Handbook Of Electroporation
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Handbook Of Electroporation
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Author : Damijan Miklavčič
language : en
Publisher: Springer
Release Date : 2017-09-14
Handbook Of Electroporation written by Damijan Miklavčič and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-09-14 with Technology & Engineering categories.
This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of 156 chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The Handbook's 276 authors have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment. An Editorial Board of distinguished scholars from across the world has selected and reviewed the various chapters to ensure the highest quality of this Handbook. The book was edited by an international team of Section Editors: P. Thomas Vernier, Boris Rubinsky, Juergen Kolb, Damijan Miklavcic, Marie-Pierre Rols, Javier Raso, Richard Heller, Gregor Serša, Dietrich Knorr, and Eugene Vorobiev.
Guide To Electroporation And Electrofusion
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Author : Donald C. Chang
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Guide To Electroporation And Electrofusion written by Donald C. Chang and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells. Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. Key Features * Covers fundamentals of electroporation and electrofusion in detail * Molecular events * Mechanisms * Kinetics * Gives extensive practical information * The latest applications * Controlling parameters to maximize efficiency * Available instrumentation * Presents applications of electroporation and electrofusion in current research situations * State-of-the-art modifications to electrical pulses and generators * Application of electroporation and electrofusion to unique, alternative cell and tissue types * Gives straightforward, detailed, easy-to-follow protocols for * Formation of human hybridomas * Introduction of genetic material into plant cells and pollen * Transfection of mammalian cells * Transformation of bacteria, plants, and yeast * Production of altered embryos * Optimization of electroporation by using reporter genes * Comprehensive and up-to-date * Convenient bench-top format * Approximately 125 illustrations complement the text * Complete references with article titles * Written by leading authorities in electroporation and electrofusion
Electrotransformation Of Bacteria
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Author : Natalie Eynard
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Electrotransformation Of Bacteria written by Natalie Eynard and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
In this manual, protocols for the transformation of about 40 strains of bacteria are described, with the emphasis placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows users to modify and adapt each protocol to their own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.
Irreversible Electroporation
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Author : Boris Rubinsky
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-11-25
Irreversible Electroporation written by Boris Rubinsky and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-25 with Technology & Engineering categories.
Non-thermal irreversible electroporation is a new minimally invasive surgical p- cedure with unique molecular selectivity attributes – in fact it may be considered the first clinical molecular surgery procedure. Non-thermal irreversible electro- ration is a molecular selective mode of cell ablation that employs brief electrical fields to produce nanoscale defects in the cell membrane, which can lead to cell death, without an effect on any of the other tissue molecules. The electrical fields can be produced through contact by insertion of electrode needles around the undesirable tissue and non-invasively by electromagnetic induction. This new - dition to the medical armamentarium requires the active involvement and is of interest to clinical physicians, medical researchers, mechanical engineers, che- cal engineers, electrical engineers, instrumentation designers, medical companies and many other fields and disciplines that were never exposed in their training to irreversible electroporation or to a similar concept. This edited book is designed to be a comprehensive introduction to the field of irreversible electroporation to those that were not exposed or trained in the field before and can also serve as a reference manual. Irreversible electroporation is broad and interdisciplinary. Therefore, we have made an attempt to cover every one of the various aspects of the field from an introductory basic level to state of the art.
Plant Cell Electroporation And Electrofusion Protocols
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Author : Jac A. Nickoloff
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Plant Cell Electroporation And Electrofusion Protocols written by Jac A. Nickoloff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna tives to electric field-based methods, citing the advantages and limita tions of the various methods for achieving specific goals.
Electroporation And Electrofusion In Cell Biology
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Author : C.A. Jordan
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Electroporation And Electrofusion In Cell Biology written by C.A. Jordan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
1st World Congress On Electroporation And Pulsed Electric Fields In Biology Medicine And Food Environmental Technologies
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Author : Tomaz Jarm
language : en
Publisher: Springer
Release Date : 2015-08-31
1st World Congress On Electroporation And Pulsed Electric Fields In Biology Medicine And Food Environmental Technologies written by Tomaz Jarm and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-08-31 with Technology & Engineering categories.
