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Evolutionary Methods In Biotechnology


Evolutionary Methods In Biotechnology
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Evolutionary Methods In Biotechnology


Evolutionary Methods In Biotechnology
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Author : Susanne Brakmann
language : en
Publisher: John Wiley & Sons
Release Date : 2006-03-06

Evolutionary Methods In Biotechnology written by Susanne Brakmann and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.


Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed. From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems. Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution. As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms. In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.



Biotechnology Of Microbial Enzymes


Biotechnology Of Microbial Enzymes
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Author : Goutam Brahmachari
language : en
Publisher: Academic Press
Release Date : 2016-07-21

Biotechnology Of Microbial Enzymes written by Goutam Brahmachari and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-07-21 with Science categories.


Biotechnology of Microbial Enzymes: Production, Biocatalysis and Industrial Applications provides a complete survey of the latest innovations on microbial enzymes, highlighting biotechnological advances in their production and purification along with information on successful applications as biocatalysts in several chemical and industrial processes under mild and green conditions. Applications of microbial enzymes in food, feed, and pharmaceutical industries are given particular emphasis. The application of recombinant DNA technology within industrial fermentation and the production of enzymes over the last 20 years have produced a host of useful chemical and biochemical substances. The power of these technologies results in novel transformations, better enzymes, a wide variety of applications, and the unprecedented development of biocatalysts through the ongoing integration of molecular biology methodology, all of which is covered insightfully and in-depth within the book. - Features research on microbial enzymes from basic science through application in multiple industry sectors for a comprehensive approach - Includes information on metabolic pathway engineering, metagenomic screening, microbial genomes, extremophiles, rational design, directed evolution, and more - Provides a holistic approach to the research of microbial enzymes



Fundamentals And Application Of New Bioproduction Systems


Fundamentals And Application Of New Bioproduction Systems
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Author : An-Ping Zeng
language : en
Publisher: Springer
Release Date : 2014-07-08

Fundamentals And Application Of New Bioproduction Systems written by An-Ping Zeng and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-08 with Science categories.


Printing Peptide Arrays with a Complementary Metal Oxide Semiconductor Chip, by Felix F. Loeffler, Yun-Chien Cheng, Bastian Muenster, Jakob Striffler, Fanny C. Liu, F. Ralf Bischoff, Edgar Doersam, Frank Breitling, Alexander Nesterov-Mueller. Protein Engineering as a Tool for the Development of Novel Bio production Systems, by Uwe T. Bornscheuer. Compartmentalization and Metabolic Channeling for Multienzymatic Biosynthesis: Practical Strategies and Modeling Approaches, by U. Jandt, C. You, Y. H.-P. Zhang, A.-P. Zeng. Cell-Free Systems: Functional Modules for Synthetic and Chemical Biology, by Marlitt Stech, Andreas K. Brödel, Robert B. Quast, Rita Sachse, Stefan Kubick. New Bio production Systems: From Molecular Circuits to Novel Reactor Concepts in Cell-Free Biotechnology, by Steffen Rupp. Cell-free Biosystems in the Production of Electricity and Bioenergy, by Zhiguang Zhu, Tsz Kin Tam, Y.-H. Percival Zhang. In Vitro Multi enzymatic Reaction Systems for Biosynthesis, by Inés Ardao, Ee Taek Hwang, An-Ping Zeng. Directed Multistep Biocatalysis Using Tailored Permeabilized Cells, by Steffen Krauser, Christian Weyler, Lisa Katharina Blaß, Elmar Heinzle.



Enzyme Engineering And Evolution General Methods


Enzyme Engineering And Evolution General Methods
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Author :
language : en
Publisher: Academic Press
Release Date : 2020-09-05

Enzyme Engineering And Evolution General Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-05 with Science categories.


This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series



Asymmetric Organic Synthesis With Enzymes


Asymmetric Organic Synthesis With Enzymes
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Author : Vicente Gotor
language : en
Publisher: John Wiley & Sons
Release Date : 2008-09-08

Asymmetric Organic Synthesis With Enzymes written by Vicente Gotor and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-08 with Science categories.


