Textbook On Cloning Expression And Purification Of Recombinant Proteins
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Textbook On Cloning Expression And Purification Of Recombinant Proteins
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Author : Kakoli Bose
language : en
Publisher: Springer Nature
Release Date : 2022-01-25
Textbook On Cloning Expression And Purification Of Recombinant Proteins written by Kakoli Bose and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-25 with Medical categories.
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Fundamentals Of Recombinant Protein Production Purification And Characterization
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Author : Deepti Yadav
language : en
Publisher: Elsevier
Release Date : 2024-09-10
Fundamentals Of Recombinant Protein Production Purification And Characterization written by Deepti Yadav and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-09-10 with Science categories.
Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins. - Includes step-by-step reproducible protocols while also providing updated information on the rationale and latest developments in expression systems - Can also be used as a handbook for protein expression and purification as expression systems and chromatographic methods are explained in detail - Consists of notes on troubleshooting from the eminent researchers in the field - Provides comprehensive information on protein production, purification and characterization in a single volume - Describes different purification methods for comparatively difficult to obtain proteins - Brings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins
Basic Biotechniques For Bioprocess And Bioentrepreneurship
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Author : Arvind Kumar Bhatt
language : en
Publisher: Academic Press
Release Date : 2023-02-03
Basic Biotechniques For Bioprocess And Bioentrepreneurship written by Arvind Kumar Bhatt and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-03 with Science categories.
Basic Biotechniques for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from the basic laboratory techniques to scale-up, process development, demonstration, and finally its commercialization. The book compiles currently scattered materials on this topic and updates this information based on practical experience and requirements. The book will be an ideal source for new entrepreneurs who wish to start their own commercial units. - Offers guidance for readers/researchers/start-ups/entrepreneurs on how to develop new microbiological and biotechnical processes - Focuses on basic knowledge and possible solutions to the practical difficulties at all levels in one place through understanding of basic techniques in lab, during bioprocess development, commercialization, technology transfer, marketing, and others which is presently not available in the field - Provides multifaceted coverage, with industry insights from experienced practitioners and leaders in the field - Gives possible best solutions to the practical difficulties at all levels, i.e. lab, scaleup, and commercial stage - Addresses ethical and other regulatory issues
Antimicrobial Peptides
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Author : Luis H. Reyes
language : en
Publisher: Elsevier
Release Date : 2024-11-22
Antimicrobial Peptides written by Luis H. Reyes and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-11-22 with Science categories.
Antimicrobial Peptides: A Roadmap for Accelerating Discovery and Development covers the most important efforts of scientists and engineers worldwide to accelerate the process of discovery, production, and eventual market penetration of more potent antimicrobial peptides. These efforts have been fueled by emerging technologies such as artificial intelligence and data science, molecular and CFD simulations, easy-to-use process simulation packages, microfluidics, 3D-printing, among many others. Such technologies can now be implemented and scaled up quickly and at relatively low cost in low-budget production facilities, critical to moving to sustainable and marketable products worldwide.Discovering novel antimicrobial peptides rationally and cost-effectively has emerged as one of the significant challenges of modern biotechnology. Thus far, this process has been tedious and costly, resulting in molecules with activities far below those needed to address the current challenge of microbial resistance to antibiotics that takes the lives of thousands of people around the world every year. Finally, the book also highlights how multidisciplinary teams have assembled to address the challenges of manufacturing, biological testing, and clinical trials to finally reach complete translation. - Covers computational tools (including emerging artificial intelligence algorithms) and microfluidic systems for discovery and high-throughput screening of AMPs - Discusses the application of bioprocess engineering scale-up approaches for AMPs' production and purification with the aid of process simulation tools and rapid prototyping - Highlights user-centered design and formulation of products with AMPs - Describes the whole pipeline for AMPs production
Plant Proteomics
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Author : Aryadeep Roychoudhury
language : en
Publisher: CRC Press
Release Date : 2024-06-03
Plant Proteomics written by Aryadeep Roychoudhury and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-03 with Science categories.
