Enzyme Immobilization
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Enzyme Immobilization
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Author : Rowan Austin
language : en
Publisher: Scientific e-Resources
Release Date : 2018-12-19
Enzyme Immobilization written by Rowan Austin and has been published by Scientific e-Resources this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-12-19 with categories.
An enzyme is a protein, or protein complex, that catalyses a chemical reaction. Like any catalyst, enzymes work by lowering the activation energy of a reaction, thus allowing the reaction to proceed to its steady state or completion much faster than it otherwise would; the enzyme remains unaltered by the completed reaction and can therefore continue catalysis. An immobilized enzyme is an enzyme attached to an inert, insoluble material-such as calcium alginate. This can provide increased resistance to changes in conditions such as pH or temperature. It also lets enzymes be held in place throughout the reaction, following which they are easily separated from the products and may be used again-a far more efficient process and so is widely used in industry for enzyme catalysed reactions. An alternative to enzyme immobilization is whole cell immobilization. Carrier matrices for enzyme immobilisation by adsorption and covalent binding must be chosen with care. The manufacture of high-valued products on a small scale may allow the use of relatively expensive supports and immobilisation techniques whereas these would not be economical in the large-scale production of low added-value materials. A substantial saving in costs occurs where the carrier may be regenerated after the useful lifetime of the immobilised enzyme. This book is simple protocols for the immobilization of enzymes and cells that could be useful for application at industrial scale, novel protocols for immobilization in the future, and new chemical reactors able to overcome the limitations of a number of immobilized derivatives.
Immobilized Enzymes
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Author : Michael D. Trevan
language : en
Publisher: John Wiley & Sons
Release Date : 1980
Immobilized Enzymes written by Michael D. Trevan and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Science categories.
Enzyme Immobilization
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Author : Alka Dwevedi
language : en
Publisher: Springer
Release Date : 2016-09-15
Enzyme Immobilization written by Alka Dwevedi and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-15 with Science categories.
This book covers the latest developments in enzyme immobilization with its wide applications, such as for industry, agriculture, medicine, and the environment. Topics covered include basics of enzyme immobilization, its implication in therapeutics and disease diagnostics, and its significance in solving environmental problems. This is an ideal book for researchers, graduate and postgraduate students, as well as scientists in industry, agriculture and health sectors. This book is a complete summary of enzyme immobilization and also thoroughly covers all the latest research. This book covers: The last one-hundred years of innovative research done in enzyme immobilization Recent developments in immobilization techniques, such as types of matrices, immobilization methods, and linking agents, as well as enzyme immobilization without any matrices and its properties The physiological and industrial significance of enzymes from plants and the implementation of immobilized enzymes in the treatment of waste water and polluted air Biomedical and bioanalytical applications of immobilized enzymes
Carrier Bound Immobilized Enzymes
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Author : Linqiu Cao
language : en
Publisher: John Wiley & Sons
Release Date : 2006-05-12
Carrier Bound Immobilized Enzymes written by Linqiu Cao and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-05-12 with Science categories.
The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes. A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.
Immobilization Of Enzymes And Cells
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Author : José M. Guisán
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Immobilization Of Enzymes And Cells written by José M. Guisán and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.
Polymeric Supports For Enzyme Immobilization
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Author : Alka Dwevedi
language : en
Publisher: Academic Press
Release Date : 2021-06-22
Polymeric Supports For Enzyme Immobilization written by Alka Dwevedi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-06-22 with Science categories.
