Enzyme Linked Immunosorbent Assay Systems And Food Analysis
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Enzyme Linked Immunosorbent Assay Systems And Food Analysis
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Author : Assoc. Prof. Dr Nihat TELLİ
language : en
Publisher: Duvar Yayınları
Release Date : 2024-05-30
Enzyme Linked Immunosorbent Assay Systems And Food Analysis written by Assoc. Prof. Dr Nihat TELLİ and has been published by Duvar Yayınları this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-05-30 with Reference categories.
Enzyme Linked Immunosorbent Assay Elisa
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Author : Samira Hosseini
language : en
Publisher: Springer
Release Date : 2017-12-30
Enzyme Linked Immunosorbent Assay Elisa written by Samira Hosseini and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-30 with Technology & Engineering categories.
This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
Techniques To Measure Food Safety And Quality
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Author : Mohidus Samad Khan
language : en
Publisher: Springer Nature
Release Date : 2021-09-22
Techniques To Measure Food Safety And Quality written by Mohidus Samad Khan and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-22 with Technology & Engineering categories.
This book addresses the basic understanding of food contaminants and their sources, followed by the techniques to measure food safety and quality. It is divided into four parts: Part A - sources of contaminants in foods, their associated health risks, and integrated management and alternative options to minimize contaminants; Part B - Technological assessment of conventional methods and selected advanced methods for the detection, identification and enumeration of microbial contaminates; Part C - Technological assessment of different chemical measurements techniques; and Part D – Technological assessment of different instrumental techniques to assess sensory properties of foods. Food safety is a growing concern due to the increase in food-borne illnesses caused by food adulteration, excessive use of pesticides, use of chemical preservatives and artificial fruit ripening agents, microbial contaminations, and improper food handling. Chemical contaminants in food could be transferred from environmental or agrochemical sources, personal care products, and other by-products of water disinfects. In addition, microbial food safety can be threatened due to the presence of many pathogens, such as Salmonella, Escherichia coli, Clostridium botulinum, Staphylococcus aureus, and Listeria monocytogenes in foods. Globally, strict regulations are imposed to limit the potential contaminants in foods. Development of accurate, rapid, and inexpensive approaches to test food contamination and adulteration would be highly valued to ensure global food safety. There are existing processes to ensure safety of food products from chemical and microbial contaminants. Apart from the existing measurement technologies, varieties of new techniques are also being emerged and these could be potential to ensure food safety and quality. In addition to chemical and microbial properties, sensory properties such as texture, mouth feel, flavor, and taste, are among the most important attributes of food products to ensure their acceptability by consumers. Two approaches are available to evaluate sensory properties of food products, namely subjective and objective analyses. The responses are perceived by all five senses: smell, taste, sight, touch, and hearing. The approach used in sensory evaluation varies depending on the types of foods and the ultimate goal of the testing. Sensory attributes are the most important quality parameters after ensuring the safety of foods.
Matrix Effects On The Detection Of Milk And Peanut Residues By Enzyme Linked Immunosorbent Assays Elisa
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Author : Abigail S. Burrows
language : en
Publisher:
Release Date : 2016
Matrix Effects On The Detection Of Milk And Peanut Residues By Enzyme Linked Immunosorbent Assays Elisa written by Abigail S. Burrows and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Food matrices are complex systems of lipids, carbohydrates, and proteins in which interactions between matrix components and allergenic proteins are known to have negative effects on the recovery of allergens when analyzed by ELISA. The purpose of this study was to first evaluate the recovery of milk and peanut residues from multiple food matrices and mixes and to secondly evaluate the use of a modified extraction protocol, sequential extractions, on the recovery of milk and peanut allergens. Pastry dough matrices and pastry dough mixes incurred with milk were prepared at varying concentrations of flour and evaluated for recovery of NFDM. Secondly, a series of samples were prepared with increasing flour concentrations (wheat, corn, rice, soy flour) while maintaining a constant allergen (milk or peanut) concentration. Evaluation of sequential extractions was done on pastry matrices (wheat, corn, or rice flour) incurred with milk or peanut. Two matrix types, raw dough and baked matrices were analyzed for recovery. Recovery of NFDM was reduced in wet pastry dough matrices in comparison to dry pastry dough mixes, indicating that the formation of a food matrix influences the detection of allergens. In concentration mixes, upon the addition of each flour type, the recovery of milk residues decreased as the concentration of flour increased whereas the recovery of peanut residues was not affected by the increasing concentrations of flour. The implementation of sequential extractions yielded additional soluble protein from all matrices analyzed. Interestingly, ELISA detectable protein was only extracted from raw dough matrices. No detectable allergenic protein was extracted from baked pastry matrices. The formation of a food matrix reduces the detection of milk allergens and reduced recoveries of milk allergens were observed with both glutinous and non-glutinous flour mixes. Peanut residues are less affected in sample mixes of different flour types. The use of a modified extraction procedure improved the recovery of soluble protein (all matrices) and allergenic protein (in raw matrices only).