Perfect for biochemists, synthetic and organic chemists, this book covers all important reactions, including C-C coupling reactions, oxidation reactions and many more. Divided into two parts, the first section on methodology presents new innovative methods for enzymatic catalysis optimization, including such new trends as medium engineering, directed evolution and computer-aided prediction of enantioselectivity. The second and main section deals with applications to synthesis, showing important reaction types and their applications. Only those reactions with very high selectivity are presented, allowing readers to improve their own reaction yields.



Physics Of Self Organization And Evolution


Physics Of Self Organization And Evolution
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Author : Werner Ebeling
language : en
Publisher: John Wiley & Sons
Release Date : 2011-09-19

Physics Of Self Organization And Evolution written by Werner Ebeling and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-19 with Science categories.


This thoroughly updated version of the German authoritative work on self-organization has been completely rewritten by internationally renowned experts and experienced book authors to also include a review of more recent literature. It retains the original enthusiasm and fascination surrounding thermodynamic systems far from equilibrium, synergetics, and the origin of life, representing an easily readable book and tutorial on this exciting field. The book is unique in covering in detail the experimental and theoretical fundamentals of self-organizing systems as well as such selected features as random processes, structural networks and multistable systems, while focusing on the physical and theoretical modeling of natural selection and evolution processes. The authors take examples from physics, chemistry, biology and social systems, and include results hitherto unpublished in English. The result is a one-stop resource relevant for students and scientists in physics or related interdisciplinary fields, including mathematical physics, biophysics, information science and nanotechnology.



Protein Engineering Handbook


Protein Engineering Handbook
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Author : Stefan Lutz
language : en
Publisher: John Wiley & Sons
Release Date : 2012-07-31

Protein Engineering Handbook written by Stefan Lutz and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-31 with Science categories.


Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.



Reprogramming Microbial Metabolic Pathways


Reprogramming Microbial Metabolic Pathways
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Author : Xiaoyuan Wang
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-10-19

Reprogramming Microbial Metabolic Pathways written by Xiaoyuan Wang and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-10-19 with Medical categories.


Metabolic engineering has been developed over the past 20 years to become an important tool for the rational engineering of industrial microorganisms. This book has a particular interest in the methods and applications of metabolic engineering to improve the production and yield of a variety of different metabolites. The overall goal is to achieve a better understanding of the metabolism in different microorganisms, and provide a rational basis to reprogram microorganisms for improved biochemical production.



Bio Inspired Catalysts


Bio Inspired Catalysts
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Author : Thomas R. Ward
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-02-05

Bio Inspired Catalysts written by Thomas R. Ward and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-02-05 with Science categories.


In order to meet the ever-increasing demands for enantiopure compounds, heteroge- ous, homogeneous and enzymatic catalysis evolved independently in the past. Although all three approaches have yielded industrially viable processes, the latter two are the most widely used and can be regarded as complementary in many respects. Despite the progress in structural, computational and mechanistic studies, however, to date there is no universal recipe for the optimization of catalytic processes. Thus, a trial-and-error approach remains predominant in catalyst discovery and optimization. With the aim of complementing the well-established fields of homogeneous and enzymatic catalysis, organocatalysis and artificial metalloenzymes have enjoyed a recent revival. Artificial metalloenzymes, which are the focus of this book, result from comb- ing an active but unselective organometallic moiety with a macromolecular host. Kaiser and Whitesides suggested the possibility of creating artificial metallo- zymes as long ago as the late 1970s. However, there was a widespread belief that proteins and organometallic catalysts were incompatible with each other. This severely hampered research in this area at the interface between homogeneous and enzymatic catalysis. Since 2000, however, there has been a growing interest in the field of artificial metalloenzymes for enantioselective catalysis. The current state of the art and the potential for future development are p- sented in five well-balanced chapters. G. Roelfes, B. Feringa et al. summarize research relying on DNA as a macromolecular host for enantioselective catalysis.



Engineering The Genetic Code


Engineering The Genetic Code
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Author : Nediljko Budisa
language : en
Publisher: John Wiley & Sons
Release Date : 2006-05-12

Engineering The Genetic Code written by Nediljko Budisa and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-05-12 with Science categories.


The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research.