Guide To Molecular Cloning Techniques
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Guide To Molecular Cloning Techniques
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Author :
language : en
Publisher: Academic Press
Release Date : 1987-09-28
Guide To Molecular Cloning Techniques written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-09-28 with Science categories.
This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.
A Practical Guide To Molecular Cloning
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Author : Bernard V. Perbal
language : en
Publisher: Wiley-Interscience
Release Date : 1984
A Practical Guide To Molecular Cloning written by Bernard V. Perbal and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Genetic engineering categories.
Presents techniques tested at the Curie Institute and other leading labs and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. Offers detailed explanation of protocols, allowing the isolation, cloning, and expression of genes from living species. Presents up-to-date techniques on sequencing, in vitro expression of cloned gene, and use of computers for study of nucleic acids, and is the only book that shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes. Contains 235 figures and 80 tables.
A Practical Guide To Molecular Cloning
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Author : Bernard Perbal
language : en
Publisher: Wiley-Liss
Release Date : 2008-10-01
A Practical Guide To Molecular Cloning written by Bernard Perbal and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-10-01 with Science categories.
This third edition of A Practical Guide to Molecular Cloning provides complete updates to all of the protocols, and incorporates much new material to cover a broader range of biomolecular techniques. It covers the key information required for students to perform cloning of any DNA fragment and eventually study its expression products. It also serves as an excellent comprehensive guide for any researcher using molecular techniques. Protocols in this established manual have been used successfully to characterize cellular localization, nature, and function of gene products in both prokaryotes and eukaryotes. This book is the most accessible laboratory manual for those who want to understand why techniques work and how best to apply them. Presents reliable, accurate, and clear stepwise protocols Includes all new coverage of genomics, proteomics (identification of targets and characterization of functional domains), PCR applications such as mutagenesis, and microarray technology and applications Discusses the biochemistry underlying commonly seen kits, in order that the reader will be able to use the kits to solve non–classical problems Includes new chapters on basic methods in microbiology, basic methods in cellular biology, methods for studying protein–protein interactions, SAGE, phage display, and methods for modifying the host genome Includes numerous tables and an appendix of companies and useful websites
Methods In Enzymology
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Author : Sidney P. Colowick
language : en
Publisher:
Release Date : 1987
Methods In Enzymology written by Sidney P. Colowick and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Molecular cloning categories.
A Complete Guide To Gene Cloning From Basic To Advanced
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Author : Nayana Patil
language : en
Publisher: Springer Nature
Release Date : 2022-04-26
A Complete Guide To Gene Cloning From Basic To Advanced written by Nayana Patil and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-26 with Science categories.
This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.
Cloning
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Author : Stephen D. Fairbanks
language : en
Publisher: Nova Publishers
Release Date : 2004
Cloning written by Stephen D. Fairbanks and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.
Molecular Cloning
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Author : Gregory G. Brown
language : en
Publisher: BoD – Books on Demand
Release Date : 2011-10-12
Molecular Cloning written by Gregory G. Brown and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-12 with Medical categories.
The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.
Dna Cloning And Assembly
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Author : Sunil Chandran
language : en
Publisher: Humana
Release Date : 2021-09-02
Dna Cloning And Assembly written by Sunil Chandran and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-02 with Medical categories.
This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Understanding Dna And Gene Cloning
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Author : Karl Drlica
language : en
Publisher: John Wiley & Sons
Release Date : 2004
Understanding Dna And Gene Cloning written by Karl Drlica and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
Publisher's description: With DNA and gene cloning all over the news, readers need to understand the ongoing genetic revolution. In this highly acclaimed guide, Karl Drlica fully explains the basic science and technology readers need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies.
Understanding Dna And Gene Cloning
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Author : Karl Drlica
language : en
Publisher: Turtleback
Release Date : 2003-04-01
Understanding Dna And Gene Cloning written by Karl Drlica and has been published by Turtleback this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-04-01 with Science categories.
With DNA and gene cloning all over the news, readers need to understand the ongoing genetic revolution. In this highly acclaimed guide, Karl Drlica fully explains the basic science and technology readers need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies. The new edition is now completely up-to-date.