Principles And Techniques Of Food Analysis
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Author : Samuel Ford
language : en
Publisher: Syrawood Publishing House
Release Date : 2023-09-19
Principles And Techniques Of Food Analysis written by Samuel Ford and has been published by Syrawood Publishing House this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-19 with Technology & Engineering categories.
Food analysis is a discipline that is concerned with the development, application and study of analytical procedures for characterizing the properties and constituents of food items. It aims to provide information about different properties of food such as composition, structure, physicochemical properties, and sensory attributes. Several techniques are used to carry out food analysis. Mass spectrometry (MS) is one of the most prominent food analysis techniques used extensively for the study of different food qualities such as nutraceutical active compounds, nutritional value and micronutrients. Enzyme-linked immunosorbent assay (ELISA) is another analytical procedure commonly used to detect hidden allergenic proteins in food products such as hormones, antibodies and peptides. Lateral flow device (LFD) testing is an immunological assay technique used to detect gluten in products, with an antigen-antibody reaction used to identify the protein. This book provides comprehensive insights into the basic concepts and techniques of food analysis. With its detailed analyses and data, it will prove immensely beneficial to professionals and students involved in food analysis at various levels.
Nanosensing And Bioanalytical Technologies In Food Quality Control
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Author : Pranjal Chandra
language : en
Publisher: Springer Nature
Release Date : 2022-03-25
Nanosensing And Bioanalytical Technologies In Food Quality Control written by Pranjal Chandra and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-25 with Technology & Engineering categories.
This book reviews applications of nanomaterial and nanodevices in the food industry. It also discusses the advanced bioanalytical techniques, including Enzyme-Linked Immunosorbent Assay (ELISA), immunoanalytical techniques, monoclonal antibody-based immunological techniques for detecting food adulterations and allergens. It comprehensively covers electrode modification and nano-engineered fabrication of biosensors to enhance their functionalities for utilization in food industries. The book highlights the utilization of nanobiosensors for food safety and quality analysis, such as detection of toxin, food-borne pathogen, allergen, and evaluation of toxicity. Further, it also summarizes the recent advances in nanodevices such as nano-systems, nano-emulsions, nanopesticide, nanocapsule and their applications in the food industry. Lastly, it covers nanomaterial-based sensors for drug analysis in diverse matrices. It serves as an invaluable source of information for professionals, researchers, academicians, and students related to food science and technology.
Rapid Food Analysis And Hygiene Monitoring
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Author : Pierre-Jean Raugel
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Rapid Food Analysis And Hygiene Monitoring written by Pierre-Jean Raugel and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Technology & Engineering categories.
PROF. DR. ELKE ANKlAM Food control is essential for consumer protection. Due to the fact that agricul ture and food technology have increased rapidly in the past the analytical prob lems concerning food have become more complex. The consumer expects com petitively priced food of consistently high quality. The main consumer concerns are food safety and food quality including authenticity proof. Many national or international official, validated, reference or routine methods are existing. Food be performed rapidly especially in the fields of microbiological control has to contamination and customs control. This handbook describes many kits, instruments and systems used for quality control of food. The tools listed are not only restricted to validated analytical methods but are also foreseen for routine and screening methods. In addition, an address list of manufacturers, distributors and sales agencies is given to gether with a list and information concerning selected expert laboratories. In this edition, emphasis is put on validation procedures of three organizations (AOAC, AFNOR and Microval). The purpose of this book is to facilitate the purchase and use of kits needed for food analysis and is therefore an important help for food analysts.
Development Of Electrochemical Antibody Based And Enzymatic Assays For Mycotoxin Analysis In Food
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Author : Soraya Lisanne Riedel
language : en
Publisher:
Release Date : 2023
Development Of Electrochemical Antibody Based And Enzymatic Assays For Mycotoxin Analysis In Food written by Soraya Lisanne Riedel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.
Electrochemical methods are promising to meet the demand for easy-to-use devices monitoring key parameters in the food industry. Many companies run own lab procedures for mycotoxin analysis, but it is a major goal to simplify the analysis. The enzyme-linked immunosorbent assay using horseradish peroxidase as enzymatic label, together with 3,3',5,5' tetramethylbenzidine (TMB)/H2O2 as substrates allows sensitive mycotoxin detection with optical detection methods. For the miniaturization of the detection step, an electrochemical system for mycotoxin analysis was developed. To this end, the electrochemical detection of TMB was studied by cyclic voltammetry on different screen-printed electrodes (carbon and gold) and at different pH values (pH 1 and pH 4). A stable electrode reaction, which is the basis for the further construction of the electrochemical detection system, could be achieved at pH 1 on gold electrodes. [...].
Enzyme Immunoassay
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Author : Eiji Ishikawa
language : en
Publisher: Igaku-Shoin Medical Publishers
Release Date : 1981
Enzyme Immunoassay written by Eiji Ishikawa and has been published by Igaku-Shoin Medical Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with Science categories.
Biosensors Based On Sandwich Assays
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Author : Fan Xia
language : en
Publisher: Springer
Release Date : 2018-01-09
Biosensors Based On Sandwich Assays written by Fan Xia and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-09 with Science categories.
This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.