Fluorescence Lifetime Imaging Ophthalmoscopy
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Fluorescence Lifetime Imaging Ophthalmoscopy
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Author : Martin Zinkernagel
language : en
Publisher: Springer
Release Date : 2019-07-29
Fluorescence Lifetime Imaging Ophthalmoscopy written by Martin Zinkernagel and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-29 with Medical categories.
This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natural course of disease, and to monitor the effects of possible therapies. This book fills the gap between available literature and gives state-of-the-art guidance on the principles of the FLIO technique, image acquisition, and data analysis. Written by a team of expert leaders within this field, this book will be relevant for scientists and clinicians with an interest in ophthalmoscopy.
Fluorescence Lifetime Imaging Ophthalmoscopy Flio From Bench To Bedside
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Author : Chantal-Simone Dysli
language : en
Publisher:
Release Date : 2017
Fluorescence Lifetime Imaging Ophthalmoscopy Flio From Bench To Bedside written by Chantal-Simone Dysli and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Two Photon Adaptive Optics Fluorescence Lifetime Imaging Ophthalmoscopy
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Author : James A.. Feeks
language : en
Publisher:
Release Date : 2018
Two Photon Adaptive Optics Fluorescence Lifetime Imaging Ophthalmoscopy written by James A.. Feeks and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
"There are many critical processes involved in keeping the retina functioning properly. Two of these, the visual cycle and the metabolism of the cell, are tied together by their conversion of important molecules from one form to another. In the visual cycle, 11-cis-retinal is regenerated so that it can combine with a rhodopsin molecule and initiate phototransduction. In cellular metabolism, the cell undergoes many steps to generate adenosine triphosphate, the energy unit of the cell. These mechanisms are critical in maintaining a functioning retina, however they have been difficult to directly interrogate in the living eye. A technique which can quantitatively measure these processes could allow researchers and clinicians to examine them in healthy subjects and how they change under conditions of disease. The goal of this work is to develop a technique which will allow us to investigate these measures of retinal function quantitatively and in a repeatable way. Advantageously, molecules which are converted during the visual cycle or cellular metabolism are accessible using adaptive optics aided two-photon fluorescence ophthalmoscopy. Furthermore, I develop a new technique, adaptive optics fluorescence lifetime ophthalmoscopy, which provides a robust and quantitative measure of a key property of retinal fluorescence. Initially, this method was deployed in a new two-photon adaptive optics ophthalmoscope designed for imaging mice. Exogenous fluorophores with known fluorescence lifetimes were used to validate the initial measurements, before using the new technique to establish baseline measurements for a sensor of cellular metabolism in the mouse eye. Following successful implementation in the mouse, the fluorescence lifetime method was translated to a system dedicated to imaging the macaque retina. By measuring the fluorescence lifetime of endogenous fluorescence originating in the photoreceptors, I found that rods and cones exhibit different fluorescence lifetimes. Further development of this technology may advance research in widespread areas including fluorophore identification in the retina, mechanisms of retinal metabolism, and as a clinical diagnostic."--Pages xiii-xiv.
Retinal Fluorescence Lifetime Imaging Ophthalmoscopy Measures Depend On The Severity Of Alzheimer S Disease
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Author :
language : en
Publisher:
Release Date : 2014
Retinal Fluorescence Lifetime Imaging Ophthalmoscopy Measures Depend On The Severity Of Alzheimer S Disease written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Monitoring Macular Pigment Changes In Macular Holes Using Fluorescence Lifetime Imaging Ophthalmoscopy
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Author :
language : en
Publisher:
Release Date : 2016
Monitoring Macular Pigment Changes In Macular Holes Using Fluorescence Lifetime Imaging Ophthalmoscopy written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Characterizing And Identifying The Fluorescence Lifetime Of The In Vivo Human Rpe Cellular Mosaic
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Author : Janet A H. Tang
language : en
Publisher:
Release Date : 2023
Characterizing And Identifying The Fluorescence Lifetime Of The In Vivo Human Rpe Cellular Mosaic written by Janet A H. Tang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.
