Factors Affecting Gene Targeting Efficiency In Murine Embryonic Stem Es Cells

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Factors Affecting Gene Targeting Efficiency In Murine Embryonic Stem Es Cells

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Author : Bryan Donald Parkes
language : en
Publisher:
Release Date : 1995

Factors Affecting Gene Targeting Efficiency In Murine Embryonic Stem Es Cells written by Bryan Donald Parkes and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Genetic engineering categories.

Gene Targeting And Embryonic Stem Cells

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Author : Jim Mcwhir
language : en
Publisher: Garland Science
Release Date : 2004-05-01

Gene Targeting And Embryonic Stem Cells written by Jim Mcwhir and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-01 with Science categories.

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.

Gene Targeting In Mouse Embryonic Stem Cells

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Author : Laura Hink Reid
language : en
Publisher:
Release Date : 1990

Gene Targeting In Mouse Embryonic Stem Cells written by Laura Hink Reid and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with categories.

Interface Oral Health Science 2016

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Author : Keiichi Sasaki
language : en
Publisher:
Release Date : 2020-10-08

Interface Oral Health Science 2016 written by Keiichi Sasaki and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-08 with Medical categories.

This volume broadens understanding of dentistry and promotes interdisciplinary research across a wide range of related fields, based on the symposium entitled "Innovative Research for Biosis-Abiosis Intelligent Interface 2016". It aims to create highly functional and autonomic intelligent interface by combining highly functional interface science with the technology of an evaluation and a control at the interface, with the various topics of biomaterials, innovation for oral science and application, regenerative oral science, and medical engineering. Since 2002, the Tohoku University Graduate School of Dentistry has hosted "Interface Oral Health Science" several times as the main theme of dental research in the twenty-first century, and this is the sixth proceedings of the symposiums following the ones in 2005, 2007, 2009, 2011, and 2014.This book benefits not only dental scientists but also other health scientists including medical physicians and pharmacologists, material scientists, engineers, and any scientist who is involved in variety of disciplines. This work was published by Saint Philip Street Press pursuant to a Creative Commons license permitting commercial use. All rights not granted by the work's license are retained by the author or authors.

Embryonic Stem Cells

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Author :
language : en
Publisher: Elsevier
Release Date : 2006-12-08

Embryonic Stem Cells written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-08 with Medical categories.

This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. * Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering * Presents the latest most innovative technologies * Addresses therapeutic relevance including FDA compliance and tissue engineering

Teratocarcinomas And Embryonic Stem Cells

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Author : Elizabeth J. Robertson
language : en
Publisher: Oxford University Press, USA
Release Date : 1987

Teratocarcinomas And Embryonic Stem Cells written by Elizabeth J. Robertson and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Cancer cells categories.

Heritable Human Genome Editing

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Author : The Royal Society
language : en
Publisher: National Academies Press
Release Date : 2021-01-16

Heritable Human Genome Editing written by The Royal Society and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-16 with Medical categories.

Heritable human genome editing - making changes to the genetic material of eggs, sperm, or any cells that lead to their development, including the cells of early embryos, and establishing a pregnancy - raises not only scientific and medical considerations but also a host of ethical, moral, and societal issues. Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably and without introducing undesired changes - criteria that have not yet been met, says Heritable Human Genome Editing. From an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.'s Royal Society, the report considers potential benefits, harms, and uncertainties associated with genome editing technologies and defines a translational pathway from rigorous preclinical research to initial clinical uses, should a country decide to permit such uses. The report specifies stringent preclinical and clinical requirements for establishing safety and efficacy, and for undertaking long-term monitoring of outcomes. Extensive national and international dialogue is needed before any country decides whether to permit clinical use of this technology, according to the report, which identifies essential elements of national and international scientific governance and oversight.

Gene Targeting

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Author : Alexandra L. Joyner
language : en
Publisher:
Release Date : 2000

Gene Targeting written by Alexandra L. Joyner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Science categories.

Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.

Human Embryonic Stem Cells

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Author : Jon Odorico
language : en
Publisher: Garland Science
Release Date : 2004-02-01

Human Embryonic Stem Cells written by Jon Odorico and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-02-01 with Science categories.

Since the first successful isolation and cultivation of human embryonic stem cells at the University of Wisconsin, Madison in 1998, there has been high levels of both interest and controversy in this area of research. This book provides a concise overview of an exciting field, covering the characteristics of both human embryonic stem cells and pluripotent stem cells from other human cell lineages. The following chapters describe state-of-the-art differentiation and characterization of specific ectoderm, mesoderm and endoderm-derived lineages from human embryonic stem cells, emphasizing how these can be used to study human developmental mechanisms. A further chapter discusses genetic manipulation of human ES cells. The concluding section covers therapeutic applications of human ES cells, as well as addressing the ethical and legal issues that this research have raised.

Sustainable Food Production

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Author : Paul Christou
language : en
Publisher: Springer
Release Date : 2012-12-05

Sustainable Food Production written by Paul Christou and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-05 with Technology & Engineering categories.

Gathering some 90 entries from the Encyclopedia of Sustainability Science and Technology, this book covers animal breeding and genetics for food, crop science and technology, ocean farming and sustainable aquaculture, transgenic livestock for food and more.