There have been several advancements made in high-throughput protein technologies creating immense possibilities for studying proteomics on a large scale. Researchers are exploring various proteomic techniques to unravel the mystery of plant stress tolerance mechanisms. Plant Proteomics: Implications in Growth, Quality Improvement, and Stress Resilience introduces readers to techniques and methodologies of proteomics and explains different physiological phenomena in plants and their responses to various environmental cues and defense mechanisms against pathogens. The main emphasis is on research involving applications of proteomics to understand different aspects of the life cycle of plant species including dormancy, flowering, photosynthetic efficiency, nitrogen assimilation, accumulation of nutritional parameters, secondary metabolite production, reproduction and grain yield as well as signalling responses during abiotic and biotic stresses. The book takes a unique approach, encompassing high throughput and sophisticated proteomic techniques while integrating proteomics with other “omics.” Features: Integrates the branch of proteomics with other “omics” approaches including genomics and metabolomics, giving a holistic view of the overall “omics” approaches. Covers various proteomics approaches for the identification of biological processes, future perspectives, and upcoming applications to identify diverse genes in plants. Presents readers with various proteomics tools for the improvement of plant growth, quality, and resilience against climate change, and pathogen infection. Enables researchers in identifying novel proteins that could be used as target to generate plants with improved traits. Prof. Aryadeep Roychoudhury is currently working as Professor in the Discipline of Life Sciences, Indira Gandhi National Open University, New Delhi, India. Earlier, he served as Assistant Professor at the Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, West Bengal, India. He received his B.Sc. (Hons.) in Botany from Presidency College, Kolkata, and M.Sc. in Biophysics and Molecular Biology, University of Calcutta, West Bengal, India. He earned his Ph.D. from Jadavpur University, Kolkata in the area of stress biology in plants. Following his Ph.D. work, he joined as Research Associate (Post doctorate) at the University of Calcutta, pursuing translational research on transgenic rice. He is presently involved in active research in the field of abiotic stress responses in plants with perspectives to the physiology, molecular biology and cell signaling under diverse stress conditions. He has 23 years of research experience in the concerned discipline. Prof. Roychoudhury has handled several government-funded projects as principal investigator and supervised five Ph.D. students as principal investigator. He has published over 250 articles in peer-reviewed journals and chapters in books of international and national repute. He has edited many books with Wiley, Elsevier, and Springer, and has also handled Special Issues as Guest Editor for several renowned international journals. He is a regular reviewer of articles in high-impact, international journals, Life Member of different scientific associations and societies, and the recipient of the Young Scientist Award 2019, conferred upon him by International Foundation for Environment and Ecology, at University of Allahabad, Prayagraj, Uttar Pradesh. His name is included in the Stanford University’s List of World’s Top 2% Influential Scientists.
Crispr Cas Based Genome Editing For Treating Human Diseases Part B
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Author :
language : en
Publisher: Elsevier
Release Date : 2025-01-01
Crispr Cas Based Genome Editing For Treating Human Diseases Part B written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-01-01 with Science categories.
CRISPR-Cas-Based Genome Editing for Treating Human Diseases, Part B represents CRISPR-Cas systems for genome editing. Currently, CRISPR-Cas systems have been proven to be a key technology for targeted genome editing, which is acting as a simple, rapid, and cost-effective solution. Chapters in this release include Current approaches in CRISPR –Cas systems for metabolic disorders, Recent progress in CRISPR –Cas systems for CRISPR for retinal diseases, Recent progress in CRISPR –Cas systems for cataract and blindness, Advances in CRISPR –Cas systems for muscular dystrophy, Recent development in CRISPR –Cas systems for cardiac disease, Current approaches in CRISPR –Cas systems for diabetes, and much more.Additional sections cover Advances in CRISPR –Cas systems for liver disease, Advances in CRISPR –Cas systems for lung disease, Advances in CRISPR –Cas systems for kidney diseases, Current progress in CRISPR –Cas systems for rare disease, Advances in CRISPR –Cas systems for heredity disease, Recent progress in CRISPR –Cas systems for neurological disorders, and CRISPR challenges in clinical developments. - Provides CRISPR-Cas systems for metabolic disorders, retinal disease cataracts, and blindness - Offer updates on muscular dystrophy, cardiac disease, diabetes, and liver disease - Includes content on lung disease, kidney diseases, rare diseases, heredity diseases, neurological disorders, and CRISPR challenges in clinical developments
Analytical Methods In Chemical Analysis
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Author : Shikha Kaushik
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2023-06-19
Analytical Methods In Chemical Analysis written by Shikha Kaushik and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-06-19 with Technology & Engineering categories.
Analytical Chemistry is important and applied, experimental field of science that employs different instruments, and methods for the collection, separation, identification, and quantification of various organic, inorganic, and biological molecules. This interdisciplinary branch is based not only on chemistry but also on other disciplines such as biology, physics, pharmaceutical, and many areas of technology. The book is organized into six sections and provides information pertinent to the important techniques, and methods employed in analytical chemistry. It covers the basic concepts of qualitative and quantitative analysis, spectrochemical methods of analysis, along with thermal- and electroanalytical methods. Qualitative analysis identifies analytes, while quantitative analysis determines the concentration or numerical amount of the molecules under study. This book also exposes students to the different laws of spectroscopy, and various electronic transitions that occur in the different regions of the electromagnetic spectra. The main objective of this work is to develop an understanding and make learners familiar with the basic analytical methods employed in the chemical analysis of various compounds.
