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Fixation In Histochemistry


Fixation In Histochemistry
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Fixation In Histochemistry


Fixation In Histochemistry
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Author : P. J. Stoward
language : en
Publisher: Springer
Release Date : 2013-11-11

Fixation In Histochemistry written by P. J. Stoward and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.


by by Professor Professor A. A. G. G. Everson Pearse Pearse The tide of of the Symposium, at at which the the papers which compromise this volume were presented, is ofthe utmost importance. It was not 'Fixation and Tissue Destruction' or 'Fixation 'Fixation and and Loss Loss ofTissue Components', Components', but but 'Fixation and and Tissue Tissue Preservation'. Historical Historical fixatives, fixatives, some some still still with with us us in the field oflight oflight microscopy microscopy after after over over 100 100 years, are are not not less less guilty guilty than than the the new new historical 'fixatives' of of ultrastructural ultrastructural practice practice some some ofwhich of which remove up up to to 60% 60% ofthe of the material material originally present in in the specimen and and convert the remainder into chemically inert products. There There must must be be few few histochemists histochemists who who do not not appreciate appreciate the great need need for for improve improve ments ments in in the the practice practice of of fixation, fixation, but but whence whence can can we we hopefully hopefully expect expect the arrival arrival of such such improvements? improvements ? Apart Apart from from out out ofthe ofthe blue, or revolutionary, revolutionary, advances advances such such as as the advent advent of of glutaralde hyde, there are perhaps three principal sources.



Fixation In Histochemistry


Fixation In Histochemistry
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Author : P. J. Stoward
language : en
Publisher:
Release Date : 1973-08

Fixation In Histochemistry written by P. J. Stoward and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973-08 with categories.




Histological Histochemical Methods


Histological Histochemical Methods
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Author : John Alan Kiernan
language : en
Publisher: Pergamon
Release Date : 1990

Histological Histochemical Methods written by John Alan Kiernan and has been published by Pergamon this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Medical categories.


Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc



Histological And Histochemical Methods Fifth Edition


Histological And Histochemical Methods Fifth Edition
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Author : John Kiernan
language : en
Publisher: Scion Publishing Ltd
Release Date : 2015-06-08

Histological And Histochemical Methods Fifth Edition written by John Kiernan and has been published by Scion Publishing Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-08 with Science categories.


This fifth edition of Histological and Histochemical Methods continues to provide a clear and consistent introduction to the techniques, description and analysis of the chemical and physical principles of fixation, tissue processing, staining, enzyme location, immunohistochemistry and other key procedures. The overall structure of the book remains unchanged, but the content has been heavily revised to update the techniques used in line with recent technological advances. Additionally, there are new sections on: Artefacts and troubleshooting Methods for microorganisms and fungi in sections Methods for various pigments and mineral deposits in tissues Methods for skeletal elements (bone, cartilage) in whole-mounts Histological and Histochemical Methods 5e is essential reading for students, lecturers, researchers and professionals using histological and histochemical techniques. From reviews: "Histological and Histochemical Methods is a tour de force wholly suited to the modern age of histology and Professor Kiernan has triumphed again. To cover so much ground clearly and concisely while including the justification of the underlying chemistry makes this book unique. There should not be a histology laboratory or an undergraduate library that does not own a copy." Biotechnic & Histochemistry 2016, 91(2): 145. "This book should be present on the bookshelves of every research or analysis laboratory where histology and histochemistry are routinely used, as an essential reference source of basic and practical information for scientists and technicians." European Journal of Histochemistry, 2016, vol. 60.



Handbook Of Histology Methods For Bone And Cartilage


Handbook Of Histology Methods For Bone And Cartilage
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Author : Yuehuei H. An
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-05-01

Handbook Of Histology Methods For Bone And Cartilage written by Yuehuei H. An and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-05-01 with Medical categories.