This volume presents the proceedings of the 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies (WC2015). The congress took place in Portorož, Slovenia, during the week of September 6th to 10th, 2015. The scientific part of the Congress covered different aspects of electroporation and related technologies and included the following main topics: · Application of pulsed electric fields technology in food: challenges and opportunities · Electrical impedance measurement for assessment of electroporation yield · Electrochemistry and electroporation · Electroporation meets electrostimulation · Electrotechnologies for food and biomass treatment · Food and biotechnology applications · In vitro electroporation - basic mechanisms · Interfacial behaviour of lipid-assemblies, membranes and cells in electric fields · Irreversible electroporation in clinical use · Medical applications: electrochemotherapy · Medical applications: gene therapy · Non-electric field-based physical methods inducing cell poration and enhanced molecule transfer · Non-thermal plasmas for food safety, environmental applications and medical treatments · PEF for the food industry: fundamentals and applications · PEF proce ss integration - complex process chains and process combinations in the food industry · Predictable animal models · Pulsed electric fields and electroporation technologies in bioeconomy · Veterinary medical applications
Advanced Electroporation Techniques In Biology And Medicine
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Author : Andrei G. Pakhomov
language : en
Publisher: CRC Press
Release Date : 2010-06-02
Advanced Electroporation Techniques In Biology And Medicine written by Andrei G. Pakhomov and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-06-02 with Medical categories.
A reflection of the intense study of the effects of electromagnetic fields on living tissues that has taken place during the last decades, Advanced Electroporation Techniques in Biology and Medicine summarizes most recent experimental findings and theories related to permeabilization of biomembranes by pulsed electric fields. Edited by experts and
Handbook Of Biological Effects Of Electromagnetic Fields Third Edition 2 Volume Set
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Author : Charles Polk
language : en
Publisher: CRC Press
Release Date : 1995-12-21
Handbook Of Biological Effects Of Electromagnetic Fields Third Edition 2 Volume Set written by Charles Polk and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-12-21 with Technology & Engineering categories.
The first edition of this book has been recognized as the standard reference on biological effects of electric and magnetic fields from DC to microwaves. But much has changed in this science since the book's original publication in 1986. With contributions from eighteen leading researchers, this latest edition includes authoritative discussions of many new developments and will quickly become the new, must-have resource handbook. Dielectric properties of biological tissue are thoroughly examined, followed by chapters on physical mechanisms and biological effects of static and extremely low frequency magnetic fields. New chapters on topics that were treated very briefly in the first edition now receive extensive treatment. These topics include electric and magnetic fields for bone and soft tissue repair, electroporation, and epidemiology of ELF health effects. The chapter on computer methods for predicting field intensity has been substantially revised to describe new numerical techniques developed within the last few years and includes calculations of power absorbed in the human head from cellular telephones. The chapter discussing experimental results on RF interaction with living matter now contains information on effects of very high power, very short duration pulses. A new appendix on safety standards is based on the latest publications of governmental, as well as quasi-governmental organizations (such as the U.S. Council on Radiation Protection) in the United States, Europe, and Australia. With all its revisions, this updated version of the CRC Handbook of Biological Effects of Electromagnetic Fields provides the most comprehensive overview available of this rapidly changing science.
Cell Biology
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Author : Julio E Celis
language : en
Publisher: Academic Press
Release Date : 2014-05-10
Cell Biology written by Julio E Celis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-10 with Science categories.
Cell Biology: A Laboratory Handbook, Volume 3 is a handbook on cell biology and covers topics ranging from transfer of macromolecules and small molecules to cloning of embryos, transgenics, and gene targeting. Cell-free extracts, permeabilized cell systems, and expression systems are also discussed, along with proteins. Comprised of 58 chapters, this volume begins with a detailed account of microinjection of RNA, DNA, and proteins into somatic cells, followed by an analysis of computer-automated capillary microinjection of macromolecules into living cells. The reader is then introduced to syringe loading as a method for inserting macromolecules into cells in suspension; electroporation of cells; and the use of liposomes in drug targeting. Subsequent chapters focus on the cloning of rabbit embryos by nuclear transplantation; gene targeting by homologous recombination in embryonic stem cells; production and isolation of recombinant viruses; and gel electrophoresis. This book will be of interest to geneticists and molecular biologists.