Gene Targeting In Embryonic Stem Cells

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Gene Targeting And Embryonic Stem Cells

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Author : Jim Mcwhir
language : en
Publisher: Taylor & Francis
Release Date : 2004-03-01

Gene Targeting And Embryonic Stem Cells written by Jim Mcwhir and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-01 with Science categories.

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.

Transgenic Animal Technology

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Author : Carl A. Pinkert
language : en
Publisher: Newnes
Release Date : 2014-06-17

Transgenic Animal Technology written by Carl A. Pinkert and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-17 with Science categories.

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. - Contains a comprehensive collection of transgenic animal and gene transfer methods - Discusses background and introduction to techniques and animal systems - Teaches practical step-by-step protocols - Fully revised with updates to reflect state-of-the-art technology and associated changes to date

Gene Targeting And Embryonic Stem Cells

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Author : Jim Mcwhir
language : en
Publisher: Garland Science
Release Date : 2004-05-01

Gene Targeting And Embryonic Stem Cells written by Jim Mcwhir and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-01 with Science categories.

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.

Gene Targeting In Embryonic Stem Cells

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Author : Nicola Zink
language : de
Publisher:
Release Date : 2001

Gene Targeting In Embryonic Stem Cells written by Nicola Zink and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Embryonic stem cells categories.

Gene Knockout Protocols

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Author : Ralf Kühn
language : en
Publisher: Humana Press
Release Date : 2009-03-27

Gene Knockout Protocols written by Ralf Kühn and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-27 with Science categories.

Following the completion of the mouse and human genome sequences, a major challengeisthefunctionalcharacterizationofeverymammaliangeneandthedeciph- ing of their molecular interaction network. The mouse offers many advantages for the use of genetics to study human biology and disease, unmatched among other m- mals. Its development, body plan, physiology, behavior, and diseases have much in common, based on the fact that 99% of the human genes have a mouse ortholog. The investigation of gene function using mouse models is based on many years of tech- logical development. In the two decades since gene targeting in murine embryonic stem (ES) cells was first described by Mario Capecchi and colleagues, more than 3000 predesigned mouse mutants have been developed. To date, a variety of mouse mutagenesis techniques, either gene- or phenotype-driven, are used as systematic approaches. The availability of the genome sequence supports gene-driven approaches such as gene-trap and targeted mutagenesis in ES cells, allowing efficient and precise gene disruption. In combination with the use of site-specific DNA recombinases, in particular the Cre/loxP system, gene disruptioncan be directed to specific cell types in conditionalmousemutants. Furthermore,chemicalandtransposonmutagenesisofthe mouse genome enables us to perform phenotype-driven screens for the unbiased identification of phenotype–genotype correlations involved in models of human d- ease. Over the next several years, the mouse genome will be systematically altered, and the techniques for achieving predesigned manipulations will be constantly developed further and improved. The second edition of Gene Knockout Protocols brings together distinguished c- tributorswithextensiveexperienceinthegenetargetingandmousegeneticsfields.

Human Embryonic Stem Cells

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Author : Ann A. Kiessling
language : en
Publisher: Jones & Bartlett Learning
Release Date : 2003

Human Embryonic Stem Cells written by Ann A. Kiessling and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Medical categories.

The emerging field of human embryonic stem cell biomedicine crosses many disciplinary boundaries-cell biology, reproductive biology, embryology, molecular biology, endocrinology, immunology, fetal med

Handbook Of Stem Cells

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Author : Robert Paul Lanza
language : en
Publisher: Gulf Professional Publishing
Release Date : 2004

Handbook Of Stem Cells written by Robert Paul Lanza and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.

Accompanying CD-ROM (in v. 2) has image collections which can be saved in PowerPoint or HTML.

Embryonic Stem Cells

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Author : Kursad Turksen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Embryonic Stem Cells written by Kursad Turksen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

It is fair to say that embryonic stem (ES) cells have taken their place beside the human genome project as one of the most discussed biomedical issues of the day. It also seems certain that as this millennium unfolds we will see an increase in scientific and ethical debate about their potential utility in society. On the scientific front, it is clear that work on ES cells has already generated new possibilities and stimulated development of new strategies for increasing our und- standing of cell lineages and differentiation. It is not naïve to think that, within a decade or so, our overall understanding of stem cell biology will be as revolutionized as it was when the pioneering hemopoietic stem cell studies of Till and McCulloch in Toronto captured our imaginations in 1961. With it will come better methods for ES and lineage-specific stem cell identification, maintenance, and controlled fate selection. Clearly, ES cell models are already providing opportunities for the est- lishment of limitless sources of specific cell populations. In recognition of the gr- ing excitement and potential of ES cells as models for both the advancement of basic science and future clinical applications, I felt it timely to edit this collection of pro- cols (Embryonic Stem Cells) in which forefront investigators would provide detailed methods for use of ES cells to study various lineages and tissue types.

Gene Transfer To Animal Cells

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Author : Richard Twyman
language : en
Publisher: Taylor & Francis
Release Date : 2004-12-01

Gene Transfer To Animal Cells written by Richard Twyman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-12-01 with Science categories.

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.

Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition

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Author : Satish Kumar
language : en
Publisher:
Release Date : 1994

Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition written by Satish Kumar and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Genetic engineering categories.