Genetic Diversity Segregation And Recombination In Common Bean Phaseolus Vulgaris L
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Genetic Diversity Segregation And Recombination In Common Bean Phaseolus Vulgaris L
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Author : Oscar Mario Paredes Cárcamo
language : en
Publisher:
Release Date : 1993
Genetic Diversity Segregation And Recombination In Common Bean Phaseolus Vulgaris L written by Oscar Mario Paredes Cárcamo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with categories.
Diversity And Functional Analysis Of The Common Bean Phaseolus Vulgaris L Determinacy Gene Pvtfl1y
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Author : Shelby Repinski
language : en
Publisher:
Release Date : 2012
Diversity And Functional Analysis Of The Common Bean Phaseolus Vulgaris L Determinacy Gene Pvtfl1y written by Shelby Repinski and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Common bean (Phaseolus vulgaris) plants exhibit two distinct stem termination growth habit types: indeterminate and determinate. Plants with indeterminate growth habit have a terminal meristem that remains vegetative throughout the production of lateral vegetative and reproductive growth. Contrarily, in determinate plants, the terminal shoot meristem switches from a vegetative to reproductive state, resulting in a terminal inflorescence. While only indeterminate growth is observed in wild-type common bean populations, both growth habits can be observed in domesticated varieties. Determinate growth habit has been artificially selected, in combination with photoperiod insensitivity, to obtain varieties with a shortened and synchronized flowering period and earlier maturation, allowing mechanized harvest. In the early 1990s, researchers in Arabidopsis thaliana identified a gene, Terminal Flowering 1 (TFL1), as a locus controlling inflorescence meristem development. Three homologs of TFL1 were identified in common bean: Phaseolus vulgaris Terminal Flowering 1 x, y, and z (PvTFL1x, PvTFL1y, and PvTFL1z). PvTFL1y was the only candidate gene to contain natural genetic variation potentially correlated with determinacy and also co-segregate with the phenotypic locus for determinate growth habit (fin) on chromosome 01. In chapter one, our goal was to verify the role of PvTFL1y in determining growth habit. Using quantitative PCR, we found that two distinct mutant PvTFL1y haplotypes associated with determinacy caused mRNA abundance to decrease 20-133 fold; consistent with the recessive nature of fin. Furthermore, we were able to rescue mutant determinate (tfl1-1) Arabidopsis plants by transformation with the wild-type PvTFL1y gene. This work validates that the candidate gene, PvTFL1y, is the functional homolog of TFL1 and is the molecular locus underlying the fin phenotypic locus. In chapter two, we use the pervasive synteny among legumes species in a comparative genomics approach to identify growth habit homologs of PvTFL1y in 16 different Phaseolus species; two of which include accessions with a determinate growth habit: P. coccineus and P. lunatus. Neighbor-joining analyses of amplified homologs verified previous genetic relationships in the Phaseolus species. Additionally, a 2bp insertion/deletion (indel) in the P. lunatus homolog, PlTFL1, was found to segregate with growth habit. We created a Cleaved Amplified Polymorphic sequence (CAPs) marker for this indel that can be utilized in future co-segregation studies. No mutation could yet be found that could be causal to determinacy in P. coccineus. Several unique mutant alleles for determinate growth habit have been identified at the PvTFL1y locus and it is of interest whether they confer differences in flowering and plant architecture. In chapter three, we analyzed dissimilarities between four mutant PvTFL1y haplotypes by transferring them, using marker-assisted backcrossing, into a common genetic background. Most notable, one backcross population containing the most prevalent mutant haplotype, a 4.1kb retrotransposon, was found to begin and finish flowering significantly earlier than the recurrent parent and all other backcross populations. It is likely that determinate growth habit in association with earliness may have caused this haplotype to become the most agronomically desirable during or after domestication. More generations of backcrossing followed by recurrent parent background genetic selection will elucidate if identified trait associations are caused by pleiotropy or linkage. Common bean was domesticated independently in Mesoamerica and in the Andes. Within the Mesoamerican center, the domestication area has been narrowed to the Lerma-Santiago Basin of Mexico, while the Andean center still remains quite large. During Andean domestication, determinate growth habit was likely selected for as it led to a more compact growth habit and earliness. By analyzing shared haplotypes between wild and domesticated common bean, around the domestication locus PvTFL1y, it may be possible to narrow the Andean center of domestication. In chapter four we analyzed an 800 kb region around PvTFL1y, in 96 wild and domesticated common bean accessions. Preliminary findings have identified three unique haplotypes for growth habit, indicating that selection for determinacy, in the Andean gene pool, may have occurred several times since domestication. Two determinacy haplotypes appear most closely related to wild samples from Peru while the other clustered with Argentinian accessions. Complications in the analysis arose from high levels of admixture amongst wild accessions and inconsistent sequencing coverage across accessions sampled. Future studies should include important samples in replicate and reduce pooling to achieve maximum coverage across all accessions.
