Fine Structure Immunocytochemistry

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Fine Structure Immunocytochemistry

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Author : Gareth Griffiths
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Fine Structure Immunocytochemistry written by Gareth Griffiths and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Electron microscopy in the biological sciences can be divided into two disciplines. The first, concerned with high resolution detail of particles or periodic structures, is mostly based on sound theoretical principles of physics. The second, by far the larger discipline, is interested in the information obtainable from thin sections. The theoretical back ground to those groups of techniques for preparing and looking at thin sections is often inexact and "loose", for want of a better word. What should be chemistry is often closer to alchemy. This kind of electron microscopy is often enshrined with mystical recipes, handed down from generation to generation. Admittedly, many of the processes involved, such as those required to embed tissue in epoxy resins, involve multiple interconnected steps, which make it difficult to follow the details of anyone of these steps. If all these steps are shrouded in some mystery, however, can one really trust the final image that emerges on the EM screen? When we present the data in some semi quantitative form is there really no better way to do it than to categorize the parameters with ++, +/-, etc? What happens when one labels the sections with antibodies? Does the whole business necess arily need to be more of an "art" than a "science"? Upon reflecting on these problems in 1981, I had the impression that many of the multi-authored textbooks that existed then (and that have appeared since) tended to exacerbate or at least perpetuate this

Fine Structure Immunocytochemistry

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Author : Gareth Griffiths
language : en
Publisher: Springer
Release Date : 1993

Fine Structure Immunocytochemistry written by Gareth Griffiths and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Electron microscopic immunocytochemistry categories.

Fine Structure Immunocytochemistry

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Author : Gareth Griffiths
language : en
Publisher:
Release Date : 1993-01-26

Fine Structure Immunocytochemistry written by Gareth Griffiths and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-01-26 with categories.

Dna Viruses

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Author : Paul M. Lieberman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Dna Viruses written by Paul M. Lieberman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Medical categories.

A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

Neuroanatomical Tract Tracing

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Author : Laszlo Zaborszky
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-11-22

Neuroanatomical Tract Tracing written by Laszlo Zaborszky and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-11-22 with Medical categories.

The first two editions of this title had a tremendous impact in neuroscience. Between the Second edition in 1989 and today, there has been an explosion of information in the field, including advances in molecular techniques, such as genomics and proteomics, which have become increasing important in neuroscience. A renaissance in fluorescence has occurred, driven by the development of new probes, new microscopes, live imagers, and computer processing. The introduction of new markers has enormously stimulated the field, moving it from tissue culture to neurophysiology to functional MRI techniques.

Cytoplasmic Structures Advances In Research And Application 2012 Edition

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Author :
language : en
Publisher: ScholarlyEditions
Release Date : 2012-12-26

Cytoplasmic Structures Advances In Research And Application 2012 Edition written by and has been published by ScholarlyEditions this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-26 with Science categories.

Cytoplasmic Structures—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Cytoplasmic Structures. The editors have built Cytoplasmic Structures—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Cytoplasmic Structures in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Cytoplasmic Structures—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Immunohistochemistry And Immunocytochemistry

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Author : Simon Renshaw
language : en
Publisher: John Wiley & Sons
Release Date : 2017-02-15

Immunohistochemistry And Immunocytochemistry written by Simon Renshaw and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-02-15 with Science categories.

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.

Making And Using Antibodies

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Author : Gary C. Howard
language : en
Publisher: CRC Press
Release Date : 2006-12-13

Making And Using Antibodies written by Gary C. Howard and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-13 with Medical categories.

Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scienti

Mitochondrial Biogenesis And Genetics

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Author : Giuseppe Attardi
language : en
Publisher: Gulf Professional Publishing
Release Date : 1996

Mitochondrial Biogenesis And Genetics written by Giuseppe Attardi and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Aging categories.

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Structural and functional analysis of oxidative phosphorylation complexes * Import of proteins and RNA into mitochondria * Ion and metabolite transport systems in mitochondria * Biophysical methods for mitochondrial function analysis * Mitochondrial inheritance and turnover.

Biomedical Electron Microscopy

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Author : Arvid B. Maunsbach
language : en
Publisher: Elsevier
Release Date : 1998-11-03

Biomedical Electron Microscopy written by Arvid B. Maunsbach and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-11-03 with Science categories.

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. - Authored by the key leaders in the biological electron microscopy field - Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines - Introduces students on how to read and interpret electron micrographs