In Situ Hybridization In Electron Microscopy
DOWNLOAD
Download In Situ Hybridization In Electron Microscopy PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get In Situ Hybridization In Electron Microscopy book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
In Situ Hybridization In Electron Microscopy
DOWNLOAD
Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 2001-01-30
In Situ Hybridization In Electron Microscopy written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-01-30 with Science categories.
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization
Hybridization Techniques For Electron Microscopy
DOWNLOAD
Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 1993-06-09
Hybridization Techniques For Electron Microscopy written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-06-09 with Science categories.
Hybridization Techniques for Electron Microscopy examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. The book features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissue method using ultrathin frozen sections, pre-embedded method, and post-embedded method using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided. Particular aspects of the techniques are examined in detail, such as preparation of tissue, pretreatment, hybridization procedures, revelation (autoradiography and immunocytology) and checking procedures, in addition to the illustration, interpretation, and discussion of methods and results. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid, and detection of messenger RNA in animals and plants. Hybridization Techniques for Electron Microscopy is an excellent reference for cytologists, cell biologists, histochemists, cytochemists, molecular endocrinologists, and neuroendocrinologists.
Electron Microscopy Of Plant Cells
DOWNLOAD
Author : C Hawes
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Electron Microscopy Of Plant Cells written by C Hawes and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Fluorescence In Situ Hybridization Fish Application Guide
DOWNLOAD
Author : Thomas Liehr
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-11-26
Fluorescence In Situ Hybridization Fish Application Guide written by Thomas Liehr and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-26 with Science categories.
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Electron Microscopy Methods And Protocols
DOWNLOAD
Author : M. A. Nasser Hajibagheri
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Electron Microscopy Methods And Protocols written by M. A. Nasser Hajibagheri and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Electron Microscopy In Viral Diagnosis
DOWNLOAD
Author : Erskine L. Palmer
language : en
Publisher: CRC Press
Release Date : 2019-07-23
Electron Microscopy In Viral Diagnosis written by Erskine L. Palmer and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-23 with Science categories.
This text on electron microscopy in viral diagnosis is an invaluable reference investigators interested in the detection of viruses or viral subcomponents in liquid preparations or in thin sectioned cells. It contains an extensive collection of negative stain, thin section, and immunolabelled electron micrographs useful for reference viral diagnosis. The salient features of the replication of many virus families are presented in schematic form and all viruses now known to cause disease in humans, including the recently recognized human retroviruses, are described in separate chapter.
Immunolabelling For Electron Microscopy
DOWNLOAD
Author : Julia M. Polak
language : en
Publisher: Elsevier Publishing Company
Release Date : 1984
Immunolabelling For Electron Microscopy written by Julia M. Polak and has been published by Elsevier Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Medical categories.
Morphology Methods
DOWNLOAD
Author : Ricardo V. Lloyd
language : en
Publisher: Springer Science & Business Media
Release Date : 2001-06-15
Morphology Methods written by Ricardo V. Lloyd and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-15 with Medical categories.
The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.
The Dictionary Of Genomics Transcriptomics And Proteomics
DOWNLOAD
Author : Guenter Kahl
language : en
Publisher: John Wiley & Sons
Release Date : 2015-05-21
The Dictionary Of Genomics Transcriptomics And Proteomics written by Guenter Kahl and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-21 with Science categories.
Now in its fifth edition and for the first time available as an electronic product with all entries cross-linked. This very successful long-seller has once again been thoroughly updated and greatly expanded. It now contains over 13,000 entries, and comprehensively covering genomics, transcriptomics, and proteomics. Each entry contains an extensive explanation, including a comprehensive listing of synonyms and acronyms, and all formulas have been redrawn to create a uniform style, while most of the figures are custom designed for this dictionary. The ultimate reference for all terms in the -omics fields.