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The Common Bean Genome


The Common Bean Genome
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The Common Bean Genome


The Common Bean Genome
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Author : Marcelino Pérez de la Vega
language : en
Publisher: Springer
Release Date : 2017-12-20

The Common Bean Genome written by Marcelino Pérez de la Vega and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-20 with Science categories.


This book provides insights into the genetics and the latest advances in genomics research on the common bean, offering a timely overview of topics that are pertinent for future developments in legume genomics. The common bean (Phaseolus vulgaris L.) is the most important grain legume crop for food consumption worldwide, as well as a model for legume research, and the availability of the genome sequence has completely changed the paradigm of the ongoing research on the species. Key topics covered include the numerous genetic and genomic resources, available tools, the identified genes and quantitative trait locus (QTL) identified, and there is a particular emphasis on domestication. It is a valuable resource for students and researchers interested in the genetics and genomics of the common bean and legumes in general.



Molecular Mapping And Characterization Of Phenylpropanoid Pathway Genes In Common Bean Phaseolus Vulgaris L


Molecular Mapping And Characterization Of Phenylpropanoid Pathway Genes In Common Bean Phaseolus Vulgaris L
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Author : Zeinab Yadegari
language : en
Publisher:
Release Date : 2013

Molecular Mapping And Characterization Of Phenylpropanoid Pathway Genes In Common Bean Phaseolus Vulgaris L written by Zeinab Yadegari and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.




Genetic Resources Of Phaseolus Beans


Genetic Resources Of Phaseolus Beans
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Author : Paul Gepts
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Genetic Resources Of Phaseolus Beans written by Paul Gepts and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


The period following the second world war has witnessed an expanding commitment to incr~ased food production in tropical countries. Public and private initiatives at the national and international levels have led to the creation of programs geared specifically towards the improvement of food crops in tropical conditions. Examples of this increased commitment are the network of international agricultural research centers and numerous bilateral aid projects. As a consequence, crop improvement has become a truly worldwide endeavor, relying on an international network of institutions and collaborators. This holds also for Phaseolus beans. Following the discovery of the Americas, Phaseolus beans became distributed on all six continents. Yet, until not so long ago, most of the research on Phaseolus improvement took place in developed countries. In recognition of the nutritional importance of Phaseolus beans in developing countries, this has changed considerably in the last years, principally perhaps through the activities of the Centro Internacional de Agricultura Tropical (CIAT) and the International Board for Plant Genetic Resources (IBPGR). Consequently, the scope of the research on Phaseolus has broadened considerably and the number of Phaseolus researchers is larger than ever before.



Genetic And Genomic Resources Of Grain Legume Improvement


Genetic And Genomic Resources Of Grain Legume Improvement
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Author : Lucia Lioi
language : en
Publisher: Elsevier Inc. Chapters
Release Date : 2013-07-18

Genetic And Genomic Resources Of Grain Legume Improvement written by Lucia Lioi and has been published by Elsevier Inc. Chapters this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-07-18 with Science categories.


In this chapter, we lead the reader through several topics related to common bean germplasm, including crop dissemination across the Old World, landraces developed by farmers, characterization and safeguard of germplasm. These topics are attracting a great deal of attention, especially in recent years, when the international community has become increasingly aware of the relevance of safeguarding plant genetic resources. Early studies were mainly aimed at performing exploratory analyses of agro-morphological traits, with the goal of improving yield and pest resistance. Moreover, phaseolin variation was studied to check the distribution of the two common bean gene pools. The recent development of DNA-based markers has allowed significant improvement in knowledge on genetic variation within the European germplasm, as well as the capacity to trace its divergence from the American germplasm. The different strategies applied to safeguard the European germplasm are described together with their drawbacks.



Characterization Of A Putative Yield Related Gene In Common Bean Phaseolus Vulgaris L


Characterization Of A Putative Yield Related Gene In Common Bean Phaseolus Vulgaris L
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Author : YANZHOU. QI
language : en
Publisher:
Release Date : 2015

Characterization Of A Putative Yield Related Gene In Common Bean Phaseolus Vulgaris L written by YANZHOU. QI and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.