Histotechnology and histomorphometry are the major methodologies in bone and cartila- related research. Handbook of Histology Methods for Bone and Cartilage is an outgrowth of the editors’ own quest for information on bone and cartilage histology and histomorphometry. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. It is the first inclusive and organized reference book on histological and histomorphometrical techniques on bone and cartilage specimens. The topic has not previously been covered adequately by any existing books in the field. Handbook of Histology Methods for Bone and Cartilage has six major parts and is designed to be concise as well as inclusive, and more practical than theoretical. The text is simple and straightforward. Large numbers of tables, line drawings, and micro- or macro-photographs, are used to help readers better understand the content. Full bibliographies at the end of each chapter guide readers to more detailed information. A book of this length cannot discuss every method for bone and cartilage histology that has been used over the years, but it is hoped that major methods and their applications have been included.



Basic And Advanced Laboratory Techniques In Histopathology And Cytology


Basic And Advanced Laboratory Techniques In Histopathology And Cytology
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Author : Pranab Dey
language : en
Publisher: Springer Nature
Release Date : 2023-01-01

Basic And Advanced Laboratory Techniques In Histopathology And Cytology written by Pranab Dey and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-01 with Medical categories.


The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.



Histochemical Techniques


Histochemical Techniques
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Author : J. D. Bancroft
language : en
Publisher: Butterworth-Heinemann
Release Date : 2013-10-22

Histochemical Techniques written by J. D. Bancroft and has been published by Butterworth-Heinemann this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-22 with Science categories.


Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.



Histopathologic Technic And Practical Histochemistry


Histopathologic Technic And Practical Histochemistry
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Author : Ralph Dougall Lillie
language : en
Publisher: McGraw-Hill Companies
Release Date : 1976

Histopathologic Technic And Practical Histochemistry written by Ralph Dougall Lillie and has been published by McGraw-Hill Companies this book supported file pdf, txt, epub, kindle and other format this book has been release on 1976 with Histochemistry categories.


Micrscopy. Equipment. Fixation. Sctioning. General Staining ans mounting procedures. Stains and Fluorochromes. Nuclei, nucleic acids, general oversight methods. Chemical end groups. Cytopasmic ganules and organelles. Enzymes. Endogenous pigments. Metals, anions, exogenous pigments. Lipids. Polysaccharides, mucins. connective tissue fibers and membranes. Smear preparations, bacteria, protozoa, and other parasites. Glia and never cells and fibers. Hard tissues, decalcification. Various special procedures. Bufffers and buffers tables, normal acids and alkalies.



Histochemical And Immunohistochemical Techniques


Histochemical And Immunohistochemical Techniques
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Author : Peter H. Bach
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Histochemical And Immunohistochemical Techniques written by Peter H. Bach and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.


Toxicological and pharmacological effects arise when chemicals interact with biophysiological functions in discrete cell types. There is a continuing need to screen novel compounds for their potential therapeutic effects, and once these have been "discovered" to understand their molecular actions, as the basis of using such compounds safely and for rational drug design. Pharmacology now uses all of the sophisticated molecular research techniques that are available for the development of safer and more efficacious drugs. Histochemistry has been usefully applied to developing new drugs (and assessing chemical safley) and is potentially cost effective. The need to test novel substances for their potential adverse effects has raised many questions. Toxicological pathology has moved away from the cataloging of lesions towards understanding the basis of the events that underly cell injury, especially for those secondary consequences of chemical injury that lead to malignancy and chronic disease. The focal nature of toxicologic lesions de mands the use of microtechniques to provide data to help understand these questions. Histochemistry is under-utilized, but offers one of the key ap proaches necessary to address the problem of understanding interactions between a cell population and a chemical, the modulation of cellular biochem istryor the presence of a lesion in a test animal can be rationalised in terms of species differences that have no relevance to man as opposed to those that are of clinical significance or represent a warning of dire consequences to man.



Histochemistry


Histochemistry
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Author : Jinsong Zhou
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2017-08-21

Histochemistry written by Jinsong Zhou and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-08-21 with Science categories.


This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.