Gene Targeting
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Gene Targeting
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Author : Alexandra Joyner
language : en
Publisher: OUP Oxford
Release Date : 1999-12-09
Gene Targeting written by Alexandra Joyner and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-12-09 with Science categories.
Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
Gene Targeting
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Author : John M. Sedivy
language : en
Publisher: Oxford University Press, USA
Release Date : 1995
Gene Targeting written by John M. Sedivy and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Science categories.
Gene targeting offers new approaches to basic research and holds out the promise of eventual correction of genetic diseases. With simple, direct language and extensive illustrations, this advanced textbook offers a comprehensive review of homologous recombination and gene targeting techniques - the basic principles, methods, applicatiuons, and social implications.
Gene Targeting And Embryonic Stem Cells
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Author : Jim Mcwhir
language : en
Publisher: Garland Science
Release Date : 2004-05-01
Gene Targeting And Embryonic Stem Cells written by Jim Mcwhir and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-01 with Science categories.
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.
Gene Targeting Protocols
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Author : Eric B. Kmiec
language : en
Publisher: Humana
Release Date : 2013-08-14
Gene Targeting Protocols written by Eric B. Kmiec and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-14 with Medical categories.
The potential now exists in many experimental systems to transfer a cloned, modified gene back into the genome of the host organism. In the ideal situation, the cloned gene is returned to its homologous location in the genome and becomes inserted at the target locus. This process is a controlled means for the repair of DNA damage and ensures accurate chromosome disjunction during meiosis. The paradigm for thinking about the mechanism of this p- cess has emerged primarily from two sources: (1) The principles of reaction mechanics have come from detailed biochemical analyses of the RecA protein purified from Escherichia coli; and (2) the principles of information transfer have been derived from genetic studies carried out in bacteriophage and fungi. A compelling picture of the process of homologous pairing and DNA strand exchange has been influential in directing investigators interested in gene t- geting experiments. The ability to find and pair homologous DNA molecules enables ac- rate gene targeting and is the central phenomenon underlying genetic recombi- tion. Biochemically, the overall process can be thought of as a series of steps in a reaction pathway whereby DNA molecules are brought into homologous register, the four-stranded Holliday structure intermediate is formed, hete- duplex DNA is extended, and DNA strands are exchanged. Not much is known about the biochemical pathway leading to homologous recombination in euka- otes.
Gene Targeting
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Author : Manuel A. Vega
language : en
Publisher: CRC-Press
Release Date : 1994-10-25
Gene Targeting written by Manuel A. Vega and has been published by CRC-Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-10-25 with Science categories.
This book examines the revolutionary new technology of gene targeting, from its history to its potential application for the genetic treatment of human diseases. It begins with an introduction to the field and a description of the whole gene targeting spectrum. It continues with discussions in the following areas: The molecular mechanisms of the homologous recombination reactions lying behind gene targeting technology The application of gene targeting approaches to the problems of tripanosomatide biology and etiopathology The concept of gene targeting on plant genes and its impact on plant genetic engineering The current knowledge on mammalian development and physiology and its relationship to gene targeting technology The application of gene targeting technology, as well as other genetic techniques, to the production of animal models for human diseases The enzymatic complexes and components participating in natural bacterial DNA recombination and their potential application to gene targeting technology The potential application of gene targeting for the genetic treatment of human diseases
Gene Targeting
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Author : Alexandra L. Joyner
language : en
Publisher:
Release Date : 1993
Gene Targeting written by Alexandra L. Joyner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Gene targeting categories.
Gene Targeting
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Author : Alexandra L. Joyner
language : en
Publisher:
Release Date : 1997
Gene Targeting written by Alexandra L. Joyner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Laboratory Protocols For Conditional Gene Targeting
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Author : Raul M. Torres
language : en
Publisher: Oxford University Press on Demand
Release Date : 1997
Laboratory Protocols For Conditional Gene Targeting written by Raul M. Torres and has been published by Oxford University Press on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Science categories.
Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting The Institute of Genetics in Cologne and is intended for newcomers to the field as well as more experienced researchers. Topics covered include: the Cre/loxP recombination system and gene targeting in ES cells; gene targeting strategies; Cre/loxP recombination system; Gene targeting vectors consideration and use of loxP; nonselectable modifications and removal of selection marker genes; large deletions; gene replacement; chromosomal translocations; targeted integrations; conditional gene modification; LoxP-containing transgenes; conditional gene targeting; working with ES cells; ES cell transfection overview; embryonic feeder cells; ES cells handling and use; ES cell culture and transfection; ES clone picking; identification of homologous recombinants; freezing ES clones; thawing ES clones; genomic DNA preparation; cre-mediated neomycin deletion in ES cells; generation of mice preparation; mouse breeding for ES cell injection or aggregation; injection needles and holders; blastocyst preparation/injection; embryo aggregation; blastocyst transfer;
Gene Knockout Protocols
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Author : Martin J. Tymms
language : en
Publisher: Humana
Release Date : 2010-11-10
Gene Knockout Protocols written by Martin J. Tymms and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-10 with Medical categories.
As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources and the highly conserved gene conservation linkage organization. In just of ten years since the first gene-targeting experiments were p- formed in embryonic stem (ES) cells and mutations transmitted through the mouse germline, more than a thousand mouse strains have been created. These achievements have been made possible by pioneering work that showed that ES cells derived from preimplantation mouse embryos could be cultured for prolonged periods without differentiation in culture, and that homologous rec- bination between targeting constructs and endogenous DNA occurred at a f- quency sufficient for recombinants to be isolated. In the next few years the mouse genome will be systematically altered, and the techniques for achi- ing manipulations are constantly being streamlined and improved.
Gene Correction
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Author : Francesca Storici
language : en
Publisher: Humana Press
Release Date : 2014-02-21
Gene Correction written by Francesca Storici and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-21 with Medical categories.
Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.