Phaseolin And Isozyme Diversity In Wild Phaseolus Vulgaris L
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Author : Rosalie L. Koenig
language : en
Publisher:
Release Date : 1988
Phaseolin And Isozyme Diversity In Wild Phaseolus Vulgaris L written by Rosalie L. Koenig and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.
Proccedings Of The Second International Scientific Meeting Phaseolus Beans Advanced Biotechnology Research Network Barn
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Author :
language : en
Publisher: CIAT
Release Date :
Proccedings Of The Second International Scientific Meeting Phaseolus Beans Advanced Biotechnology Research Network Barn written by and has been published by CIAT this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Population Genomics Crop Plants
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Author : Om P. Rajora
language : en
Publisher: Springer Nature
Release Date :
Population Genomics Crop Plants written by Om P. Rajora and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Genomics Of Plant Genetic Resources
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Author : Roberto Tuberosa
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-08
Genomics Of Plant Genetic Resources written by Roberto Tuberosa and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-08 with Science categories.
Our lives and well being intimately depend on the exploitation of the plant genetic resources available to our breeding programs. Therefore, more extensive exploration and effective exploitation of plant genetic resources are essential prerequisites for the release of improved cultivars. Accordingly, the remarkable progress in genomics approaches and more recently in sequencing and bioinformatics offers unprecedented opportunities for mining germplasm collections, mapping and cloning loci of interest, identifying novel alleles and deploying them for breeding purposes. This book collects 48 highly interdisciplinary articles describing how genomics improves our capacity to characterize and harness natural and artificially induced variation in order to boost crop productivity and provide consumers with high-quality food. This book will be an invaluable reference for all those interested in managing, mining and harnessing the genetic richness of plant genetic resources.
The Common Bean Genome
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Author : Marcelino Pérez de la Vega
language : en
Publisher: Springer
Release Date : 2017-12-20
The Common Bean Genome written by Marcelino Pérez de la Vega and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-20 with Science categories.
This book provides insights into the genetics and the latest advances in genomics research on the common bean, offering a timely overview of topics that are pertinent for future developments in legume genomics. The common bean (Phaseolus vulgaris L.) is the most important grain legume crop for food consumption worldwide, as well as a model for legume research, and the availability of the genome sequence has completely changed the paradigm of the ongoing research on the species. Key topics covered include the numerous genetic and genomic resources, available tools, the identified genes and quantitative trait locus (QTL) identified, and there is a particular emphasis on domestication. It is a valuable resource for students and researchers interested in the genetics and genomics of the common bean and legumes in general.
Crop Science
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Author :
language : en
Publisher:
Release Date : 2010
Crop Science written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Crop science categories.
Dissertation Abstracts International
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Author :
language : en
Publisher:
Release Date : 2004
Dissertation Abstracts International written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Dissertations, Academic categories.
Genetics Abstracts
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Author :
language : en
Publisher:
Release Date : 2001
Genetics Abstracts written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Genetics categories.