Fundamentals Of Light Microscopy And Electronic Imaging


Fundamentals Of Light Microscopy And Electronic Imaging
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Fundamentals Of Light Microscopy And Electronic Imaging


Fundamentals Of Light Microscopy And Electronic Imaging
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Author : Douglas B. Murphy
language : en
Publisher: John Wiley & Sons
Release Date : 2012-11-05

Fundamentals Of Light Microscopy And Electronic Imaging written by Douglas B. Murphy and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-05 with Medical categories.


Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy



Video Microscopy


Video Microscopy
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Author : Shinya Inoue
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11

Video Microscopy written by Shinya Inoue and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Medical categories.


Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.



Introduction To Optical Microscopy


Introduction To Optical Microscopy
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Author : Jerome Mertz
language : en
Publisher: Cambridge University Press
Release Date : 2019-08

Introduction To Optical Microscopy written by Jerome Mertz and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08 with Medical categories.


Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.



Introduction To Light Microscopy


Introduction To Light Microscopy
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Author : Dee Lawlor
language : en
Publisher: Springer
Release Date : 2019-05-31

Introduction To Light Microscopy written by Dee Lawlor and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-31 with Science categories.


This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools.



Light And Electron Microscopy


Light And Electron Microscopy
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Author : Elizabeth M. Slayter
language : en
Publisher: Cambridge University Press
Release Date : 1992-10-30

Light And Electron Microscopy written by Elizabeth M. Slayter and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-10-30 with Science categories.


The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.



Fundamentals Of Light Microscopy


Fundamentals Of Light Microscopy
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Author : Michael Spencer
language : en
Publisher: CUP Archive
Release Date : 1982-07

Fundamentals Of Light Microscopy written by Michael Spencer and has been published by CUP Archive this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982-07 with Science categories.


Dr Spencer's account gives a concise but rigorous explanation of the principles underlying the various forms of light microscopy.



Video Microscopy


Video Microscopy
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Author : Shinya Inoué
language : en
Publisher: Springer
Release Date : 1997-08-31

Video Microscopy written by Shinya Inoué and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-08-31 with Medical categories.


The current edition of Video Microscopy has been totally revised to reflect the advances in the tools for electronic imaging, processing, recording, and analysis, as well as applications that are being made in video microscopy and related modes of electronic imaging. The less spiral organization of the revised edition reflects an audience expected to be more experi enced in video and computer image processing than in the earlier days when coupling of video equipment and computers to the light microscope was still considered to be a novelty. Nevertheless, we have emphasi2;ed the how-tos, as well as the fundamental principles, involved in imaging and processing ih the light microscope, the human visual system, video and related electronic imaging, and the digital image processor in the hope that the reader will develop enough understanding, not only to apply rationally what is available, but also to contribute actively to the development of this evolving field. In the text, the terms appearing in the Glossary are printed in bold type, generally, at first appearance. Italics are used for emphasis.



Fundamentals Of Fluorescence Microscopy


Fundamentals Of Fluorescence Microscopy
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Author : Partha Pratim Mondal
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-12

Fundamentals Of Fluorescence Microscopy written by Partha Pratim Mondal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-12 with Science categories.


This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.



Materials Characterization


Materials Characterization
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Author : Yang Leng
language : en
Publisher: John Wiley & Sons
Release Date : 2009-03-04

Materials Characterization written by Yang Leng and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-04 with Technology & Engineering categories.


This book covers state-of-the-art techniques commonly used in modern materials characterization. Two important aspects of characterization, materials structures and chemical analysis, are included. Widely used techniques, such as metallography (light microscopy), X-ray diffraction, transmission and scanning electron microscopy, are described. In addition, the book introduces advanced techniques, including scanning probe microscopy. The second half of the book accordingly presents techniques such as X-ray energy dispersive spectroscopy (commonly equipped in the scanning electron microscope), fluorescence X-ray spectroscopy, and popular surface analysis techniques (XPS and SIMS). Finally, vibrational spectroscopy (FTIR and Raman) and thermal analysis are also covered.



An Introduction To Microscopy


An Introduction To Microscopy
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Author : Suzanne Bell
language : en
Publisher: CRC Press
Release Date : 2009-10-21

An Introduction To Microscopy written by Suzanne Bell and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-21 with Law categories.


Microscopy, which has served as a fundamental scientific technique for centuries, remains an invaluable tool in chemistry, biology, healthcare, and forensics. Increasingly, it is being integrated into modern chemical instrumentation and is of value as a powerful analytical tool across many scientific disciplines. Designed to serve as a primary reso