Molecular Cloning
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Molecular Cloning
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Author : Gregory G. Brown
language : en
Publisher: BoD – Books on Demand
Release Date : 2011-10-12
Molecular Cloning written by Gregory G. Brown and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-12 with Medical categories.
The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.
Gene Cloning
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Author : Julia Lodge
language : en
Publisher: Garland Science
Release Date : 2007-01-24
Gene Cloning written by Julia Lodge and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-24 with Science categories.
The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.
Introduction To Molecular Cloning Techniques
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Author : G. Lucotte
language : en
Publisher: Wiley-VCH
Release Date : 1993-07-20
Introduction To Molecular Cloning Techniques written by G. Lucotte and has been published by Wiley-VCH this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-07-20 with Science categories.
Introduction to Molecular Cloning Techniques is a concise summary of the basis principles and methods used in genetic engineering and is intended for students and research technicians involved in microbiology, molecular biology, genetics, bioengineering, biotechnology, and chemical engineering. Focusing entirely on the most widely-used host, E. coli, the book provides extensive descriptions of cloning vectors and essential recombinant DNA methodologies, as well as discusses the steps involved in the construction of genomic, cDNA, and cosmid librairies. The chapters describe important aspects of molecular cloning by providing the necessary fundamental biochemistry and microbiology background to clearly introduce the pertinent genetic engineering concepts. Examples of routinely used experimental protocols and solved problems are included at the end of each chapter in order to extend their theoretical content and familiarize the reader with laboratory technicians and the conditions for their use.
A Practical Guide To Molecular Cloning
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Author : Bernard V. Perbal
language : en
Publisher: Wiley-Interscience
Release Date : 1984
A Practical Guide To Molecular Cloning written by Bernard V. Perbal and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Genetic engineering categories.
Presents techniques tested at the Curie Institute and other leading labs and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. Offers detailed explanation of protocols, allowing the isolation, cloning, and expression of genes from living species. Presents up-to-date techniques on sequencing, in vitro expression of cloned gene, and use of computers for study of nucleic acids, and is the only book that shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes. Contains 235 figures and 80 tables.
Gene Cloning And Manipulation
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Author : Christopher Howe
language : en
Publisher: Cambridge University Press
Release Date : 2007-07-12
Gene Cloning And Manipulation written by Christopher Howe and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-12 with Medical categories.
Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Vectors
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Author : Raymond L. Rodriguez
language : en
Publisher: Butterworth-Heinemann
Release Date : 2014-05-20
Vectors written by Raymond L. Rodriguez and has been published by Butterworth-Heinemann this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-20 with Science categories.
Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.
Gene Cloning And Genomics Principles And Applications
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Author : Dr. RAVISHANKAR B.V.
language : en
Publisher: Lulu.com
Release Date :
Gene Cloning And Genomics Principles And Applications written by Dr. RAVISHANKAR B.V. and has been published by Lulu.com this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Molecular Cloning Of Hormone Genes
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Author : Joel F. Habener
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Molecular Cloning Of Hormone Genes written by Joel F. Habener and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry onic development. During the past ten years, refinements in the techniques of recombinant DNA technology have resulted in the cloning of genes encoding approximately 50 different hormonal and regulatory peptides, including those in which the peptides themselves and the mRNAs encoding the peptides are present in only trace amounts in the tissues of origin. In addition to provid ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors. The encoding of multiple peptides in a single mono cistronic mRNA appears to be a genetic mechanism for the gener ation of biologic diversification without requiring amplification of gene sequences. Two of the objectives in the assembly of this book are to pre sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related regulatory peptides, and to provide an account of the various ap proaches that have been used to identify and select the cloned genes encoding these polypeptides. The contents of the two in troductory chapters are intended to provide the reader with a brief background of the approaches to gene cloning and the struc ture and expression of hormone-encoding genes.
Molecular Cloning
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Author : Joseph Sambrook
language : en
Publisher:
Release Date : 2001
Molecular Cloning written by Joseph Sambrook and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Medical categories.
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
Understanding Dna And Gene Cloning
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Author : Karl Drlica
language : en
Publisher: John Wiley & Sons
Release Date : 2004
Understanding Dna And Gene Cloning written by Karl Drlica and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
Publisher's description: With DNA and gene cloning all over the news, readers need to understand the ongoing genetic revolution. In this highly acclaimed guide, Karl Drlica fully explains the basic science and technology readers need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies.