Enzyme Immunoassay
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Enzyme Immunoassays
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Author : S.S. Deshpande
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Enzyme Immunoassays written by S.S. Deshpande and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.
Enzyme Immunoassay
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Author : Edward T. Maggio
language : en
Publisher: CRC Press
Release Date : 2018-01-18
Enzyme Immunoassay written by Edward T. Maggio and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-18 with Health & Fitness categories.
The purpose of this book is to focus attention on some of these ideas and concepts. In doing so, it has captured a glimpse of the past and it attempts a projection of the future, but mostly it reveals an overview of the field as it exists as the present time. It aims to serve to spawn further growth in ideas and encourage applications to increasingly broader segments of both clinical and general analytical chemistry fields.
Practice And Theory Of Enzyme Immunoassays
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Author : P. Tijssen
language : en
Publisher: Elsevier
Release Date : 1985-08-01
Practice And Theory Of Enzyme Immunoassays written by P. Tijssen and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-08-01 with Medical categories.
Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.
Enzyme Mediated Immunoassay
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Author : H.M. Lenhoff
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Enzyme Mediated Immunoassay written by H.M. Lenhoff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.
Enzyme Immunoassay
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Author : Edward T. Maggio
language : en
Publisher:
Release Date : 1980
Enzyme Immunoassay written by Edward T. Maggio and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with categories.
Ultrasensitive And Rapid Enzyme Immunoassay
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Author : E. Ishikawa
language : en
Publisher: Elsevier
Release Date : 1999-11-11
Ultrasensitive And Rapid Enzyme Immunoassay written by E. Ishikawa and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-11-11 with Science categories.
Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as well as ultrasensitive enzyme immunoassays. Ultrasensitive and Rapid Enzyme Immunoassay, with its clear presentation and up-to-date information, will be essential to scientists in pharmaceutical companies and in applied research.
Nonisotopic Immunoassay
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Author : That T. Ngo
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Nonisotopic Immunoassay written by That T. Ngo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.
Quantitative Enzyme Immunoassay
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Author : Eva Engvall
language : en
Publisher: Blackwell Publishers
Release Date : 1978
Quantitative Enzyme Immunoassay written by Eva Engvall and has been published by Blackwell Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978 with Enzyme-linked immunosorbent assay categories.
Principles And Practice Of Immunoassay
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Author : Christopher P. Price
language : en
Publisher: Springer
Release Date : 1991-06-18
Principles And Practice Of Immunoassay written by Christopher P. Price and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-06-18 with Medical categories.
Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.
The Enzyme Linked Immunosorbent Assay Elisa
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Author : A. Voller
language : en
Publisher:
Release Date : 1979
The Enzyme Linked Immunosorbent Assay Elisa written by A. Voller and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with Medical categories.