Enzyme immobilization has been approached for finding solutions for various critical problems associated with industries, medicine, environment, agriculture, etc. Especially since last decades, several innovative researches have come up to look for enhancing catalytic efficiency, reusability of immobilized enzyme and longer stability by introducing range of immobilizing supports, supports modifiers through introduction of several chemical agents (non-toxic) and adopting innovative enzyme immobilization methods. In the present book, polymeric supports have been focussed for enzyme immobilization, especially due to their versatility in immobilizing different enzymes for different large scale enzyme reactors to be used for several applications. Especially, polymers can be modified according to applications and enzyme properties which have made it supports of choice for all several enzyme based applications. Polymeric Supports for Enzyme Immobilization: Opportunities and Applications offers in-depth discussions of known polymeric enzyme support materials, reaction processes, and optimized methods to enhance enzyme immobilization. Case-based chapters examine methods of enzyme immobilization onto various polymeric supports, their surface chemistry and physical morphology followed by implementation of polymers based immobilized enzymes in various applications, viz. medicine, environment, industries, clean energy, disease diagnosis, drug delivery etc. This book has prime focus to allow several researchers across the world to provide updated technological details and incite to contribute more innovative work in coming years to find solutions to several critical problems. Offers an in-depth, case-driven discussion of known polymeric enzyme support materials, associated reaction processes, and methods to enhance enzyme immobilization Provides optimal strategies for various enzymes, processes, and applications, considering the enzyme itself, substrate, and available support properties Provides complete details on applications of polymeric based immobilized enzymes in various applications ranging from chemical; or pharmaceutical synthesis, food processing, bioremediation, industrial catalysis, etc.
Immobilized Enzyme Principles
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Author : Lemuel B. Wingard
language : en
Publisher: Elsevier
Release Date : 2014-06-28
Immobilized Enzyme Principles written by Lemuel B. Wingard and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-28 with Technology & Engineering categories.
Applied Biochemistry and Bioengineering, Volume 1: Immobilized Enzyme Principles focuses on the utilization of immobilization techniques for the study and application of enzyme catalysts in a variety of potential end-uses. This book emphasizes the preparation of enzyme-support systems, effects caused by the concurrent phenomena of enzyme-catalyzed reaction kinetics and mass transfer resistances, and how these reactions are incorporated into the design of enzyme-catalyzed reactor systems. The magnitude of the perturbation of the apparent kinetic parameters of an immobilized enzyme that could serve in principle as a measure of the effective concentrations of substrate, modifier, or inhibitor at the site of the enzymic reaction is also explained. This volume is recommended for biological scientists and engineers, as well as researchers interested in the biochemical common denominator that causes the interaction of engineering practice and biological sciences for technological development.
Immobilized Enzymes
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Author : Oskar R. Zaborsky
language : en
Publisher:
Release Date : 1973
Immobilized Enzymes written by Oskar R. Zaborsky and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with Science categories.
Biocatalyst Immobilization
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Author : Maria Lujan Ferreira
language : en
Publisher: Academic Press
Release Date : 2022-11-12
Biocatalyst Immobilization written by Maria Lujan Ferreira and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-12 with Science categories.
Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends. Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization Features chapter contributions from international leaders in the field
Immobilized Enzymes In Food And Microbial Processes
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Author : Alfred Olson
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Immobilized Enzymes In Food And Microbial Processes written by Alfred Olson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
In the last five years the potential value of immobilized enzymes has captured the imagination of an increasing number of scientists and engineers. The concept of being able to create an immobilized derivative of an enzyme which has long-term stability and is able to be recovered and reused is fascinating, to say the least. Since the industrial application of enzymes has been mostly in the food and microbial process industry it is not surprising that many of the applications of immobilized enzymes considered for commercial development fall within the area of this industry. It is for this reason that we organized a symposium on immobilized enzymes for the 166th National Meeting of the American Chemical Society. Appropriately, the symposium was jointly sponsored by the Division of Agricultural and Food Chemistry and the Division of Microbial Chemistry and Technology. Although there were at least half a dozen symposia at other meetings on various aspects of immobilized enzyme technology in the preceding ten months none had specifically addressed themselves to food and microbial processes and none had been held at a meeting such as the National ACS gathering, which is not only large but multidisciplinary. The enthusiastic response to this symposium prompted us, at the invita tion of Plenum Press, to publish the proceedings of this symposium.