Essential Laboratory Techniques And Biochemical Analysis
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Author : Darshan Malik
language : en
Publisher: CRC Press
Release Date : 2025-09-09
Essential Laboratory Techniques And Biochemical Analysis written by Darshan Malik and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-09-09 with Science categories.
This laboratory manual comprehensively reviews essential laboratory practices and different biochemistry protocols. The initial chapters of the book provide an overview of lab safety protocols, focusing on the importance of accuracy and precision in experimental procedures. It covers essential topics, such as laboratory setup, proper handling and maintenance of lab apparatus, and waste disposal. It provides a detailed exploration of spectrophotometry principles and assays, along with comprehensive cell biology techniques, including staining and microscopy. The book also addresses qualitative and quantitative analyses of carbohydrates, amino acids, proteins, and lipids, providing methods for extraction and characterization. It further details the extraction, purification, and characterization of enzymes and presents enzymatic assays and studies on enzyme kinetics, providing a comprehensive understanding of enzyme activity and regulation. The final section introduces hematology techniques, including blood smear preparation and various blood parameter determinations. It also covers forensic tests for blood detection and serum protein electrophoresis. This book is useful for graduate and postgraduate students of biochemistry, molecular biology, and microbiology.
Innovations And Implications In Molecular Diagnostics
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Author : Qamre Alam
language : en
Publisher: CRC Press
Release Date : 2025-09-10
Innovations And Implications In Molecular Diagnostics written by Qamre Alam and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-09-10 with Medical categories.
This book introduces the field of molecular diagnostics, tracing its evolution from the earliest days of genetic research to cutting-edge advancements. It begins with a historical overview, setting the stage for an understanding of the foundational principles and techniques that underpin current practices in DNA analysis. It also provides the intricate details of various molecular techniques, such as advanced polymerase chain reaction (PCR) methods and next-generation sequencing, and their impact in the field of genetics and diagnostics. The book also explores the significant role of bioinformatics as the backbone of modern diagnostics and examines the changes brought about by high-throughput technologies and nanotechnology in molecular research and diagnostics. The importance of molecular imaging techniques in visualizing biological processes is discussed, along with the advancements in RNA analysis and their diagnostic implications. The book addresses the emerging fields of metabolomics and proteomics in molecular diagnostics and the transformative impact of artificial intelligence in this field. The book concludes with a thoughtful discussion on the ethical and regulatory considerations in molecular diagnostics. This book is intended for researchers and students of the fields of molecular diagnostics, biotechnology, and genomics. Key Features: Provides historical overview and recent advancements in the field of molecular diagnostics Explores foundational principles and advanced techniques in DNA analysis, including detailed insights into PCR methods Covers the applications and impact of next-generation sequencing in genetics and diagnostics Discusses the revolutionary influence of high-throughput technologies and nanotechnology in molecular research and diagnostics Examines the roles of metabolomics, proteomics, and artificial intelligence in molecular diagnostics
Bioreactor Technology In Food Processing
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Author : Rosane F. Schwan
language : en
Publisher: CRC Press
Release Date : 2024-11-29
Bioreactor Technology In Food Processing written by Rosane F. Schwan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-11-29 with Technology & Engineering categories.
Bioreactor Technology in Food Processing brings peculiarities, specificities, and updates on bioreactors and bioprocesses related to food and beverage production. The 26 chapters of this book are the result of the participation of more than 70 professionals, including professors, researchers, and experts from the industrial sector from different countries around the world. The chapters cover such topics as history, classification, scale-up, analytical tools, and mathematical and kinetic models for the operation of bioreactors in the food industry. In addition, chapters detail the characteristics of bioreactors for the production of food (bread, cheese, and coffee fermentation) and fermented beverages (beer, wine), distilled beverages, and organic compounds such as enzymes, acids, aromas, and pigments (biocolorants), among others. Key Features: Describes the basic and applied aspects of bioreactor in food processing Gathers information on bioreactors that is scattered in different journals and monographs as reviews and research articles Covers various types of bioreactors including stirred tank, airlift, photo-bioreactor, and disposable bioreactors Gives a broad overview of what exactly is involved in designing a bioreactor and optimizing its performance and finally their applications in the food processing industry The broad interdisciplinary approach of this book will certainly make your reading very interesting, and we hope that it can contribute to knowledge and instigate creative thinking to overcome the challenges that food bioprocessing brings us.