Flow Cytometry Protocols
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Flow Cytometry Protocols
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Author : Teresa S. Hawley
language : en
Publisher: Springer Nature
Release Date : 2024-03-25
Flow Cytometry Protocols written by Teresa S. Hawley and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-03-25 with Science categories.
This fifth edition volume expands on the previous editions by presenting readers with the latest developments and emerging methodologies in cytometry. The chapters in this book cover cytometry basics such as lasers for cytometry, metrics that can be used to evaluate spillover spreading, and the process of panel design and iterative optimization for spectral flow cytometry; novel methodologies such as image-enabled cell sorting, co-staining of fluorochrome-conjugated and oligonucleotide-conjugated antibodies, and screening for cell type selective probes; and a look at the achievements made in the clinical setting for both flow and mass cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Flow Cytometry Protocols, Fifth Edition is a valuable resource for researchers and scientists who are interested in continuing or expanding their knowledge of this developing field.
Imaging Flow Cytometry
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Author : Natasha S. Barteneva
language : en
Publisher: Humana
Release Date : 2015-11-23
Imaging Flow Cytometry written by Natasha S. Barteneva and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-11-23 with Medical categories.
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Current Protocols In Cytometry
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Author : J. Paul Robinson
language : en
Publisher:
Release Date : 2002
Current Protocols In Cytometry written by J. Paul Robinson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
Flow Cytometry Basics For The Non Expert
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Author : Christine Goetz
language : en
Publisher: Springer
Release Date : 2018-11-19
Flow Cytometry Basics For The Non Expert written by Christine Goetz and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-19 with Medical categories.
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.
Flow Cytometry
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Author : Alice Longobardi Givan
language : en
Publisher: John Wiley & Sons
Release Date : 2013-04-10
Flow Cytometry written by Alice Longobardi Givan and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-10 with Science categories.
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Practical Flow Cytometry
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Author : Howard M. Shapiro
language : en
Publisher: John Wiley & Sons
Release Date : 2005-02-25
Practical Flow Cytometry written by Howard M. Shapiro and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-25 with Science categories.
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Current Protocols In Immunology
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Author : John E. Coligan
language : en
Publisher: Current Protocols
Release Date : 1991
Current Protocols In Immunology written by John E. Coligan and has been published by Current Protocols this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Medical categories.
Current Protocols in Immunology is a three-volume looseleaf manual that provides comprehensive coverage of immunological methods from classic to the most cutting edge, including antibody detection and preparation, assays for functional activities of mouse and human cells involved in immune responses, assays for cytokines and their receptors, isolation and analysis of proteins and peptides, biochemistry of cell activation, molecular immunology, and animal models of autoimmune and inflammatory diseases. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Bimonthly updates, which are filed into the looseleaf, keep the set current with the latest developments in immunology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.
Flow Cytometry Applications In Cellular And Molecular Toxicology
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Author : AB Pant
language : en
Publisher: Springer Nature
Release Date : 2025-01-22
Flow Cytometry Applications In Cellular And Molecular Toxicology written by AB Pant and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-01-22 with Medical categories.
The book explores the role of flow cytometry in varied fields, from clinical diagnosis to toxicology. This comprehensive book offers insights into biomarkers, cellular analysis, and safety evaluations. Organized into fifteen chapters, this book explores flowcytometry's historical journey, scientific validation, and implementation in toxicity studies with case studies, technical and applied approaches, pictorial representations, informative tables, and simple language, It will be an invaluable resource for researchers, academia, biopharma industries, graduate and postgraduate students, Ph.D., and post-doctoral fellows working in the fields of toxicology/biosafety, and biomedical research.
Flow Cytometry Protocols
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Author : Teresa S. Hawley
language : en
Publisher:
Release Date : 2004
Flow Cytometry Protocols written by Teresa S. Hawley and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Flow cytometry categories.
In this thoroughly revised and updated edition of a widely used practical guide to flow cytometry, leading experimentalists describe in step-by-step detail an array of time-proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the fields of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high-throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T-cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures. The protocols presented follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Flow Cytometry Protocols, Second Edition offers both basic and clinical scientists an up-to-date collection of cutting-edge protocols for solving the widest variety of novel flow cytometry problems
Flow Cytometry
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Author : Stefanos Papandreou
language : en
Publisher: Nova Science Publishers
Release Date : 2013
Flow Cytometry written by Stefanos Papandreou and has been published by Nova Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with Flow cytometry categories.
Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characterisation of T regulatory and immune suppressor cells; multicolor panel design strategies in rare event analysis of dendritic cells and regulatory T cells; advanced characterisation of individual particle morphology light-scattering flow cytometry; and application of flow cytometry in environmental science.