Enzyme Engineering And Evolution Specific Enzyme Applications
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Enzyme Engineering And Evolution Specific Enzyme Applications
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Author :
language : en
Publisher: Academic Press
Release Date : 2020-09-15
Enzyme Engineering And Evolution Specific Enzyme Applications written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-15 with Science categories.
Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series
Directed Enzyme Evolution
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Author : Frances H. Arnold
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Directed Enzyme Evolution written by Frances H. Arnold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum “you get what you screen for. ” The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.
Enzymes In Organic Synthesis
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Author : Ruth Porter
language : en
Publisher: John Wiley & Sons
Release Date : 2009-09-14
Enzymes In Organic Synthesis written by Ruth Porter and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-14 with Science categories.
The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Enzyme Technology
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Author : Ashok Pandey
language : en
Publisher:
Release Date : 2005-01-01
Enzyme Technology written by Ashok Pandey and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-01-01 with Enzymes categories.
Enzymes are now a material well established in the field of biotechnology.This book is a unique resource for state-of-the-art research findings onbiotechnological innovations in the area of industrial and therapeuticenzymes, and special-function and extreme-nature enzymes such asribozymes, therozymes, cold-adapted enzymes, etc, covering all aspects such as the producingmicro-organisms, their mode of cultivation, downstream processing and applications. It providesgreat deal of information on potential of enzymes for their commercial exploitation. It alsoprovides unique insight for a researcher who intends to enter the world of enzymes and gives upto-date on advances on various topics in the area of enzymes for the experts. The vitalinformations have been organized in an easy-to-use format that lets reader become familiar withhighlights of the most relevant topics and includes photographs, figures, and tables. The topicsincluded in the book have been classified under following main sections:" General and fundamental aspects " Design of bioreactors " Special enzymes " Industrial enzymes" Enzymes applications.The book has 36 high quality contributions from internationally recognized experts from Europe,Americas, Asia and Australia. Often enzyme technology is best described as the technologyassociated with the application of enzymes as the tools of industry, agriculture and medicine, andthis is what has been exactly focussed and covered in this book.The book is a vital resource for practitioners, researchers, and academical working in the areas ofbiotechnology, biochemical engineering, bioprocess technology, enzyme technology,biochemistry and applied microbiology.
Enzyme Engineering
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Author : James Samuelson
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Enzyme Engineering written by James Samuelson and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Science categories.
This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases.
Enzyme Engineering
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Author : Manfred T. Reetz
language : en
Publisher: John Wiley & Sons
Release Date : 2023-01-09
Enzyme Engineering written by Manfred T. Reetz and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-09 with Science categories.
Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity. The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work. Readers will also find: A thorough introduction to directed evolution and rational design as protein engineering techniques Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry Treatments of artificial enzymes as promiscuous catalysts Various lessons learned from semi-rational and rational directed evolution A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.
Enzymatic Processes For Food Valorization
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Author : Monica L. Chavez Gonzalez
language : en
Publisher: Elsevier
Release Date : 2024-06-23
Enzymatic Processes For Food Valorization written by Monica L. Chavez Gonzalez and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-23 with Technology & Engineering categories.
Enzymatic Processes for Food Valorization describes the most recent research in the field of catalysis for food valorization, revealing the impact of the implementation of enzymatic catalysis in the different stages that make up the production processes. Sections review advances in food processing using enzymes, explore the use of enzymes on by-products for the release of compounds of interest, and show recent trends in biocatalysis and its application in the food industry. Written by a team of international experts, this is an invaluable guide for professionals in the area of enzyme technology applied in the food industry, as well as technicians and scientists involved in the use of enzymes on food waste for the valorization and/or recovery of compounds. - Brings updated content on trends in enzymatic processes for food valorization - Presents the main enzymes used in food processing and technology to improve organoleptic and quality attributes - Includes the application of enzymes for the valorization of by-products generated during food processing for an eventual recovery of bioactive - Explores how food by-products can be used as fermentation substrates for the production of enzymes of industrial interest
Directory Of Therapeutic Enzymes
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Author : Barry M. McGrath
language : en
Publisher: CRC Press
Release Date : 2005-08-12
Directory Of Therapeutic Enzymes written by Barry M. McGrath and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-08-12 with Medical categories.
Carefully crafted to provide tightly focused and authoritative information, the Directory of Therapeutic Enzymes covers all approved therapeutic enzymes currently used in medicine. Written mainly by industry experts, the book includes information sourced directly from the company that developed or manufactured the product. It explores major
Enzymes And Their Biotechnological Applications
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Author : Pabulo H. Rampelotto
language : en
Publisher: MDPI
Release Date : 2018-10-02
Enzymes And Their Biotechnological Applications written by Pabulo H. Rampelotto and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-02 with Medical categories.
This book is a printed edition of the Special Issue "Enzymes and Their Biotechnological Applications" that was published in Biomolecules
Engineering Biology For Microbial Biosynthesis Of Plant Derived Bioactive Compounds
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Author : Yongjun Wei
language : en
Publisher: Elsevier
Release Date : 2024-03-01
Engineering Biology For Microbial Biosynthesis Of Plant Derived Bioactive Compounds written by Yongjun Wei and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-03-01 with Science categories.
Many plant-derived bioactive compounds are the foundation effective drugs to cure diseases. Usually, the bioactive compounds in plant biomass are low, and the extraction of bioactive compounds from plants is not eco-friendly, which limits their application in drugs and other industries. With the development of synthetic biology and green biomanufacturing, the biosynthesis of complex bioactive compounds is possible. However, the engineering fundamentals for microbial biosynthetic of plant-derived bioactive compounds is vast. Engineering biology for microbial biosynthesis of plant-derived bioactive compounds summarizes the engineering fundamental biotechnologies for microbial production of plant-derived bioactive compounds, allowing this content to be easily accessed. Coverage includes summaries of key achievements, identification of challenges, current approaches, tips and tricks, and helpful insights into critical bottlenecks. With a comprehensive coverage of the field, Engineering biology for microbial biosynthesis of plant-derived bioactive compounds is of great interest to undergraduate and graduate students, scientists, researchers as well as engineers who work on the microbial production of plant-derived bioactive compounds. - Provides valuable information for engineers, scientists, and industrial researchers who want to obtain microbial strains with the ability of plant-derived bioactive compound production - Guides microbial production of plant-derived bioactive compounds at the laboratory and industrial levels - Ideal resource for anyone who works on the microbial production of plant-derived bioactive compounds