Immunolabelling For Electron Microscopy
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Immunolabelling For Electron Microscopy
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Author : Julia M. Polak
language : en
Publisher: Elsevier Publishing Company
Release Date : 1984
Immunolabelling For Electron Microscopy written by Julia M. Polak and has been published by Elsevier Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Medical categories.
Immunolabelling For Electron Microscopy
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Author : Julia M. POLAK
language : en
Publisher:
Release Date : 1984
Immunolabelling For Electron Microscopy written by Julia M. POLAK and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with categories.
Immuno Gold Electron Microscopy In Virus Diagnosis And Research
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Author : Alexander D. Hyatt
language : en
Publisher: CRC Press
Release Date : 2024-12-20
Immuno Gold Electron Microscopy In Virus Diagnosis And Research written by Alexander D. Hyatt and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-12-20 with Science categories.
This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.
Advanced Techniques In Biological Electron Microscopy Iii
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Author : J.K. Koehler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Advanced Techniques In Biological Electron Microscopy Iii written by J.K. Koehler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This volume is a continuation of two prior books on advanced electron microscope techniques. The purpose of this series has been to provide in depth analyses of methods which are considered to be at the leading edge of electron microscopic research procedures with applications in the biological sciences. The mission of the present volume remains that of a source book for the research practitioner or advanced student, especially one already well versed in basic electron optical methods. It is not meant to provide in troductory material, nor can this modest volume hope to cover the entire spectrum of advanced technology now available in electron microscopy. In the past decade, computers have found their way into many research laboratories thanks to the enormous increase in computing power and stor age available at a modest cost. The ultrastructural area has also benefited from this expansion in a number of ways which will be illustrated in this volume. Half of the contributions discuss technologies that either directly or indirectly make extensive use of computer methods.
Biological Field Emission Scanning Electron Microscopy 2 Volume Set
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Author : Roland A. Fleck
language : en
Publisher: John Wiley & Sons
Release Date : 2019-04-29
Biological Field Emission Scanning Electron Microscopy 2 Volume Set written by Roland A. Fleck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-29 with Science categories.
The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Biological Specimen Preparation For Transmission Electron Microscopy
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Author : Audrey M. Glauert
language : en
Publisher: Princeton University Press
Release Date : 2014-07-14
Biological Specimen Preparation For Transmission Electron Microscopy written by Audrey M. Glauert and has been published by Princeton University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-14 with Science categories.
This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Electron Microscopy In Diagnostic Virology
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Author : Frances W. Doane
language : en
Publisher: CUP Archive
Release Date : 1987-01-30
Electron Microscopy In Diagnostic Virology written by Frances W. Doane and has been published by CUP Archive this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-01-30 with Medical categories.
Histochemical And Immunohistochemical Techniques
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Author : Peter H. Bach
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Histochemical And Immunohistochemical Techniques written by Peter H. Bach and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Toxicological and pharmacological effects arise when chemicals interact with biophysiological functions in discrete cell types. There is a continuing need to screen novel compounds for their potential therapeutic effects, and once these have been "discovered" to understand their molecular actions, as the basis of using such compounds safely and for rational drug design. Pharmacology now uses all of the sophisticated molecular research techniques that are available for the development of safer and more efficacious drugs. Histochemistry has been usefully applied to developing new drugs (and assessing chemical safley) and is potentially cost effective. The need to test novel substances for their potential adverse effects has raised many questions. Toxicological pathology has moved away from the cataloging of lesions towards understanding the basis of the events that underly cell injury, especially for those secondary consequences of chemical injury that lead to malignancy and chronic disease. The focal nature of toxicologic lesions de mands the use of microtechniques to provide data to help understand these questions. Histochemistry is under-utilized, but offers one of the key ap proaches necessary to address the problem of understanding interactions between a cell population and a chemical, the modulation of cellular biochem istryor the presence of a lesion in a test animal can be rationalised in terms of species differences that have no relevance to man as opposed to those that are of clinical significance or represent a warning of dire consequences to man.
Electron Microscopy In Microbiology
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Author :
language : en
Publisher: Academic Press
Release Date : 1988-10-01
Electron Microscopy In Microbiology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-10-01 with Science categories.
This volume of this acclaimed series deals with electron microscopic techniques applied for the elucidation of microbial structures and structure-function relationships at cellular, sub-cellular, and macromolecular levels. Many of the recent findings on ultrastructural features of microorganisms have been obtained with newly developed methods, though classical approaches have not lost their validity. Therefore, both conventional and new methods have been incorporated into this volume. The topics dealt with are meaningful not only in bacterial cytology but also in physiology, enzymology, biochemistry, and molecular biology, and include aspects of medical and biotechnological application.
Electron Microscopic Immunocytochemistry
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Author : Julia M. Polak
language : en
Publisher:
Release Date : 1992
Electron Microscopic Immunocytochemistry written by Julia M. Polak and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.
Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened the range of applications. It presents the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of fields, including biomedical research and diagnostic pathology. The book begins with an overview of EM immunocytochemical procedures and of applications in histopathology. The three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumors, renal disease, neuropathology, dermatology, and microbiology. The emphasis throughout this detailed volume is on providing practical guidance to scientists and clinicians who may be using the technique for the first time.