Toward Genomics Based Breeding In Phaseolus Vulgaris And Quantitative Trait Locus Mapping Of Angular Leaf Spot Resistance


Toward Genomics Based Breeding In Phaseolus Vulgaris And Quantitative Trait Locus Mapping Of Angular Leaf Spot Resistance
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Author : Tamara Iva Miller
language : en
Publisher:
Release Date : 2019

Toward Genomics Based Breeding In Phaseolus Vulgaris And Quantitative Trait Locus Mapping Of Angular Leaf Spot Resistance written by Tamara Iva Miller and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.


The common bean (Phaseolus vulgaris L.) is consumed by millions of people worldwide and is a staple source of protein, starch and micronutrients. Common bean production across the world is affected by abiotic and biotic stresses that limit the growth and yield of this important crop. Efforts to breed improved common bean for dissemination to farmers and consumers in East Africa is underway in several breeding programs worldwide. Improvement on agronomic and consumer traits such as disease resistance can be greatly aided by the application of next generation sequencing technologies. With the decreasing cost of DNA sequencing, genomic re-sequencing of diverse common bean accessions facilitates marker- assisted breeding that can be used to speed the creation of new common bean cultivars. Marker-assisted selection (MAS) is an important aspect of modern bean breeding that seeks to utilize genetic markers to select individuals with improved agronomic and consumer traits. For example, breeders in the African Bean Consortium seek to introgress known genetic loci conferring resistance to multiple diseases into bean genetic backgrounds with preferred seed and agronomic characteristics. However, the usefulness of markers is dependent on whether they are polymorphic in the specific parents of the breeding program. Often genetic markers identified in a specific plant population are not useful for marker assisted selection among a different set of bean parents, which necessitates identification of novel markers linked to the genes of interest that are polymorphic among breeding parents. One disease that greatly affects common bean production in humid tropical and sub-tropical growing regions is Angular Leaf Spot (ALS; caused by the foliar fungus Pseudocercospora griseola Sacc.). Marker assisted breeding is being used in multiple different bean breeding programs to improve the resistance of adapted cultivars to ALS. The ALS resistance locus, Phg-2, is an important resistance locus used to improve plant resistance to Angular Leaf Spot in South America and Pan Africa, however in the case of the African Bean Consortium breeding programs in East Africa, certain bean parents used for breeding were monomorphic for the original marker used to perform marker assisted selection of Phg-2. In order to facilitate marker assisted selection of Phg-2 in specific breeding parents used in the Uganda bean improvement program, an alternative, co-dominant, marker linked to the Phg-2 ALS resistance locus was developed (Chapter 1). A new marker, g796, was identified which is polymorphic among the breeding parents; its co-segregation was confirmed in a segregating F2 population derived from the cross between French bean variety Amy and the ALS resistance donor, Mexico 54. This work was conducted in collaboration with Stephen Kimno and Esther Arunga at Embu University, Kenya, as well as other members of the African Bean Consortium bean breeding programs in Tanzania, Uganda, and Ethiopia. The application of DNA sequencing to marker-assisted breeding and crop improvement is rapidly becoming common in the development of improved bean varieties. A nearly complete reference genome and transcriptome for Phaseolus vulgaris was released in 2014 and newly resequenced genomes of diverse bean accessions are being developed for the purpose of marker assisted breeding. In Chapter 2, whole-genome resequencing of 29 bean accessions, including accessions commonly used as breeding parents, was carried out in collaboration with the Ratz lab at the International Center for Tropical Agriculture (CIAT, Colombia). Genetic diversity analysis was performed in order to access the evolutionary relationships between the sequenced bean genomes. Data generated by this work was made available to the larger bean research community and will be used by breeders and geneticists to perform marker-assisted selection and genetic analysis in the future. Angular leaf spot (ALS) occurs throughout Eastern and Southern Africa (as well as other parts of the world) and can cause yield losses up to 80% in environments that favor the disease. ALS is caused by the fungal pathogen, Pseudocercospora griseola, a highly diverse pathogen with many different races that infect diverse types of bean hosts. Growing crop cultivars with genetic resistance to the disease is one of the most effective measures for farmers to reduce crop losses due to ALS. The landrace Mexico 54 is used as a donor for ALS resistance in East Africa and marker-assisted selection of the Phg-2 ALS resistance locus from Mexico 54 is underway in multiple breeding programs in order to increase the resistance of adapted bean germplasm in East Africa and Brazil. Previous allelism tests between different ALS resistance donors suggested additional resistance loci exist in Mexico 54 besides the Phg-2 locus and were named Phg-5 and Phg-6. The genomic locations of the proposed Phg-5 and Phg-6 resistance genes in Mexico 54 have never been investigated, however, the existence of multiple resistance loci in Mexico 54 is likely the cause of its high level of resistance to ALS on multiple continents. In Chapter 3, a biparental mapping population consisting of 167 F8 recombinant inbred lines (RIL) was derived from a cross between Kablanketi, a preferred bean market type in Tanzania, and Mexico 54 in order to map additional quantitative trait loci that confer resistance to ALS in Mexico 54. The identification of novel ALS resistance loci will aid breeders to develop resistant cultivars as well as provide a greater understanding of the genetic diversity that influences resistance to ALS.