"The retinal pigment epithelium (RPE) is a cell layer in the back of the eye essential for ocular health. With age, the RPE naturally accumulates autofluorescent material called lipofuscin, just one of many retinal fluorophores. Fluorescence is useful for diagnosing and tracking retinal disease. Beyond measuring intensity fluorescence lifetime imaging ophthalmoscopy (FLIO) varied with fluorophore composition and environmental factors that may provide critical insight into retinal mechanisms. Adaptive optics ophthalmoscopy can target specific retinal layers or individual cells. This thesis characterizes the in vivo human RPE layer using AOFLIO and investigates potential sources of change across the retina and with age. Firstly, it demonstrates the safety and repeatability of AOFLIO in human subjects with green-light excitation at 532 nm. By imaging at both the 532 nm spectral channel and mimicking the clinical device with two blue-light excitation channels - the long spectral channel (LSC) and short spectral channel (SSC) - I found that there was a high correlation between the LSC and 532 nm channel with a near-constant offset between the two lifetimes. That is likely because of a higher relative contribution of melanin to the 532 nm channel. To elucidate how AOFLIO results can be translated into the clinic, some subjects were also imaged with clinical FLIO. The AO LSC was well correlated with the clinical LSC, making the comparison simple between those channels. The AO 532 nm channel and the clinical LSC were not well correlated. The in vivo human signal in all 3 channels was compared to that from endogenous fluorophores in cuvettes. These results indicated that the main sources of fluorescence in the 532 nm channel are lipofuscin, melanin, and FAD where the increase of fluorescence lifetime with age can be attributed to the increase of melanolipofuscin which likely pulls the lifetime signal longer. Lastly, the cell-to-cell dynamics were investigated which found lifetime increases with age and eccentricity. The AO SSC changes were found to also be likely due to melanin changes across the retina. Future work will include applying AOFLIO to disease eyes to begin probing the dynamics of change with retinal degeneration."--Pages xiii-xiv.
Fluorescence Lifetime Imaging Ophthalmoscope And Supplement Of Agonistic Trkb Antibody In A Glaucoma Model
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Author : Aiwei Liu
language : en
Publisher:
Release Date : 2020
Fluorescence Lifetime Imaging Ophthalmoscope And Supplement Of Agonistic Trkb Antibody In A Glaucoma Model written by Aiwei Liu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with categories.
Two Photon Excited Fluorescence Adaptive Optics Ophthalmoscopy Of Retinal Function
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Author : Sarah Eileen Walters
language : en
Publisher:
Release Date : 2019
Two Photon Excited Fluorescence Adaptive Optics Ophthalmoscopy Of Retinal Function written by Sarah Eileen Walters and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
"The retina is the light-sensitive tissue at the back of the eye which carries out the first steps in vision. Specialized neural cells in the retina known as photoreceptors are responsible for detection of light and its transduction by initiating an electrical signal to the brain. Adaptive optics scanning light ophthalmoscopy (AOSLO), which dynamically corrects aberrations of the ocular media in the living eye and affords a lateral resolution of 2 ?m, has revolutionized our ability to visualize photoreceptors and many other microstructures in the retina. The implementation of two-photon excited fluorescence (TPEF) imaging in AOSLO has enabled not only complementary structural information throughout the retina, but an objective, non-invasive measure of visual function in photoreceptors by measuring TPEF kinetics from these cells. The aim of the present thesis is to further develop and apply TPEF ophthalmoscopy as a novel measure of in vivo cellular function in the retina. First, TPEF ophthalmoscopy was used in conjunction with other imaging modalities to evaluate the extent of photoreceptor dysfunction in a non-human primate model of retinal degeneration. TPEF ophthalmoscopy was essential in determining that photoreceptors were non-functional. Second, the sensitivity of TPEF kinetics to detect changes in photoreceptor function in conditions relevant to disease pathogenesis was investigated. Systemic hypoxia was employed in non-human primates as a model of physiological change, reducing oxygen supply to the retina, and TPEF kinetics were shown to be slowed as a consequence. Finally, the capabilities of TPEF ophthalmoscopy were expanded by implementing intrinsic fluorescence lifetime imaging. TPEF lifetime imaging was shown to distinguish retinal cell classes that are functionally disparate, and lifetimes were altered in regions of retinal damage. TPEF ophthalmoscopy has the potential to yield advances in understanding of both the basic physiology and pathology of the retina. If translated successfully into humans, TPEF ophthalmoscopy demonstrates promise as a valuable imaging modality that may, when used in conjunction with other clinical measures, identify early cellular dysfunction and longitudinally track pathological changes. Ultimately, it may assist in timely diagnosis, intervention, and development of treatments or vision restoration methods to combat blindness as a consequence of retinal disease."--Pages xiv-xv.
