Gene Targeting In Embryonic Stem Cells
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Gene Targeting And Embryonic Stem Cells
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Author : Jim Mcwhir
language : en
Publisher: Taylor & Francis
Release Date : 2004-03-01
Gene Targeting And Embryonic Stem Cells written by Jim Mcwhir and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-01 with Science categories.
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
Gene Targeting In Embryonic Stem Cells
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Author : Nicola Zink
language : de
Publisher:
Release Date : 2001
Gene Targeting In Embryonic Stem Cells written by Nicola Zink and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Embryonic stem cells categories.
Gene Targeting In Mouse Embryonic Stem Cells
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Author : Laura Hink Reid
language : en
Publisher:
Release Date : 1990
Gene Targeting In Mouse Embryonic Stem Cells written by Laura Hink Reid and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with categories.
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition
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Author : Satish Kumar
language : en
Publisher:
Release Date : 1994
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition written by Satish Kumar and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Genetic engineering categories.
Gene Targeting In Mammalian Embryonic Stem Cells
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Author : Kevin J. Spring
language : en
Publisher:
Release Date : 1996
Gene Targeting In Mammalian Embryonic Stem Cells written by Kevin J. Spring and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Embryonic stem cells categories.
Gene Knockout Protocols
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Author : Martin J. Tymms
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Gene Knockout Protocols written by Martin J. Tymms and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources and the highly conserved gene conservation linkage organization. In just of ten years since the first gene-targeting experiments were p- formed in embryonic stem (ES) cells and mutations transmitted through the mouse germline, more than a thousand mouse strains have been created. These achievements have been made possible by pioneering work that showed that ES cells derived from preimplantation mouse embryos could be cultured for prolonged periods without differentiation in culture, and that homologous rec- bination between targeting constructs and endogenous DNA occurred at a f- quency sufficient for recombinants to be isolated. In the next few years the mouse genome will be systematically altered, and the techniques for achi- ing manipulations are constantly being streamlined and improved.
Gene Targeting
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Author : Alexandra Joyner
language : en
Publisher: OUP Oxford
Release Date : 1999-12-09
Gene Targeting written by Alexandra Joyner and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-12-09 with Science categories.
Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition
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Author : Satish Kumar
language : en
Publisher:
Release Date : 1993
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition written by Satish Kumar and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Genetic engineering categories.
Gene Targeting In Murine Embryonic Stem Cells
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Author : Chuxia Deng
language : en
Publisher:
Release Date : 1992
Gene Targeting In Murine Embryonic Stem Cells written by Chuxia Deng and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Genetic engineering categories.
Gene Targeting And Insertional Mutagenesis In Embryonic Stem Cells
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Author : Helen Fiona Sutherland
language : en
Publisher:
Release Date : 1992
Gene Targeting And Insertional Mutagenesis In Embryonic Stem Cells written by Helen Fiona Sutherland and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Genes, Homeo box categories.