Diversity And Functional Analysis Of The Common Bean Phaseolus Vulgaris L Determinacy Gene Pvtfl1y


Diversity And Functional Analysis Of The Common Bean Phaseolus Vulgaris L Determinacy Gene Pvtfl1y
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Author : Shelby Repinski
language : en
Publisher:
Release Date : 2012

Diversity And Functional Analysis Of The Common Bean Phaseolus Vulgaris L Determinacy Gene Pvtfl1y written by Shelby Repinski and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.


Common bean (Phaseolus vulgaris) plants exhibit two distinct stem termination growth habit types: indeterminate and determinate. Plants with indeterminate growth habit have a terminal meristem that remains vegetative throughout the production of lateral vegetative and reproductive growth. Contrarily, in determinate plants, the terminal shoot meristem switches from a vegetative to reproductive state, resulting in a terminal inflorescence. While only indeterminate growth is observed in wild-type common bean populations, both growth habits can be observed in domesticated varieties. Determinate growth habit has been artificially selected, in combination with photoperiod insensitivity, to obtain varieties with a shortened and synchronized flowering period and earlier maturation, allowing mechanized harvest. In the early 1990s, researchers in Arabidopsis thaliana identified a gene, Terminal Flowering 1 (TFL1), as a locus controlling inflorescence meristem development. Three homologs of TFL1 were identified in common bean: Phaseolus vulgaris Terminal Flowering 1 x, y, and z (PvTFL1x, PvTFL1y, and PvTFL1z). PvTFL1y was the only candidate gene to contain natural genetic variation potentially correlated with determinacy and also co-segregate with the phenotypic locus for determinate growth habit (fin) on chromosome 01. In chapter one, our goal was to verify the role of PvTFL1y in determining growth habit. Using quantitative PCR, we found that two distinct mutant PvTFL1y haplotypes associated with determinacy caused mRNA abundance to decrease 20-133 fold; consistent with the recessive nature of fin. Furthermore, we were able to rescue mutant determinate (tfl1-1) Arabidopsis plants by transformation with the wild-type PvTFL1y gene. This work validates that the candidate gene, PvTFL1y, is the functional homolog of TFL1 and is the molecular locus underlying the fin phenotypic locus. In chapter two, we use the pervasive synteny among legumes species in a comparative genomics approach to identify growth habit homologs of PvTFL1y in 16 different Phaseolus species; two of which include accessions with a determinate growth habit: P. coccineus and P. lunatus. Neighbor-joining analyses of amplified homologs verified previous genetic relationships in the Phaseolus species. Additionally, a 2bp insertion/deletion (indel) in the P. lunatus homolog, PlTFL1, was found to segregate with growth habit. We created a Cleaved Amplified Polymorphic sequence (CAPs) marker for this indel that can be utilized in future co-segregation studies. No mutation could yet be found that could be causal to determinacy in P. coccineus. Several unique mutant alleles for determinate growth habit have been identified at the PvTFL1y locus and it is of interest whether they confer differences in flowering and plant architecture. In chapter three, we analyzed dissimilarities between four mutant PvTFL1y haplotypes by transferring them, using marker-assisted backcrossing, into a common genetic background. Most notable, one backcross population containing the most prevalent mutant haplotype, a 4.1kb retrotransposon, was found to begin and finish flowering significantly earlier than the recurrent parent and all other backcross populations. It is likely that determinate growth habit in association with earliness may have caused this haplotype to become the most agronomically desirable during or after domestication. More generations of backcrossing followed by recurrent parent background genetic selection will elucidate if identified trait associations are caused by pleiotropy or linkage. Common bean was domesticated independently in Mesoamerica and in the Andes. Within the Mesoamerican center, the domestication area has been narrowed to the Lerma-Santiago Basin of Mexico, while the Andean center still remains quite large. During Andean domestication, determinate growth habit was likely selected for as it led to a more compact growth habit and earliness. By analyzing shared haplotypes between wild and domesticated common bean, around the domestication locus PvTFL1y, it may be possible to narrow the Andean center of domestication. In chapter four we analyzed an 800 kb region around PvTFL1y, in 96 wild and domesticated common bean accessions. Preliminary findings have identified three unique haplotypes for growth habit, indicating that selection for determinacy, in the Andean gene pool, may have occurred several times since domestication. Two determinacy haplotypes appear most closely related to wild samples from Peru while the other clustered with Argentinian accessions. Complications in the analysis arose from high levels of admixture amongst wild accessions and inconsistent sequencing coverage across accessions sampled. Future studies should include important samples in replicate and reduce pooling to achieve maximum coverage across all accessions.



