are noted. A theoretical and practical book, Genome Visualization by Classic Methods in Light Microscopy allows you to understand which technique is most useful for your particular problem. Laboratory protocols are provided for you to follow, chemical structures and principles are provided for you to understand the technique, and the book is organized so you can find the necessary information when needed. This is the essential guide to understanding and executing visualization techniques for nucleic acids.
Encyclopedia Of Bioinformatics And Computational Biology
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Author :
language : en
Publisher: Elsevier
Release Date : 2018-08-21
Encyclopedia Of Bioinformatics And Computational Biology written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-21 with Medical categories.
Encyclopedia of Bioinformatics and Computational Biology: ABC of Bioinformatics, Three Volume Set combines elements of computer science, information technology, mathematics, statistics and biotechnology, providing the methodology and in silico solutions to mine biological data and processes. The book covers Theory, Topics and Applications, with a special focus on Integrative –omics and Systems Biology. The theoretical, methodological underpinnings of BCB, including phylogeny are covered, as are more current areas of focus, such as translational bioinformatics, cheminformatics, and environmental informatics. Finally, Applications provide guidance for commonly asked questions. This major reference work spans basic and cutting-edge methodologies authored by leaders in the field, providing an invaluable resource for students, scientists, professionals in research institutes, and a broad swath of researchers in biotechnology and the biomedical and pharmaceutical industries. Brings together information from computer science, information technology, mathematics, statistics and biotechnology Written and reviewed by leading experts in the field, providing a unique and authoritative resource Focuses on the main theoretical and methodological concepts before expanding on specific topics and applications Includes interactive images, multimedia tools and crosslinking to further resources and databases
Fundamentals Of Molecular Diagnostics
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Author : David E. Bruns
language : en
Publisher: Elsevier Health Sciences
Release Date : 2007-05-25
Fundamentals Of Molecular Diagnostics written by David E. Bruns and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-25 with Medical categories.
This book offers an introduction to the newest, fastest-growing field in laboratory science. Explaining and clarifying the molecular techniques used in diagnostic testing, this text provides both entry-level and advanced information. It covers the principles of molecular biology along with genomes and nucleic acid alterations, techniques and instrumentation, and applications of molecular diagnostics. Written by leading experts, including Patrick Bossuyt, Angela Caliendo, Rossa W.K. Chiu, Kojo S.J. Elenitoba-Johnson, Andrea Ferreira-Gonzalez, Amy Groszbach, Sultan Habeebu, Doris Haverstick, Malek Kamoun, Anthony Killeen, Noriko Kusukawa, Y.M. Dennis Lo, Elaine Lyon, Gwendolyn McMillin, Christopher Price, James Versalovic, Cindy Vnencak-Jones, Victor Weedn, Peter Wilding, Thomas Williams, and Carl Wittwer, this book includes illustrations, tables, and a colorful design to make information easy to find and easy to use. A full-color, 4-page insert shows realistic images of the output for many molecular tests. Learning Objectives open each chapter with an overview of what you should achieve. Key Words are listed and defined at the beginning of each chapter, and are bolded in the text. Review Questions at the end of every chapter let you measure your comprehension. Advanced Concepts are included, but set apart from the rest of the text, for students who want a higher level of learning. Ethics boxes address ethical issues, allowing you to apply your knowledge to real-life scenarios. A glossary of all key words may be easily accessed in the back of the book.
Informational Biopolymers Of Genes And Gene Expression
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Author : R.D. Blake
language : en
Publisher: University Science Books
Release Date : 2005-03-23
Informational Biopolymers Of Genes And Gene Expression written by R.D. Blake and has been published by University Science Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-03-23 with Science categories.
This new text examines thebiophysics and biochemistry of nucleic acids and proteins, carving outthe dynamic interface between chemistry and molecular biology, and providing adetailed picture of nucleic acids and proteins, their structures, biologicalproperties, and origins and evolution.