Gene Cloning And Manipulation
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Gene Cloning And Manipulation
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Author : Christopher Howe
language : en
Publisher: Cambridge University Press
Release Date : 2007-07-12
Gene Cloning And Manipulation written by Christopher Howe and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-12 with Medical categories.
Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Textbook On Cloning Expression And Purification Of Recombinant Proteins
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Author : Kakoli Bose
language : en
Publisher: Springer Nature
Release Date : 2022-01-25
Textbook On Cloning Expression And Purification Of Recombinant Proteins written by Kakoli Bose and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-25 with Medical categories.
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
The Abcs Of Gene Cloning
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Author : Dominic Wong
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-31
The Abcs Of Gene Cloning written by Dominic Wong and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-31 with Science categories.
Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.
Principles Of Gene Manipulation
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Author : R. W. Old
language : en
Publisher: Univ of California Press
Release Date : 1980
Principles Of Gene Manipulation written by R. W. Old and has been published by Univ of California Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Science categories.
Gene Cloning And Manipulation
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Author : Christopher Howe
language : en
Publisher:
Release Date : 1995
Gene Cloning And Manipulation written by Christopher Howe and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
An Introduction To Genetic Engineering
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Author : Desmond S. T. Nicholl
language : en
Publisher: Cambridge University Press
Release Date : 2002-02-07
An Introduction To Genetic Engineering written by Desmond S. T. Nicholl and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-02-07 with Medical categories.
The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved.
Manipulation And Expression Of Recombinant Dna
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Author : Sue Carson
language : en
Publisher: Elsevier
Release Date : 2005-12-15
Manipulation And Expression Of Recombinant Dna written by Sue Carson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-15 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Gene Cloning And Manipulation
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Author : Professor of Economics School of Oriental and African Studies Christopher Howe
language : en
Publisher:
Release Date : 2014-05-14
Gene Cloning And Manipulation written by Professor of Economics School of Oriental and African Studies Christopher Howe and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-14 with Molecular cloning categories.
Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Genetic Manipulation
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Author : J. M. Grange
language : en
Publisher: John Wiley & Sons
Release Date : 1991-01-08
Genetic Manipulation written by J. M. Grange and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-01-08 with Science categories.
Genetic manipulation is no longer the province of the specialized researcher. It is finding widespread application in all fields of medicine and biology. Nevertheless, application of these relatively new techniques to new areas of research is often fraught with unexpected problems and difficulties. Based on the Society for Applied Bacteriology's Autumn 1989 Conference, this unique volume covers a wide and very up-to-date range of techniques used in genetic engineering. These include the isolation and analysis of DNA and RNA from cells and tissues, the selection and use of phage and plasmic vectors for cloning DNA, the cloning procedures, the production and screening of genomic libraries, the production and use of DNA probes, the polymerase chain reaction and the synthesis of 'designer' genes. This volume contains many examples of the applications of the above and other techniques for genetic manipulation, to subjects as diverse as plant pathology, forensic science, bacterial taxonomy, cardiac research, diagnostic microbiology, food hygiene and sewage treatment.