Fundamentals And Techniques Of Biophysics And Molecular Biology
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Fundamentals And Techniques Of Biophysics And Molecular Biology
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Author : Pranav Kumar
language : en
Publisher: Pathfinder Publication unit of PAPL
Release Date :
Fundamentals And Techniques Of Biophysics And Molecular Biology written by Pranav Kumar and has been published by Pathfinder Publication unit of PAPL this book supported file pdf, txt, epub, kindle and other format this book has been release on with Science categories.
Fundamentals and Techniques of Biophysics and Molecular Biology textbook has the primary goal to teach students about theoretical principles and applications of the key biophysical and molecular methods used in biochemistry and molecular biology. A substantial theoretical basis has been covered to understand key experimental techniques such as Chromatography, Electrophoresis, Spectroscopy, Mass spectrometry, Centrifugation, Microscopy, Flow cytometry, Chromatin immunoprecipitation, Immunotechniques, FRET and FRAP, Polymerase chain reaction, Phage display, Yeast two-hybrid assay, DNA sequencing, Biosensors, CRISPR/Cas systems so that students can make appropriate choices and efficient use of techniques. The most significant feature of this book is its clear, up-to-date and accurate explanations of mechanisms, rather than the mere description of facts and events. This book is published by Pathfinder Publication, New Delhi, India.
Introduction To Experimental Biophysics
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Author : Jay L. Nadeau
language : en
Publisher: CRC Press
Release Date : 2017-10-10
Introduction To Experimental Biophysics written by Jay L. Nadeau and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-10 with Medical categories.
Praise for the First Edition “essential reading for any physical scientist who is interested in performing biological research.” ―Contemporary Physics "an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions. Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.
Applied Biophysics
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Author : Thomas Andrew Waigh
language : en
Publisher: John Wiley & Sons
Release Date : 2007-09-11
Applied Biophysics written by Thomas Andrew Waigh and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-11 with Science categories.
This book presents the fundamentals of molecular biophysics, and highlights the connection between molecules and biological phenomena, making it an important text across a variety of science disciplines. The topics covered in the book include: Phase transitions that occur in biosystems (protein crystallisation, globule-coil transition etc) Liquid crystallinity as an example of the delicate range of partially ordered phases found with biological molecules How molecules move and propel themselves at the cellular level The general features of self-assembly with examples from proteins The phase behaviour of DNA The physical toolbox presented within this text will form a basis for students to enter into a wide range of pure and applied bioengineering fields in medical, food and pharmaceutical areas.
Biophysical Labeling Methods In Molecular Biology
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Author : Gertz I. Likhtenshtein
language : en
Publisher: Cambridge University Press
Release Date : 2005-08-22
Biophysical Labeling Methods In Molecular Biology written by Gertz I. Likhtenshtein and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-08-22 with Science categories.
This monograph describes the theoretical bases and experimental prerequisites for methods such as spin fluorescence, triplet, Mössbauer, photochromic and electron-density labeling, including the procedures used to obtain specifically modified proteins, enzymes, biomembranes, nucleic acids, and other biological molecules. The fundamentals of the physical theory behind each technique is explained and details are given of how to interpret the experimental data obtained. Special sections deal with critical reviews of recent data on the structure, molecular dynamics and conformational transitions of biological molecules. Each section concludes with a discussion of the results obtained from these techniques in connection with various problems of enzyme catalysis, electron transfer, molecular biophysics and molecular biology. The uses that labeling techniques can be put to for the investigation of whole cells and tissues are also discussed.
Introduction To Molecular Biology
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Author : S Bresler
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Introduction To Molecular Biology written by S Bresler and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Introduction to Molecular Biology focuses on the principles of polymer physics and chemistry and their applications to fundamental phenomena in biological sciences. It examines the structure, synthesis, and function of nucleic acids and proteins, as well as the physicochemical techniques necessary in determining the macromolecular structure, the kinetics and mechanism of enzyme action, the genetics of bacteria and their viruses, and the genetic code. It also considers the importance of precise quantitative analysis in biochemistry and biophysics, the architecture and function of biological macromolecules, and the unique mechanisms that regulate the cell's biological activity. Organized into five chapters, this book begins with an overview of proteins and their functional activity, from contractility and enzymatic catalysis to immunological activity, formation of selectively permeable membranes, and reversible binding and transport. It explains how such functions are related to molecular interactions and therefore fall within the purview of molecular biology. The book then proceeds with a discussion on the chemical structure of proteins and nucleic acids, the physicochemical techniques in measuring molecular size and shape, the mechanism of enzymatic reactions, the functions of DNA and RNA, and the mechanism of phase transition in polynucleotides. This book is intended for both biologists and non-biologists who want to be acquainted with the advances made in molecular biology, molecular genetics, and molecular biophysics during the 1950s and 1960s.
