Enzyme Linked Immunosorbent Assay
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Elisa
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Author : John R. Crowther
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Elisa written by John R. Crowther and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Enzyme Linked Immunosorbent Assay Elisa
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Author : Samira Hosseini
language : en
Publisher: Springer
Release Date : 2017-12-30
Enzyme Linked Immunosorbent Assay Elisa written by Samira Hosseini and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-30 with Technology & Engineering categories.
This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
Enzyme Linked Immunosorbent Assay
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Author : Sarika Mohan
language : en
Publisher: Elsevier Health Sciences
Release Date : 2019-10-31
Enzyme Linked Immunosorbent Assay written by Sarika Mohan and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-31 with Medical categories.
Enzyme Linked Immunosorbent Assay
The Enzyme Linked Immunosorbent Assay Elisa A Review Of Recent Developments With Abstracts Of Microplate Applications 2 1980
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Author : Alister Voller
language : en
Publisher:
Release Date : 1980
The Enzyme Linked Immunosorbent Assay Elisa A Review Of Recent Developments With Abstracts Of Microplate Applications 2 1980 written by Alister Voller and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Immunoenzyme technique categories.
The Elisa Enzyme Linked Immunosorbent Assay In Veterinary Research And Diagnosis
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Author : R.C. Wardley
language : en
Publisher: Springer Science & Business Media
Release Date : 1982-11-30
The Elisa Enzyme Linked Immunosorbent Assay In Veterinary Research And Diagnosis written by R.C. Wardley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982-11-30 with Medical categories.
Proceedings of a Meeting held at the University of Surrey, Guildford, U.K., September 15-17, 1981
The Enzyme Linked Immunosorbent Assay Elisa
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Author : A. Voller
language : en
Publisher:
Release Date : 1979
The Enzyme Linked Immunosorbent Assay Elisa written by A. Voller and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with Medical categories.
Enzyme Linked Immunosorbent Assay Systems And Food Analysis
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Author : Assoc. Prof. Dr Nihat TELLİ
language : en
Publisher: Duvar Yayınları
Release Date : 2024-05-30
Enzyme Linked Immunosorbent Assay Systems And Food Analysis written by Assoc. Prof. Dr Nihat TELLİ and has been published by Duvar Yayınları this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-05-30 with Reference categories.
The Enzyme Linked Immunosorbent Assay Elisa
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Author : A. Voller
language : en
Publisher:
Release Date : 1977
The Enzyme Linked Immunosorbent Assay Elisa written by A. Voller and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977 with Enzymatic analysis categories.
Elisa
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Author : Robert Hnasko
language : en
Publisher: Humana
Release Date : 2015-07-10
Elisa written by Robert Hnasko and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-07-10 with Medical categories.
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Elisa Enzyme Linked Immunsorbent Assay Practical Report
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Author : Yumna Mubashir
language : de
Publisher:
Release Date : 2015-05-07
Elisa Enzyme Linked Immunsorbent Assay Practical Report written by Yumna Mubashir and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-07 with categories.
Essay aus dem Jahr 2015 im Fachbereich Biologie - Humanbiologie, Sprache: Deutsch, Abstract: The Enzyme-Linked Immunosorbent assay (ELISA) is a test that is conducted to identify the substance under study with the help of antibodies and changing color elements. It is the "wet-lab" oriented analytic biochemistry assay that consume enzyme immunoassay to detect the existence of a substance (antigen) in a liquid sample. It is of great help in medicine and plant pathology as a diagnostic tool and quality control check. ELISA test involves one antibody according to the need of the test, where the antigen is immobilized on the solid plate. The method is done specifically and nonspecifically. The report involves the description and construction of two different ELISAs.