Common Bean Improvement In The Twenty First Century


Common Bean Improvement In The Twenty First Century
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Author : S.P. Singh
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-09

Common Bean Improvement In The Twenty First Century written by S.P. Singh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-09 with Science categories.


The common bean (Phaseolus vulgaris L. ) is the most important pulse crop in the world. It is an important source of calories, proteins, dietary fibers, minerals, and vitamins for millions of people in both developing and developed countries worldwide. It complements cereals and other carbohydrate-rich foods in providing near-perfect nutrition to people of all ages. Moreover, a regular intake ofbeans helps lower cholesterol and cancer risks. Despite the fact that per capita consumption of common bean in some developed countries (e. g. , the U. S. A. ) has been increasing over the last several years, in general, the average global per capita consumption is declining because production is unable to keep up with the population growth. Moreover, increasing demand for pesticide-free food products, concern for natural resources conservation, and the need to reduce production costs offer daunting challenges to the twenty-first century policy makers, bean growers, and researchers alike. High yielding, high quality bean cultivars that require less water, fertilizers, pesticides, and manual labor combined with integrated management of abiotic and biotic stresses will have to be developed. Eminent bean researchers were invited to contemplate these issues, prepare a state-of-the-art account on most relevant topics, and offer their insight into research directions into the twenty-first century. Four excellent books have been published covering various aspects ofthe common bean since 1980. These books are: I) Bean Production Problems nd in the Tropics (l SI ed. 1980, 2 ed. 1989), H. F. Schwartz & M. A.



Genetic Diversity Segregation And Recombination In Common Bean Phaseolus Vulgaris L


Genetic Diversity Segregation And Recombination In Common Bean Phaseolus Vulgaris L
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Author : Oscar Mario Paredes Cárcamo
language : en
Publisher:
Release Date : 1993

Genetic Diversity Segregation And Recombination In Common Bean Phaseolus Vulgaris L written by Oscar Mario Paredes Cárcamo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with categories.




Dna Based Markers In Plants


Dna Based Markers In Plants
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Author : R.L. Phillips
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-14

Dna Based Markers In Plants written by R.L. Phillips and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-14 with Science categories.


With the new techniques described in this volume, a new gene can be placed on the linkage map within only a few days. Leading researchers have updated the earlier edition to include the latest versions of DNA-based marker maps for a variety of important crops.