Progress In Biophysics And Molecular Biology
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Author :
language : en
Publisher:
Release Date : 1979
Progress In Biophysics And Molecular Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with Biochemistry categories.
Data Analysis In Biochemistry And Biophysics
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Author : Magar Mager
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Data Analysis In Biochemistry And Biophysics written by Magar Mager and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Data Analysis in Biochemistry and Biophysics describes the techniques how to derive the most amount of quantitative and statistical information from data gathered in enzyme kinetics, protein-ligand equilibria, optical rotatory dispersion, chemical relaxation methods. This book focuses on the determination and analysis of parameters in different models that are used in biochemistry, biophysics, and molecular biology. The Michaelis-Menten equation can explain the process to obtain the maximum amount of information by determining the parameters of the model. This text also explains the fundamentals present in hypothesis testing, and the equation that represents the statistical aspects of a linear model occurring frequently in this field of testing. This book also analyzes the ultraviolet spectra of nucleic acids, particularly, to establish the composition of melting regions of nucleic acids. The investigator can use the matrix rank analysis to determine the spectra to substantiate systems whose functions are not known. This text also explains flow techniques and relaxation methods associated with rapid reactions to determine transient kinetic parameters. This book is suitable for molecular biologists, biophysicists, physiologists, biochemists, bio- mathematicians, statisticians, computer programmers, and investigators involved in related sciences
Biophysical Techniques
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Author : Iain Campbell
language : en
Publisher: Oxford University Press
Release Date : 2012-02-16
Biophysical Techniques written by Iain Campbell and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-16 with Medical categories.
Biophysical Techniques explains in a readily-accessible way the basics of the various biophysical methods available so students can understand the principles behind the different methods used, and begin to appreciate which tools can be used to probe different biological questions, and the pros and cons of each.
Biophysical Tools For Biologists
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Author :
language : en
Publisher: Academic Press
Release Date : 2009-01-19
Biophysical Tools For Biologists written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-19 with Science categories.
Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method
Fundamental Concepts In Biophysics
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Author : Thomas Jue
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-04-20
Fundamental Concepts In Biophysics written by Thomas Jue and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-04-20 with Science categories.
In the first volume, Fundamental Concepts in Biophysics, the authors lay down a foundation for biophysics study. Rajiv Singh opens the book by pointing to the central importance of “Mathematical Methods in Biophysics”. William Fink follows with a discussion on “Quantum Mechanics Basic to Biophysical Methods”. Together, these two chapters establish some of the principles of mathematical physics underlying many biophysics techniques. Because computer modeling forms an intricate part of biophysics research, Subhadip Raychaudhuri and colleagues introduce the use of computer modeling in “Computational Modeling of Receptor–Ligand Binding and Cellular Signaling Processes”. Yin Yeh and coworkers bring to the reader’s attention the physical basis underlying the common use of fluorescence spectroscopy in biomedical research in their chapter “Fluorescence Spectroscopy”. Electrophysiologists have also applied biophysics techniques in the study of membrane proteins, and Tsung-Yu Chen et al. explore stochastic processes of ion transport in their “Electrophysiological Measurements of Membrane Proteins”. Michael Saxton takes up a key biophysics question about particle distribution and behavior in systems with spatial or temporal inhomogeneity in his chapter “Single–Particle Tracking”. Finally, in “NMR Measurement of Biomolecule Diffusion”, Thomas Jue explains how magnetic resonance techniques can map biomolecule diffusion in the cell to a theory of respiratory control. This book thus launches the Handbook of Modern Biophysics series and sets up for the reader some of the fundamental concepts underpinning the biophysics issues to be presented in future volumes.