Theoretische Und Experimentelle Untersuchungen Zu Fluorescence Lifetime Imaging Ophthalmoscopy
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Author : Matthias Klemm
language : de
Publisher:
Release Date : 2017
Theoretische Und Experimentelle Untersuchungen Zu Fluorescence Lifetime Imaging Ophthalmoscopy written by Matthias Klemm and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Die vorliegende Arbeit befasst sich mit Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO), einem neuen Verfahren zur in vivo Messung der Autofluoreszenzabfälle endogener Fluorophore vom menschlichen Augenhintergrund. Die bisherigen Verfahren zur Approximation der Fluoreszenzlebensdauer aus den Autofluoreszenzabfällen stammen aus der Mikroskopie und sind daher nicht an die speziellen Bedingungen der in vivo Messungen am menschlichen Auge angepasst. Ziel der Arbeit war es, neue Verfahren der Messdatenverarbeitung zu entwickeln, welche die Fluoreszenzlebensdauerapproximation, insbesondere in FLIO-Daten, verbessern, sowie technische und physiologische Einflussfaktoren auf die FLIO-Methode zu untersuchen. Im Rahmen der Arbeit wurden daher neue Verfahren zur Behandlung von Artefakten durch eine inhomogene Fluoreszenzverteilung am Augenhintergrund sowie zur Behandlung von Artefakten durch Reflexionen im optischen Strahlengang des FLIO-Systems entwickelt. Die Modellierung der zeitaufgelösten Autofluoreszenzabfälle wurde erweitertet, um die Fluoreszenz des Augenhintergrunds als dreidimensionale Fläche zu betrachten, die Schichtstruktur des Auges abzubilden sowie die Fluoreszenz der natürlichen Augenlinse von einer separaten Messung zu berücksichtigen. Darüber hinaus wurden Verfahren entwickelt, welche durch Ausnutzung örtlicher Information Fehler in der Fluoreszenzlebensdauerapproximation reduzieren. Auf technischer Seite wurde die Wiederholbarkeit von FLIO anhand von Farbstoffen und Probanden untersucht, verschiedene FLIO-Systeme auf Vergleichbarkeit geprüft sowie die Eignung eines Weitwinkelobjektivs für FLIO-Messungen analysiert. Letztlich wurde der Einfluss der natürlichen Augenlinse und des Blutzuckerspiegels auf die Fluoreszenzlebensdauern des Augenhintergrunds in Patienten bzw. Probanden charakterisiert. Als ein Ergebnis der Arbeit entstand das umfangreiche Open-Source-Softwarepaket FLIMX, welches bestehende sowie die neu entwickelten Verfahren zur Fluoreszenzlebensdauerapproximation umsetzt. Außerdem bietet FLIMX Werkzeuge u.a. zur Simulation zeitaufgelöster Fluoreszenzdaten, zur Visualisierung von Fluoreszenzlebensdauern und anderer Parameter sowie zur statistischen Analyse einzelner FLIO-Messungen oder Probandengruppen. Anhand von Anwendungsbeispielen wurde die Wirksamkeit der neu entwickelten Verfahren demonstriert. Die Modellierung fluoreszierender Schichten wurde mittels aufwendiger Simulationen und experimenteller Messungen im Detail untersucht. Eine gute Wiederholbarkeit des FLIO-System konnte belegt werden. Beim Vergleich von vier FLIO-Geräten wies ein FLIO-Gerät moderate Abweichungen zu den übrigen FLIO-Geräten auf. Das Weitwinkelobjektiv liefert FLIO-Messungen mit einer guten Wiederholbarkeit, allerdings mit signifikanten Unterschieden zum Standardobjektiv. Der Blutzuckerspiegel hat keinen Einfluss auf die Fluoreszenzlebensdauern des Augenhintergrunds, die natürliche Augenlinse dagegen schon.
Einfluss Der Linsenfluoreszenz Auf Die Fluorescence Lifetime Imaging Ophthalmoscopy Flio Fundusbildgebung Und Strategien F R Dessen Kompensa
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Author : Jakob Lauritz Brauer
language : de
Publisher:
Release Date : 2021*
Einfluss Der Linsenfluoreszenz Auf Die Fluorescence Lifetime Imaging Ophthalmoscopy Flio Fundusbildgebung Und Strategien F R Dessen Kompensa written by Jakob Lauritz Brauer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021* with categories.